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Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery

The tea plant is a kind of ammonium-preferring crop, but the mechanism whereby ammonium (NH(4)(+)) regulate its growth is not well understood. The current study focused on the effects of NH(4)(+) on tea plants. Transcriptomic analysis was performed to investigate the early- and late-stage NH(4)(+) d...

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Autores principales: Wang, Yu, Ouyang, Jia-Xue, Fan, Dong-Mei, Wang, Shu-Mao, Xuan, Yi-Min, Wang, Xiao-Chang, Zheng, Xin-Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9472221/
https://www.ncbi.nlm.nih.gov/pubmed/36119592
http://dx.doi.org/10.3389/fpls.2022.963269
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author Wang, Yu
Ouyang, Jia-Xue
Fan, Dong-Mei
Wang, Shu-Mao
Xuan, Yi-Min
Wang, Xiao-Chang
Zheng, Xin-Qiang
author_facet Wang, Yu
Ouyang, Jia-Xue
Fan, Dong-Mei
Wang, Shu-Mao
Xuan, Yi-Min
Wang, Xiao-Chang
Zheng, Xin-Qiang
author_sort Wang, Yu
collection PubMed
description The tea plant is a kind of ammonium-preferring crop, but the mechanism whereby ammonium (NH(4)(+)) regulate its growth is not well understood. The current study focused on the effects of NH(4)(+) on tea plants. Transcriptomic analysis was performed to investigate the early- and late-stage NH(4)(+) deprivation and resupply in tea plants shoots. Through short- and long-term NH(4)(+) deficiency, the dynamic response to NH(4)(+) stress was investigated. The most significant effects of NH(4)(+) deficiency were found to be on photosynthesis and gene ontology (GO) enrichment varied with the length of NH(4)(+) deprivation. Enriched KEGG pathways were also different when NH(4)(+) was resupplied at different concentrations which may indicate reasons for tolerance of high NH(4)(+) concentration. Using weighted gene co-expression network analysis (WGCNA), modules related to significant tea components, tea polyphenols and free amino acids, were identified. Hence, NH(4)(+) could be regarded as a signaling molecule with the response of catechins shown to be higher than that of amino acids. The current work represents a comprehensive transcriptomic analysis of plant responses to NH(4)(+) and reveals many potential genes regulated by NH(4)(+) in tea plants. Such findings may lead to improvements in nitrogen efficiency of tea plants.
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spelling pubmed-94722212022-09-15 Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery Wang, Yu Ouyang, Jia-Xue Fan, Dong-Mei Wang, Shu-Mao Xuan, Yi-Min Wang, Xiao-Chang Zheng, Xin-Qiang Front Plant Sci Plant Science The tea plant is a kind of ammonium-preferring crop, but the mechanism whereby ammonium (NH(4)(+)) regulate its growth is not well understood. The current study focused on the effects of NH(4)(+) on tea plants. Transcriptomic analysis was performed to investigate the early- and late-stage NH(4)(+) deprivation and resupply in tea plants shoots. Through short- and long-term NH(4)(+) deficiency, the dynamic response to NH(4)(+) stress was investigated. The most significant effects of NH(4)(+) deficiency were found to be on photosynthesis and gene ontology (GO) enrichment varied with the length of NH(4)(+) deprivation. Enriched KEGG pathways were also different when NH(4)(+) was resupplied at different concentrations which may indicate reasons for tolerance of high NH(4)(+) concentration. Using weighted gene co-expression network analysis (WGCNA), modules related to significant tea components, tea polyphenols and free amino acids, were identified. Hence, NH(4)(+) could be regarded as a signaling molecule with the response of catechins shown to be higher than that of amino acids. The current work represents a comprehensive transcriptomic analysis of plant responses to NH(4)(+) and reveals many potential genes regulated by NH(4)(+) in tea plants. Such findings may lead to improvements in nitrogen efficiency of tea plants. Frontiers Media S.A. 2022-08-30 /pmc/articles/PMC9472221/ /pubmed/36119592 http://dx.doi.org/10.3389/fpls.2022.963269 Text en Copyright © 2022 Wang, Ouyang, Fan, Wang, Xuan, Wang and Zheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Yu
Ouyang, Jia-Xue
Fan, Dong-Mei
Wang, Shu-Mao
Xuan, Yi-Min
Wang, Xiao-Chang
Zheng, Xin-Qiang
Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery
title Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery
title_full Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery
title_fullStr Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery
title_full_unstemmed Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery
title_short Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery
title_sort transcriptome analysis of tea (camellia sinensis) leaves in response to ammonium starvation and recovery
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9472221/
https://www.ncbi.nlm.nih.gov/pubmed/36119592
http://dx.doi.org/10.3389/fpls.2022.963269
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