Cargando…

Protocol to isolate and analyze mouse bone marrow derived dendritic cells (BMDC)

Different types of immune cells are involved in atherogenesis and may act atheroprotective or atheroprogressive. Here, we describe an in vitro approach to analyze CD11c(+) cells and CD11c(+)-derived ApoE in atherosclerosis. The major steps include harvesting mouse bone marrow, plating cells in cultu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Sauter, Manuela, Sauter, Reinhard J., Nording, Henry, Olbrich, Marcus, Emschermann, Frederic, Langer, Harald F.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471451/ https://www.ncbi.nlm.nih.gov/pubmed/36097382 http://dx.doi.org/10.1016/j.xpro.2022.101664

Ejemplares similares

Protocol to visualize CD11c(+) cells in atherosclerosis using LacZ reporter mice
por: Sauter, Manuela, et al.
Publicado: (2022)

Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer
por: Gotoh, Kazuhito, et al.
Publicado: (2021)

Apolipoprotein E derived from CD11c(+) cells ameliorates atherosclerosis
por: Sauter, Manuela, et al.
Publicado: (2021)

Platelets and Immune Responses During Thromboinflammation
por: Mezger, Matthias, et al.
Publicado: (2019)

The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets
por: Nording, Henry, et al.
Publicado: (2021)

Gene Set Enrichment Analysis Reveals That Fucoidan Induces Type I IFN Pathways in BMDC
por: Choi, Suyoung, et al.
Publicado: (2022)

Biomedical Data Commons (BMDC) prioritizes B-lymphocyte non-coding genetic variants in Type 1 Diabetes
por: Piekos, Samantha N., et al.
Publicado: (2021)

A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution
por: Jacquelot, Nicolas, et al.
Publicado: (2022)

Conventional echocardiographic parameters or three-dimensional echocardiography to evaluate right ventricular function in percutaneous edge-to-edge mitral valve repair (PMVR)
por: Sauter, Reinhard J., et al.
Publicado: (2019)

Isolation of human bone marrow stromal cells from bone marrow biopsies for single-cell RNA sequencing
por: Gleitz, Hélène F.E., et al.
Publicado: (2021)

Protocol to produce high-titer retrovirus for transduction of mouse bone marrow cells
por: Qu, Jing, et al.
Publicado: (2021)

Assessing mRNA translation in mouse adult microglia and bone-marrow-derived macrophages
por: Antignano, Ignazio, et al.
Publicado: (2023)

Protocol for live imaging of transferred mouse bone marrow cells by two-photon microscopy
por: Sudo, Takao, et al.
Publicado: (2023)

A protocol for human bone marrow adipocyte isolation and purification
por: Attané, Camille, et al.
Publicado: (2021)

Platelets in Inflammation and Atherogenesis
por: Nording, Henry M., et al.
Publicado: (2015)

Platelets as Mediators of Neuroinflammation and Thrombosis
por: Rawish, Elias, et al.
Publicado: (2020)

Analyzing autophagosomes and mitophagosomes in the mouse brain using electron microscopy
por: Duan, Kaizheng, et al.
Publicado: (2022)

Labeling and analyzing lipid droplets in mouse muscle stem cells
por: Chen, Jingjuan, et al.
Publicado: (2022)

Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromes
por: Ganan-Gomez, Irene, et al.
Publicado: (2022)

Inflammation in Metabolic and Cardiovascular Disorders—Role of Oxidative Stress
por: Sun, Ying, et al.
Publicado: (2021)

Use of Busulfan to Condition Mice for Bone Marrow Transplantation
por: Montecino-Rodriguez, Encarnacion, et al.
Publicado: (2020)

Protocol for performing consecutive bone marrow transplants in mice to study the role of marrow niche in supporting hematopoiesis
por: Gopal, Aparna, et al.
Publicado: (2023)

Flow-cytometry-based protocol to analyze respiratory chain function in mouse microglia
por: Erny, Daniel, et al.
Publicado: (2022)

Mass cytometry staining for human bone marrow clinical samples
por: Hallisey, Margaret, et al.
Publicado: (2022)

Effect of Matrix Metallopeptidase 13 on the Function of Mouse Bone Marrow-derived Dendritic Cells
por: Li, Xiao-Dong, et al.
Publicado: (2017)

Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy
por: Nakai-Shimoda, Hiromi, et al.
Publicado: (2022)

Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human
por: Maiques, Oscar, et al.
Publicado: (2022)

Elevated Mitral Valve Pressure Gradient Is Predictive of Long‐Term Outcome After Percutaneous Edge‐to‐Edge Mitral Valve Repair in Patients With Degenerative Mitral Regurgitation (MR), But Not in Functional MR
por: Patzelt, Johannes, et al.
Publicado: (2019)

Novel Protocol for the Preparation of Porcine Bone Marrow Primary Cell Culture for African Swine Fever Virus Isolation
por: Puzankova, Olga, et al.
Publicado: (2023)

How to extract and analyze pollen from internal organs and exoskeletons of fossil insects?
por: Grímsson, Friðgeir, et al.
Publicado: (2021)

Culture and Identification of Mouse Bone Marrow-Derived Dendritic Cells and Their Capability to Induce T Lymphocyte Proliferation
por: Wang, Wenguang, et al.
Publicado: (2016)

Structure and conductivity of ionomer in PEM fuel cell catalyst layers: a model-based analysis
por: Olbrich, W., et al.
Publicado: (2023)

scFASTCORMICS: A Contextualization Algorithm to Reconstruct Metabolic Multi-Cell Population Models from Single-Cell RNAseq Data
por: Pacheco, Maria Pires, et al.
Publicado: (2022)

Preparation and culture of bone marrow-derived macrophages from mice for functional analysis
por: Toda, Gotaro, et al.
Publicado: (2020)

DIA label-free proteomic analysis of murine bone-marrow-derived macrophages
por: Baker, Christa P., et al.
Publicado: (2022)

Presence of bone marrow micro‐metastases in stage I‐III colon cancer patients is associated with worse disease‐free and overall survival
por: Viehl, Carsten T., et al.
Publicado: (2017)

Discrimination of the heterogeneity of bone marrow-derived dendritic cells
por: Wang, Jing, et al.
Publicado: (2017)

Conceptualizing Interprofessional Digital Communication and Collaboration in Health Care: Protocol for a Scoping Review
por: Nordmann, Kim, et al.
Publicado: (2023)

In vitro and ex vivo evaluation of tumor-derived exosome-induced dendritic cell dysfunction in mouse
por: Zeng, Wenfeng, et al.
Publicado: (2021)

A quantitative assessment of the content of hematopoietic stem cells in mouse and human endosteal-bone marrow: a simple and rapid method for the isolation of mouse central bone marrow
por: Mahajan, Maya M., et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_qf01sln3t6omkrk1l9n1g81mbg