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A biochemical reconstitution approach to identify autophagy receptors for aggrephagy in mammalian cells
Aggrephagy is a major way to clear protein aggregates. Here, we describe a pipeline of experiments to find autophagy receptors for aggrephagy. Steps include an in vitro reconstitution to recapitulate autophagosome recognizing aggregates and receptor identification steps based on flow cytometry and m...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471455/ https://www.ncbi.nlm.nih.gov/pubmed/36097383 http://dx.doi.org/10.1016/j.xpro.2022.101662 |