Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC)

Different types of immune cells are involved in atherogenesis and may act atheroprotective or atheroprogressive. Here, we describe an in vitro approach to analyze CD11c(+) cells and CD11c(+)-derived ApoE in atherosclerosis. The major steps include harvesting mouse bone marrow, plating cells in cultu...

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Autores principales: Sauter, Manuela, Sauter, Reinhard J., Nording, Henry, Olbrich, Marcus, Emschermann, Frederic, Langer, Harald F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471451/
https://www.ncbi.nlm.nih.gov/pubmed/36097382
http://dx.doi.org/10.1016/j.xpro.2022.101664
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author Sauter, Manuela
Sauter, Reinhard J.
Nording, Henry
Olbrich, Marcus
Emschermann, Frederic
Langer, Harald F.
author_facet Sauter, Manuela
Sauter, Reinhard J.
Nording, Henry
Olbrich, Marcus
Emschermann, Frederic
Langer, Harald F.
author_sort Sauter, Manuela
collection PubMed
description Different types of immune cells are involved in atherogenesis and may act atheroprotective or atheroprogressive. Here, we describe an in vitro approach to analyze CD11c(+) cells and CD11c(+)-derived ApoE in atherosclerosis. The major steps include harvesting mouse bone marrow, plating cells in culture dishes, treating them with differentiation factors, and collecting cells after removal of undesirable populations. This protocol can be adapted for CD11c(+) cells in different contexts, thus, serving as models for different diseases and to analyze cell-specific molecules. For complete details on the use and execution of this protocol, please refer to Sauter et al. (2021).
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spelling pubmed-94714512022-09-15 Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC) Sauter, Manuela Sauter, Reinhard J. Nording, Henry Olbrich, Marcus Emschermann, Frederic Langer, Harald F. STAR Protoc Protocol Different types of immune cells are involved in atherogenesis and may act atheroprotective or atheroprogressive. Here, we describe an in vitro approach to analyze CD11c(+) cells and CD11c(+)-derived ApoE in atherosclerosis. The major steps include harvesting mouse bone marrow, plating cells in culture dishes, treating them with differentiation factors, and collecting cells after removal of undesirable populations. This protocol can be adapted for CD11c(+) cells in different contexts, thus, serving as models for different diseases and to analyze cell-specific molecules. For complete details on the use and execution of this protocol, please refer to Sauter et al. (2021). Elsevier 2022-09-08 /pmc/articles/PMC9471451/ /pubmed/36097382 http://dx.doi.org/10.1016/j.xpro.2022.101664 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sauter, Manuela
Sauter, Reinhard J.
Nording, Henry
Olbrich, Marcus
Emschermann, Frederic
Langer, Harald F.
Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC)
title Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC)
title_full Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC)
title_fullStr Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC)
title_full_unstemmed Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC)
title_short Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC)
title_sort protocol to isolate and analyze mouse bone marrow derived dendritic cells (bmdc)
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471451/
https://www.ncbi.nlm.nih.gov/pubmed/36097382
http://dx.doi.org/10.1016/j.xpro.2022.101664
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