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A biochemical reconstitution approach to identify autophagy receptors for aggrephagy in mammalian cells
Aggrephagy is a major way to clear protein aggregates. Here, we describe a pipeline of experiments to find autophagy receptors for aggrephagy. Steps include an in vitro reconstitution to recapitulate autophagosome recognizing aggregates and receptor identification steps based on flow cytometry and m...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471455/ https://www.ncbi.nlm.nih.gov/pubmed/36097383 http://dx.doi.org/10.1016/j.xpro.2022.101662 |