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Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma

Methyl gallate is a phenolic compound mainly found in medicinal plants. It has been reported to its anticancer activity in various tumors. In this study, we aimed to demonstrate the antitumor effect of methyl gallate in the melanoma mouse model and B16F10 cells. Our results showed that methyl gallat...

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Autores principales: Park, Jeong-Ki, Yoo, Min-Jae, Jang, Hyuk, Park, Sang-Youel, Choi, Jawun, Seol, Jae-Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9470304/
https://www.ncbi.nlm.nih.gov/pubmed/36110191
http://dx.doi.org/10.1155/2022/6295910
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author Park, Jeong-Ki
Yoo, Min-Jae
Jang, Hyuk
Park, Sang-Youel
Choi, Jawun
Seol, Jae-Won
author_facet Park, Jeong-Ki
Yoo, Min-Jae
Jang, Hyuk
Park, Sang-Youel
Choi, Jawun
Seol, Jae-Won
author_sort Park, Jeong-Ki
collection PubMed
description Methyl gallate is a phenolic compound mainly found in medicinal plants. It has been reported to its anticancer activity in various tumors. In this study, we aimed to demonstrate the antitumor effect of methyl gallate in the melanoma mouse model and B16F10 cells. Our results showed that methyl gallate decreased cell viability and induced apoptosis by increasing the expression of cleaved caspase3 in B16F10 cells and prevented cell migration and tube formation in human umbilical vein endothelial cells. In B16F10 cell-inoculated mice, methyl gallate not only decreased tumor volume by 30% but also significantly reduced tumor vessel density and pericyte coverage. Moreover, methyl gallate diminished by close to 50% the expression of cytokeratin and LYVE-1 in mouse right inguinal lymph nodes, indicating that methyl gallate could suppress metastasis. In conclusion, this study suggests that methyl gallate inhibits tumor development by inducing apoptosis and blocking tumor angiogenesis and metastasis and might be considered a therapeutic agent for melanoma.
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spelling pubmed-94703042022-09-14 Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma Park, Jeong-Ki Yoo, Min-Jae Jang, Hyuk Park, Sang-Youel Choi, Jawun Seol, Jae-Won Evid Based Complement Alternat Med Research Article Methyl gallate is a phenolic compound mainly found in medicinal plants. It has been reported to its anticancer activity in various tumors. In this study, we aimed to demonstrate the antitumor effect of methyl gallate in the melanoma mouse model and B16F10 cells. Our results showed that methyl gallate decreased cell viability and induced apoptosis by increasing the expression of cleaved caspase3 in B16F10 cells and prevented cell migration and tube formation in human umbilical vein endothelial cells. In B16F10 cell-inoculated mice, methyl gallate not only decreased tumor volume by 30% but also significantly reduced tumor vessel density and pericyte coverage. Moreover, methyl gallate diminished by close to 50% the expression of cytokeratin and LYVE-1 in mouse right inguinal lymph nodes, indicating that methyl gallate could suppress metastasis. In conclusion, this study suggests that methyl gallate inhibits tumor development by inducing apoptosis and blocking tumor angiogenesis and metastasis and might be considered a therapeutic agent for melanoma. Hindawi 2022-09-06 /pmc/articles/PMC9470304/ /pubmed/36110191 http://dx.doi.org/10.1155/2022/6295910 Text en Copyright © 2022 Jeong-Ki Park et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Park, Jeong-Ki
Yoo, Min-Jae
Jang, Hyuk
Park, Sang-Youel
Choi, Jawun
Seol, Jae-Won
Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma
title Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma
title_full Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma
title_fullStr Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma
title_full_unstemmed Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma
title_short Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma
title_sort methyl gallate suppresses tumor development by increasing activation of caspase3 and disrupting tumor angiogenesis in melanoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9470304/
https://www.ncbi.nlm.nih.gov/pubmed/36110191
http://dx.doi.org/10.1155/2022/6295910
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