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Anti-stress Effect of Octopus Cephalotocin in Rats

Cephalotocin is a bioactivity-regulating peptide expressed in octopus (Octopus vulgaris). The peptide sequence of cephalotocin is very similar to the peptide sequence of mammalian vasopressin, and cephalotocin has been proposed to mainly activate arginine vasopressin 1b receptor (Avpr1b) in the brai...

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Autores principales: Kim, Ye-Ji, Jo, Seonmi, Jung, Seung-Hyun, Woo, Dong Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Brain and Neural Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471412/
https://www.ncbi.nlm.nih.gov/pubmed/36050225
http://dx.doi.org/10.5607/en22010
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author Kim, Ye-Ji
Jo, Seonmi
Jung, Seung-Hyun
Woo, Dong Ho
author_facet Kim, Ye-Ji
Jo, Seonmi
Jung, Seung-Hyun
Woo, Dong Ho
author_sort Kim, Ye-Ji
collection PubMed
description Cephalotocin is a bioactivity-regulating peptide expressed in octopus (Octopus vulgaris). The peptide sequence of cephalotocin is very similar to the peptide sequence of mammalian vasopressin, and cephalotocin has been proposed to mainly activate arginine vasopressin 1b receptor (Avpr1b) in the brain. However, the effects of cephalotocin on mammalian behavior have not been studied. In the current study, cephalotocin significantly reduced both the frequency and amplitude of spontaneous excitatory postsynaptic currents (sEPSCs) recorded from not only cultured neuronal cells from postnatal Sprague–Dawley (SD) rats but also hippocampal slices from 4-week-old male C57BL/6 mice. Intraperitoneal (IP) injection did not affect the open field behaviors of C57BL/6 mice. Cephalotocin was directly infused into the hippocampus because the normalized Avpr1b staining intensity divided by the DAPI staining intensity indicated that Avpr1b expression tended to be high in the hippocampus. A hippocampal infusion of 1 mg/kg cephalotocin via an implanted cannula exerted an anti-stress effect, significantly reducing the immobility time in the tail suspension test (TST). The present results provide evidence that the effects of cephalotocin on the activity of hippocampal neurons are related to ameliorating stress, suggesting that cephalotocin may be developed as an anti-stress biomodulator that functions by affecting the brain.
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spelling pubmed-94714122022-09-19 Anti-stress Effect of Octopus Cephalotocin in Rats Kim, Ye-Ji Jo, Seonmi Jung, Seung-Hyun Woo, Dong Ho Exp Neurobiol Original Article Cephalotocin is a bioactivity-regulating peptide expressed in octopus (Octopus vulgaris). The peptide sequence of cephalotocin is very similar to the peptide sequence of mammalian vasopressin, and cephalotocin has been proposed to mainly activate arginine vasopressin 1b receptor (Avpr1b) in the brain. However, the effects of cephalotocin on mammalian behavior have not been studied. In the current study, cephalotocin significantly reduced both the frequency and amplitude of spontaneous excitatory postsynaptic currents (sEPSCs) recorded from not only cultured neuronal cells from postnatal Sprague–Dawley (SD) rats but also hippocampal slices from 4-week-old male C57BL/6 mice. Intraperitoneal (IP) injection did not affect the open field behaviors of C57BL/6 mice. Cephalotocin was directly infused into the hippocampus because the normalized Avpr1b staining intensity divided by the DAPI staining intensity indicated that Avpr1b expression tended to be high in the hippocampus. A hippocampal infusion of 1 mg/kg cephalotocin via an implanted cannula exerted an anti-stress effect, significantly reducing the immobility time in the tail suspension test (TST). The present results provide evidence that the effects of cephalotocin on the activity of hippocampal neurons are related to ameliorating stress, suggesting that cephalotocin may be developed as an anti-stress biomodulator that functions by affecting the brain. The Korean Society for Brain and Neural Sciences 2022-08-31 2022-08-31 /pmc/articles/PMC9471412/ /pubmed/36050225 http://dx.doi.org/10.5607/en22010 Text en Copyright © Experimental Neurobiology 2022 https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Ye-Ji
Jo, Seonmi
Jung, Seung-Hyun
Woo, Dong Ho
Anti-stress Effect of Octopus Cephalotocin in Rats
title Anti-stress Effect of Octopus Cephalotocin in Rats
title_full Anti-stress Effect of Octopus Cephalotocin in Rats
title_fullStr Anti-stress Effect of Octopus Cephalotocin in Rats
title_full_unstemmed Anti-stress Effect of Octopus Cephalotocin in Rats
title_short Anti-stress Effect of Octopus Cephalotocin in Rats
title_sort anti-stress effect of octopus cephalotocin in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471412/
https://www.ncbi.nlm.nih.gov/pubmed/36050225
http://dx.doi.org/10.5607/en22010
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