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Microbial contamination, antimicrobial resistance and biofilm formation of bacteria isolated from a high-throughput pig abattoir

The aim of this work was to assess the level of microbial contamination and resistance of bacteria isolated from a highthroughput heavy pig slaughterhouse (approx. 4600 pigs/day) towards antimicrobials considered as critical for human, veterinary or both chemotherapies. Samples, pre-operative and op...

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Autores principales: Ghidini, Sergio, De Luca, Silvio, Rodríguez-López, Pedro, Simon, Ancuţa Cezara, Liuzzo, Gaetano, Poli, Luca, Ianieri, Adriana, Zanardi, Emanuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9472283/
https://www.ncbi.nlm.nih.gov/pubmed/36120528
http://dx.doi.org/10.4081/ijfs.2022.10160
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author Ghidini, Sergio
De Luca, Silvio
Rodríguez-López, Pedro
Simon, Ancuţa Cezara
Liuzzo, Gaetano
Poli, Luca
Ianieri, Adriana
Zanardi, Emanuela
author_facet Ghidini, Sergio
De Luca, Silvio
Rodríguez-López, Pedro
Simon, Ancuţa Cezara
Liuzzo, Gaetano
Poli, Luca
Ianieri, Adriana
Zanardi, Emanuela
author_sort Ghidini, Sergio
collection PubMed
description The aim of this work was to assess the level of microbial contamination and resistance of bacteria isolated from a highthroughput heavy pig slaughterhouse (approx. 4600 pigs/day) towards antimicrobials considered as critical for human, veterinary or both chemotherapies. Samples, pre-operative and operative, were obtained in 4 different surveys. These comprised environmental sampling, i.e. air (n(total) = 192) and surfaces (n(total) = 32), in four different locations. Moreover, a total of 40 carcasses were sampled in two different moments of slaughtering following Reg. (CE) 2073/2005. Overall, 60 different colonies were randomly selected from VRBGA plates belonging to 20 species, 15 genera and 10 families being Enterobacteriaceae, Moraxellaceae and Pseudomonadaceae the most represented ones. Thirty-seven isolates presented resistance to at least one molecule and seventeen were classified as multi-drug resistant. Enterobacteriaceae, particularly E. coli, displayed high MIC values towards trimethoprim, ampicillin, tetracycline and sulphametoxazole with MIC(max) of 16, 32, 32 and 512 mg/L, respectively. Moreover, isolated Pseudomonas spp. showed high MIC values in critical antibiotics such as ampicillin and azithromycin with MIC(max) of 32 and 64 mg/L, respectively. Additionally, in vitro biofilm formation assays demonstrated that fifteen of these isolates can be classified as strong biofilm formers. Results demonstrated that a high diversity of bacteria containing antibiotic resistant and multiresistant species is present in the sampled abattoir. Considering these findings, it could be hypothesised that the processing environment could be a potential diffusion determinant of antibiotic resistant bacteria through the food chain and operators.
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spelling pubmed-94722832022-09-15 Microbial contamination, antimicrobial resistance and biofilm formation of bacteria isolated from a high-throughput pig abattoir Ghidini, Sergio De Luca, Silvio Rodríguez-López, Pedro Simon, Ancuţa Cezara Liuzzo, Gaetano Poli, Luca Ianieri, Adriana Zanardi, Emanuela Ital J Food Saf Article The aim of this work was to assess the level of microbial contamination and resistance of bacteria isolated from a highthroughput heavy pig slaughterhouse (approx. 4600 pigs/day) towards antimicrobials considered as critical for human, veterinary or both chemotherapies. Samples, pre-operative and operative, were obtained in 4 different surveys. These comprised environmental sampling, i.e. air (n(total) = 192) and surfaces (n(total) = 32), in four different locations. Moreover, a total of 40 carcasses were sampled in two different moments of slaughtering following Reg. (CE) 2073/2005. Overall, 60 different colonies were randomly selected from VRBGA plates belonging to 20 species, 15 genera and 10 families being Enterobacteriaceae, Moraxellaceae and Pseudomonadaceae the most represented ones. Thirty-seven isolates presented resistance to at least one molecule and seventeen were classified as multi-drug resistant. Enterobacteriaceae, particularly E. coli, displayed high MIC values towards trimethoprim, ampicillin, tetracycline and sulphametoxazole with MIC(max) of 16, 32, 32 and 512 mg/L, respectively. Moreover, isolated Pseudomonas spp. showed high MIC values in critical antibiotics such as ampicillin and azithromycin with MIC(max) of 32 and 64 mg/L, respectively. Additionally, in vitro biofilm formation assays demonstrated that fifteen of these isolates can be classified as strong biofilm formers. Results demonstrated that a high diversity of bacteria containing antibiotic resistant and multiresistant species is present in the sampled abattoir. Considering these findings, it could be hypothesised that the processing environment could be a potential diffusion determinant of antibiotic resistant bacteria through the food chain and operators. PAGEPress Publications, Pavia, Italy 2022-08-11 /pmc/articles/PMC9472283/ /pubmed/36120528 http://dx.doi.org/10.4081/ijfs.2022.10160 Text en ©Copyright: the Author(s) https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Ghidini, Sergio
De Luca, Silvio
Rodríguez-López, Pedro
Simon, Ancuţa Cezara
Liuzzo, Gaetano
Poli, Luca
Ianieri, Adriana
Zanardi, Emanuela
Microbial contamination, antimicrobial resistance and biofilm formation of bacteria isolated from a high-throughput pig abattoir
title Microbial contamination, antimicrobial resistance and biofilm formation of bacteria isolated from a high-throughput pig abattoir
title_full Microbial contamination, antimicrobial resistance and biofilm formation of bacteria isolated from a high-throughput pig abattoir
title_fullStr Microbial contamination, antimicrobial resistance and biofilm formation of bacteria isolated from a high-throughput pig abattoir
title_full_unstemmed Microbial contamination, antimicrobial resistance and biofilm formation of bacteria isolated from a high-throughput pig abattoir
title_short Microbial contamination, antimicrobial resistance and biofilm formation of bacteria isolated from a high-throughput pig abattoir
title_sort microbial contamination, antimicrobial resistance and biofilm formation of bacteria isolated from a high-throughput pig abattoir
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9472283/
https://www.ncbi.nlm.nih.gov/pubmed/36120528
http://dx.doi.org/10.4081/ijfs.2022.10160
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