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Metagenomic next-generation sequencing provides prognostic warning by identifying mixed infections in nocardiosis
Nocardia is an opportunistic pathogen that mainly involves immunosuppressed patients and causes a high mortality rate. As an emerging approach to detect infectious pathogens, metagenomic next-generation sequencing (mNGS) was reported in the detection of Nocardia. However, there is no evidence demons...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471186/ https://www.ncbi.nlm.nih.gov/pubmed/36118026 http://dx.doi.org/10.3389/fcimb.2022.894678 |
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author | Jiao, Mengfan Ma, Xiaoxu Li, Yaoguang Wang, Huifen Liu, Ying Guo, Wenhu Lv, Jun |
author_facet | Jiao, Mengfan Ma, Xiaoxu Li, Yaoguang Wang, Huifen Liu, Ying Guo, Wenhu Lv, Jun |
author_sort | Jiao, Mengfan |
collection | PubMed |
description | Nocardia is an opportunistic pathogen that mainly involves immunosuppressed patients and causes a high mortality rate. As an emerging approach to detect infectious pathogens, metagenomic next-generation sequencing (mNGS) was reported in the detection of Nocardia. However, there is no evidence demonstrating the effect of mNGS on the prognosis of Nocardia infection. In this retrospective study, we included 18 nocardiosis patients. Nocardia species were detected by mNGS from their clinical samples. All the patients were diagnosed with nocardiosis by clinical experts through a comprehensive evaluation. Of these 18 patients, fever is the most frequent initial symptom. Compared to traditional culture methods, mNGS provides a faster turnaround time (TAT) and higher sensitivity. Pulmonary nocardiosis was the most common clinical presentation in the study. mNGS detected 13 types of Nocardia species, of which Nocardia abscessus and Nocardia cyriacigeorgica were the most common species. The study’s most noteworthy discovery is that mNGS outperforms culture at detecting mixed infections (more than one pathogen detected in one clinical specimen, including bacteria, fungi, and excluding virus), and number of infectious species was an independent risk factor for nocardiosis patients’ prognostics after adjusting age, ICU days, gender and underlying diseases (adjusted HR = 1.47, 95% CI: 1.09-1.98, p = 0.011). As a result, we believe that by detecting mixed infections (more than one pathogenic species), mNGS can provide a clinical risk warning for the prognosis of nocardiosis. |
format | Online Article Text |
id | pubmed-9471186 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94711862022-09-15 Metagenomic next-generation sequencing provides prognostic warning by identifying mixed infections in nocardiosis Jiao, Mengfan Ma, Xiaoxu Li, Yaoguang Wang, Huifen Liu, Ying Guo, Wenhu Lv, Jun Front Cell Infect Microbiol Cellular and Infection Microbiology Nocardia is an opportunistic pathogen that mainly involves immunosuppressed patients and causes a high mortality rate. As an emerging approach to detect infectious pathogens, metagenomic next-generation sequencing (mNGS) was reported in the detection of Nocardia. However, there is no evidence demonstrating the effect of mNGS on the prognosis of Nocardia infection. In this retrospective study, we included 18 nocardiosis patients. Nocardia species were detected by mNGS from their clinical samples. All the patients were diagnosed with nocardiosis by clinical experts through a comprehensive evaluation. Of these 18 patients, fever is the most frequent initial symptom. Compared to traditional culture methods, mNGS provides a faster turnaround time (TAT) and higher sensitivity. Pulmonary nocardiosis was the most common clinical presentation in the study. mNGS detected 13 types of Nocardia species, of which Nocardia abscessus and Nocardia cyriacigeorgica were the most common species. The study’s most noteworthy discovery is that mNGS outperforms culture at detecting mixed infections (more than one pathogen detected in one clinical specimen, including bacteria, fungi, and excluding virus), and number of infectious species was an independent risk factor for nocardiosis patients’ prognostics after adjusting age, ICU days, gender and underlying diseases (adjusted HR = 1.47, 95% CI: 1.09-1.98, p = 0.011). As a result, we believe that by detecting mixed infections (more than one pathogenic species), mNGS can provide a clinical risk warning for the prognosis of nocardiosis. Frontiers Media S.A. 2022-08-31 /pmc/articles/PMC9471186/ /pubmed/36118026 http://dx.doi.org/10.3389/fcimb.2022.894678 Text en Copyright © 2022 Jiao, Ma, Li, Wang, Liu, Guo and Lv https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Jiao, Mengfan Ma, Xiaoxu Li, Yaoguang Wang, Huifen Liu, Ying Guo, Wenhu Lv, Jun Metagenomic next-generation sequencing provides prognostic warning by identifying mixed infections in nocardiosis |
title | Metagenomic next-generation sequencing provides prognostic warning by identifying mixed infections in nocardiosis |
title_full | Metagenomic next-generation sequencing provides prognostic warning by identifying mixed infections in nocardiosis |
title_fullStr | Metagenomic next-generation sequencing provides prognostic warning by identifying mixed infections in nocardiosis |
title_full_unstemmed | Metagenomic next-generation sequencing provides prognostic warning by identifying mixed infections in nocardiosis |
title_short | Metagenomic next-generation sequencing provides prognostic warning by identifying mixed infections in nocardiosis |
title_sort | metagenomic next-generation sequencing provides prognostic warning by identifying mixed infections in nocardiosis |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471186/ https://www.ncbi.nlm.nih.gov/pubmed/36118026 http://dx.doi.org/10.3389/fcimb.2022.894678 |
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