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Puffball spores improve wound healing in a diabetic rat model

Persistent chronic oxidative stress is a primary pathogenic characteristics of diabetic foot ulcers. Puffball spores are a traditional Chinese medicine used to treat diabetic foot ulcers infections and bedsores. However, their effects against diabetic wounds and the mechanism underlying these effect...

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Autores principales: He, Tangtang, Sun, Pengfei, Liu, Bo, Wan, Shiwei, Fang, Penghua, Chen, Jun, Huang, Guicheng, Min, Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471372/
https://www.ncbi.nlm.nih.gov/pubmed/36120443
http://dx.doi.org/10.3389/fendo.2022.942549
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author He, Tangtang
Sun, Pengfei
Liu, Bo
Wan, Shiwei
Fang, Penghua
Chen, Jun
Huang, Guicheng
Min, Wen
author_facet He, Tangtang
Sun, Pengfei
Liu, Bo
Wan, Shiwei
Fang, Penghua
Chen, Jun
Huang, Guicheng
Min, Wen
author_sort He, Tangtang
collection PubMed
description Persistent chronic oxidative stress is a primary pathogenic characteristics of diabetic foot ulcers. Puffball spores are a traditional Chinese medicine used to treat diabetic foot ulcers infections and bedsores. However, their effects against diabetic wounds and the mechanism underlying these effects remain largely unknown. The present study explored the effectiveness of puffball spores in diabetic wound treatment and the mechanisms underlying their effects. Sprague-Dawley rats with streptozotocin (STZ)-induced diabetes were treated with puffball spores to ascertain whether they accelerated wound healing.Real-time quantitative PCR, western blotting, hematoxylin-eosin and Masson’s trichrome staining, immunohistochemistry analysis, and immunofluorescence assays were performed. As indicated by wound and serum histology and biochemical analyses, the puffball spores accelerated wound healing by activating Akt/Nrf2 signaling and promoting the expression of its downstream antioxidant genes, markedly stimulating antioxidant activity and enhanceing angiogenesis and collagen deposition. Our findings showed that puffball spores could accelerate diabetic wound healing, enhance antioxidant ability, promote the expression of vascular markers, and suppress inflammation, thus providing a theoretical basis for the treatment of diabetic and refractory wounds.
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spelling pubmed-94713722022-09-15 Puffball spores improve wound healing in a diabetic rat model He, Tangtang Sun, Pengfei Liu, Bo Wan, Shiwei Fang, Penghua Chen, Jun Huang, Guicheng Min, Wen Front Endocrinol (Lausanne) Endocrinology Persistent chronic oxidative stress is a primary pathogenic characteristics of diabetic foot ulcers. Puffball spores are a traditional Chinese medicine used to treat diabetic foot ulcers infections and bedsores. However, their effects against diabetic wounds and the mechanism underlying these effects remain largely unknown. The present study explored the effectiveness of puffball spores in diabetic wound treatment and the mechanisms underlying their effects. Sprague-Dawley rats with streptozotocin (STZ)-induced diabetes were treated with puffball spores to ascertain whether they accelerated wound healing.Real-time quantitative PCR, western blotting, hematoxylin-eosin and Masson’s trichrome staining, immunohistochemistry analysis, and immunofluorescence assays were performed. As indicated by wound and serum histology and biochemical analyses, the puffball spores accelerated wound healing by activating Akt/Nrf2 signaling and promoting the expression of its downstream antioxidant genes, markedly stimulating antioxidant activity and enhanceing angiogenesis and collagen deposition. Our findings showed that puffball spores could accelerate diabetic wound healing, enhance antioxidant ability, promote the expression of vascular markers, and suppress inflammation, thus providing a theoretical basis for the treatment of diabetic and refractory wounds. Frontiers Media S.A. 2022-08-31 /pmc/articles/PMC9471372/ /pubmed/36120443 http://dx.doi.org/10.3389/fendo.2022.942549 Text en Copyright © 2022 He, Sun, Liu, Wan, Fang, Chen, Huang and Min https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
He, Tangtang
Sun, Pengfei
Liu, Bo
Wan, Shiwei
Fang, Penghua
Chen, Jun
Huang, Guicheng
Min, Wen
Puffball spores improve wound healing in a diabetic rat model
title Puffball spores improve wound healing in a diabetic rat model
title_full Puffball spores improve wound healing in a diabetic rat model
title_fullStr Puffball spores improve wound healing in a diabetic rat model
title_full_unstemmed Puffball spores improve wound healing in a diabetic rat model
title_short Puffball spores improve wound healing in a diabetic rat model
title_sort puffball spores improve wound healing in a diabetic rat model
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471372/
https://www.ncbi.nlm.nih.gov/pubmed/36120443
http://dx.doi.org/10.3389/fendo.2022.942549
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