Cargando…
Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma
Methyl gallate is a phenolic compound mainly found in medicinal plants. It has been reported to its anticancer activity in various tumors. In this study, we aimed to demonstrate the antitumor effect of methyl gallate in the melanoma mouse model and B16F10 cells. Our results showed that methyl gallat...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9470304/ https://www.ncbi.nlm.nih.gov/pubmed/36110191 http://dx.doi.org/10.1155/2022/6295910 |
_version_ | 1784788810911973376 |
---|---|
author | Park, Jeong-Ki Yoo, Min-Jae Jang, Hyuk Park, Sang-Youel Choi, Jawun Seol, Jae-Won |
author_facet | Park, Jeong-Ki Yoo, Min-Jae Jang, Hyuk Park, Sang-Youel Choi, Jawun Seol, Jae-Won |
author_sort | Park, Jeong-Ki |
collection | PubMed |
description | Methyl gallate is a phenolic compound mainly found in medicinal plants. It has been reported to its anticancer activity in various tumors. In this study, we aimed to demonstrate the antitumor effect of methyl gallate in the melanoma mouse model and B16F10 cells. Our results showed that methyl gallate decreased cell viability and induced apoptosis by increasing the expression of cleaved caspase3 in B16F10 cells and prevented cell migration and tube formation in human umbilical vein endothelial cells. In B16F10 cell-inoculated mice, methyl gallate not only decreased tumor volume by 30% but also significantly reduced tumor vessel density and pericyte coverage. Moreover, methyl gallate diminished by close to 50% the expression of cytokeratin and LYVE-1 in mouse right inguinal lymph nodes, indicating that methyl gallate could suppress metastasis. In conclusion, this study suggests that methyl gallate inhibits tumor development by inducing apoptosis and blocking tumor angiogenesis and metastasis and might be considered a therapeutic agent for melanoma. |
format | Online Article Text |
id | pubmed-9470304 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-94703042022-09-14 Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma Park, Jeong-Ki Yoo, Min-Jae Jang, Hyuk Park, Sang-Youel Choi, Jawun Seol, Jae-Won Evid Based Complement Alternat Med Research Article Methyl gallate is a phenolic compound mainly found in medicinal plants. It has been reported to its anticancer activity in various tumors. In this study, we aimed to demonstrate the antitumor effect of methyl gallate in the melanoma mouse model and B16F10 cells. Our results showed that methyl gallate decreased cell viability and induced apoptosis by increasing the expression of cleaved caspase3 in B16F10 cells and prevented cell migration and tube formation in human umbilical vein endothelial cells. In B16F10 cell-inoculated mice, methyl gallate not only decreased tumor volume by 30% but also significantly reduced tumor vessel density and pericyte coverage. Moreover, methyl gallate diminished by close to 50% the expression of cytokeratin and LYVE-1 in mouse right inguinal lymph nodes, indicating that methyl gallate could suppress metastasis. In conclusion, this study suggests that methyl gallate inhibits tumor development by inducing apoptosis and blocking tumor angiogenesis and metastasis and might be considered a therapeutic agent for melanoma. Hindawi 2022-09-06 /pmc/articles/PMC9470304/ /pubmed/36110191 http://dx.doi.org/10.1155/2022/6295910 Text en Copyright © 2022 Jeong-Ki Park et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Park, Jeong-Ki Yoo, Min-Jae Jang, Hyuk Park, Sang-Youel Choi, Jawun Seol, Jae-Won Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma |
title | Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma |
title_full | Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma |
title_fullStr | Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma |
title_full_unstemmed | Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma |
title_short | Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma |
title_sort | methyl gallate suppresses tumor development by increasing activation of caspase3 and disrupting tumor angiogenesis in melanoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9470304/ https://www.ncbi.nlm.nih.gov/pubmed/36110191 http://dx.doi.org/10.1155/2022/6295910 |
work_keys_str_mv | AT parkjeongki methylgallatesuppressestumordevelopmentbyincreasingactivationofcaspase3anddisruptingtumorangiogenesisinmelanoma AT yoominjae methylgallatesuppressestumordevelopmentbyincreasingactivationofcaspase3anddisruptingtumorangiogenesisinmelanoma AT janghyuk methylgallatesuppressestumordevelopmentbyincreasingactivationofcaspase3anddisruptingtumorangiogenesisinmelanoma AT parksangyouel methylgallatesuppressestumordevelopmentbyincreasingactivationofcaspase3anddisruptingtumorangiogenesisinmelanoma AT choijawun methylgallatesuppressestumordevelopmentbyincreasingactivationofcaspase3anddisruptingtumorangiogenesisinmelanoma AT seoljaewon methylgallatesuppressestumordevelopmentbyincreasingactivationofcaspase3anddisruptingtumorangiogenesisinmelanoma |