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Co-culture of primary human T cells with leukemia cells to measure regulatory T cell expansion

The expansion of regulatory T cells (Tregs) is known to be mediated by cytokines including IL-10 and TGFβ but has additionally been shown to depend on the interaction of the immune receptors ICOSLG and ICOS. Here, we describe a co-culture system which enables quantification of the ability of leukemi...

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Detalles Bibliográficos
Autores principales: Külp, Marius, Diehl, Laura, Bonig, Halvard, Marschalek, Rolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9471457/
https://www.ncbi.nlm.nih.gov/pubmed/36097388
http://dx.doi.org/10.1016/j.xpro.2022.101661
Descripción
Sumario:The expansion of regulatory T cells (Tregs) is known to be mediated by cytokines including IL-10 and TGFβ but has additionally been shown to depend on the interaction of the immune receptors ICOSLG and ICOS. Here, we describe a co-culture system which enables quantification of the ability of leukemia cells to induce Treg expansion through secreted cytokines and direct receptor interactions. The protocol is applicable for MHC-matched and -unmatched experiments and allows assessment of Treg expansion without using a mouse model. For complete details on the use and execution of this protocol, please refer to Külp et al. (2022).