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Profiling the serum proteome during Schistosoma mansoni infection in the BALB/c mice: A focus on the altered lipid metabolism as a key modulator of host-parasite interactions

Schistosomiasis represents a condition in which every aspect of the disease, starting from skin invasion of the cercariae to egg laying by adult worms, incites a tissue response from the vertebrate host. This response, whether acute or chronic, leads to the appearance of reporter molecules of tissue...

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Autores principales: Gonçalves-Silva, Gustavo, Vieira, Lara Geralda Magela dos Santos, Cosenza-Contreras, Miguel, Souza, Ana Flávia Pinho, Costa, Daniela Caldeira, Castro-Borges, Wiliam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471378/
https://www.ncbi.nlm.nih.gov/pubmed/36119112
http://dx.doi.org/10.3389/fimmu.2022.955049
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author Gonçalves-Silva, Gustavo
Vieira, Lara Geralda Magela dos Santos
Cosenza-Contreras, Miguel
Souza, Ana Flávia Pinho
Costa, Daniela Caldeira
Castro-Borges, Wiliam
author_facet Gonçalves-Silva, Gustavo
Vieira, Lara Geralda Magela dos Santos
Cosenza-Contreras, Miguel
Souza, Ana Flávia Pinho
Costa, Daniela Caldeira
Castro-Borges, Wiliam
author_sort Gonçalves-Silva, Gustavo
collection PubMed
description Schistosomiasis represents a condition in which every aspect of the disease, starting from skin invasion of the cercariae to egg laying by adult worms, incites a tissue response from the vertebrate host. This response, whether acute or chronic, leads to the appearance of reporter molecules of tissue injury in bodily fluids that could be surveyed as markers for disease diagnosis, status and prognosis. In this scenario, the serum proteome associated with a schistosome infection remains poorly explored; particularly by the use of high-throughput mass spectrometric instrumentation. In this study, we aimed to comparatively examine the serum proteome of control versus infected BALB/c mice, spanning the interval between the onset of egg laying and the peak of the acute phase of infection. Compositional analysis of the sera, using one dimensional reversed-phase fractionation of tryptic peptides coupled to mass spectrometry, allowed identification of 453 constituents. Among these, over 30% (143 molecules) were differentially present comparing sera from infected and non-infected mice, as revealed by quantitative label-free shotgun approach. The majority of proteins exhibiting altered levels was categorised as belonging to immune response (acute phase-related proteins) followed by those linked to lipid transport and metabolism. Inspection of the lipid profile from control and infected individuals demonstrated more pronounced and significant alterations in triglycerides, VLDL and HDL fractions (p<0,001), attesting for a disturbance in circulating lipid molecules, and suggesting a key role in host-parasite interactions. Our findings provide a global view of the serum proteome in the context of experimental schistosomiasis during the acute phase of infection. It contributes by listing key molecules that could be monitored to inform on the associated inflammatory disease status. We hope it will shed light into uncovered aspects of the Schistosoma mansoni parasitism in the vertebrate host, particularly those related to modulation of the lipid metabolism mediating immune responses.
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spelling pubmed-94713782022-09-15 Profiling the serum proteome during Schistosoma mansoni infection in the BALB/c mice: A focus on the altered lipid metabolism as a key modulator of host-parasite interactions Gonçalves-Silva, Gustavo Vieira, Lara Geralda Magela dos Santos Cosenza-Contreras, Miguel Souza, Ana Flávia Pinho Costa, Daniela Caldeira Castro-Borges, Wiliam Front Immunol Immunology Schistosomiasis represents a condition in which every aspect of the disease, starting from skin invasion of the cercariae to egg laying by adult worms, incites a tissue response from the vertebrate host. This response, whether acute or chronic, leads to the appearance of reporter molecules of tissue injury in bodily fluids that could be surveyed as markers for disease diagnosis, status and prognosis. In this scenario, the serum proteome associated with a schistosome infection remains poorly explored; particularly by the use of high-throughput mass spectrometric instrumentation. In this study, we aimed to comparatively examine the serum proteome of control versus infected BALB/c mice, spanning the interval between the onset of egg laying and the peak of the acute phase of infection. Compositional analysis of the sera, using one dimensional reversed-phase fractionation of tryptic peptides coupled to mass spectrometry, allowed identification of 453 constituents. Among these, over 30% (143 molecules) were differentially present comparing sera from infected and non-infected mice, as revealed by quantitative label-free shotgun approach. The majority of proteins exhibiting altered levels was categorised as belonging to immune response (acute phase-related proteins) followed by those linked to lipid transport and metabolism. Inspection of the lipid profile from control and infected individuals demonstrated more pronounced and significant alterations in triglycerides, VLDL and HDL fractions (p<0,001), attesting for a disturbance in circulating lipid molecules, and suggesting a key role in host-parasite interactions. Our findings provide a global view of the serum proteome in the context of experimental schistosomiasis during the acute phase of infection. It contributes by listing key molecules that could be monitored to inform on the associated inflammatory disease status. We hope it will shed light into uncovered aspects of the Schistosoma mansoni parasitism in the vertebrate host, particularly those related to modulation of the lipid metabolism mediating immune responses. Frontiers Media S.A. 2022-08-31 /pmc/articles/PMC9471378/ /pubmed/36119112 http://dx.doi.org/10.3389/fimmu.2022.955049 Text en Copyright © 2022 Gonçalves-Silva, Vieira, Cosenza-Contreras, Souza, Costa and Castro-Borges https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Gonçalves-Silva, Gustavo
Vieira, Lara Geralda Magela dos Santos
Cosenza-Contreras, Miguel
Souza, Ana Flávia Pinho
Costa, Daniela Caldeira
Castro-Borges, Wiliam
Profiling the serum proteome during Schistosoma mansoni infection in the BALB/c mice: A focus on the altered lipid metabolism as a key modulator of host-parasite interactions
title Profiling the serum proteome during Schistosoma mansoni infection in the BALB/c mice: A focus on the altered lipid metabolism as a key modulator of host-parasite interactions
title_full Profiling the serum proteome during Schistosoma mansoni infection in the BALB/c mice: A focus on the altered lipid metabolism as a key modulator of host-parasite interactions
title_fullStr Profiling the serum proteome during Schistosoma mansoni infection in the BALB/c mice: A focus on the altered lipid metabolism as a key modulator of host-parasite interactions
title_full_unstemmed Profiling the serum proteome during Schistosoma mansoni infection in the BALB/c mice: A focus on the altered lipid metabolism as a key modulator of host-parasite interactions
title_short Profiling the serum proteome during Schistosoma mansoni infection in the BALB/c mice: A focus on the altered lipid metabolism as a key modulator of host-parasite interactions
title_sort profiling the serum proteome during schistosoma mansoni infection in the balb/c mice: a focus on the altered lipid metabolism as a key modulator of host-parasite interactions
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471378/
https://www.ncbi.nlm.nih.gov/pubmed/36119112
http://dx.doi.org/10.3389/fimmu.2022.955049
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