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Effects of embryo injected with ochratoxins A on hatching quality and jejunum antioxidant capacity of ducks at hatching

Numerous studies have shown that ochratoxins A (OTA) exerts diverse toxicological effects, namely, hepatotoxicity, nephrotoxicity, genotoxicity, enterotoxicity, and immunotoxicity. The main objective of this study was to investigate the influence of embryonic exposure to OTA by different injection t...

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Autores principales: Liu, Jinhui, Jiang, Xiayu, Peng, Xin, Yuan, Yihan, Shen, Yu, Li, Yongxia, Yan, Zixing, Yuan, Xi, Yang, Ye, Zhai, Shuangshuang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9472544/
https://www.ncbi.nlm.nih.gov/pubmed/36118355
http://dx.doi.org/10.3389/fvets.2022.944891
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author Liu, Jinhui
Jiang, Xiayu
Peng, Xin
Yuan, Yihan
Shen, Yu
Li, Yongxia
Yan, Zixing
Yuan, Xi
Yang, Ye
Zhai, Shuangshuang
author_facet Liu, Jinhui
Jiang, Xiayu
Peng, Xin
Yuan, Yihan
Shen, Yu
Li, Yongxia
Yan, Zixing
Yuan, Xi
Yang, Ye
Zhai, Shuangshuang
author_sort Liu, Jinhui
collection PubMed
description Numerous studies have shown that ochratoxins A (OTA) exerts diverse toxicological effects, namely, hepatotoxicity, nephrotoxicity, genotoxicity, enterotoxicity, and immunotoxicity. The main objective of this study was to investigate the influence of embryonic exposure to OTA by different injection times and OTA doses on hatching quality and jejunal antioxidant capacity of ducks at hatching. In total, 480 fertilized eggs were weighed and randomly assigned into a 4 × 4 factorial design including four OTA doses (0, 2, 4, and 8 ng/g egg) on 8, 13, 18, and 23 of embryonic development (E8, E13, E18, and E23). Each treatment included 6 repeats with 5 eggs per repeat. The results showed that the injection time affected the hatching weight (P < 0.0001). The relative length of the jejunum and ileum on E18 and E23 was lower than on E8 and E13 (P < 0.05). Injection time, doses, and their interaction had no effect on jejunum morphology, namely, villous height (Vh), crypt depth (Cd), and villous height/crypt depth ratio Vh/Cd (P > 0.05). The injection time affected the activities of Superoxide dismutase (SOD) (P < 0.0001), total antioxidant capacity (T-AOC) (P < 0.05) and the malondialdehyde (MDA) content (P < 0.0001). The activity of SOD and T-AOC activities in the jejunum of ducklings injected with OTA at the E8 and E13 was lower than that injected at the E18 (P < 0.05). The highest MDA content was observed in ducklings injected with OTA at the E13 (P < 0.05). The injection time (P < 0.0001), OTA doses and their interaction affected the contents of IL-1β (P < 0.05), which significantly increased especially on E13. In conclusion, the embryo injected with ochratoxins A affected the hatching weight, the relative length of jejunum and ileum, decreased the antioxidant capacity and increased the content of proinflammatory cytokine IL-1β of the jejunum.
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spelling pubmed-94725442022-09-15 Effects of embryo injected with ochratoxins A on hatching quality and jejunum antioxidant capacity of ducks at hatching Liu, Jinhui Jiang, Xiayu Peng, Xin Yuan, Yihan Shen, Yu Li, Yongxia Yan, Zixing Yuan, Xi Yang, Ye Zhai, Shuangshuang Front Vet Sci Veterinary Science Numerous studies have shown that ochratoxins A (OTA) exerts diverse toxicological effects, namely, hepatotoxicity, nephrotoxicity, genotoxicity, enterotoxicity, and immunotoxicity. The main objective of this study was to investigate the influence of embryonic exposure to OTA by different injection times and OTA doses on hatching quality and jejunal antioxidant capacity of ducks at hatching. In total, 480 fertilized eggs were weighed and randomly assigned into a 4 × 4 factorial design including four OTA doses (0, 2, 4, and 8 ng/g egg) on 8, 13, 18, and 23 of embryonic development (E8, E13, E18, and E23). Each treatment included 6 repeats with 5 eggs per repeat. The results showed that the injection time affected the hatching weight (P < 0.0001). The relative length of the jejunum and ileum on E18 and E23 was lower than on E8 and E13 (P < 0.05). Injection time, doses, and their interaction had no effect on jejunum morphology, namely, villous height (Vh), crypt depth (Cd), and villous height/crypt depth ratio Vh/Cd (P > 0.05). The injection time affected the activities of Superoxide dismutase (SOD) (P < 0.0001), total antioxidant capacity (T-AOC) (P < 0.05) and the malondialdehyde (MDA) content (P < 0.0001). The activity of SOD and T-AOC activities in the jejunum of ducklings injected with OTA at the E8 and E13 was lower than that injected at the E18 (P < 0.05). The highest MDA content was observed in ducklings injected with OTA at the E13 (P < 0.05). The injection time (P < 0.0001), OTA doses and their interaction affected the contents of IL-1β (P < 0.05), which significantly increased especially on E13. In conclusion, the embryo injected with ochratoxins A affected the hatching weight, the relative length of jejunum and ileum, decreased the antioxidant capacity and increased the content of proinflammatory cytokine IL-1β of the jejunum. Frontiers Media S.A. 2022-08-09 /pmc/articles/PMC9472544/ /pubmed/36118355 http://dx.doi.org/10.3389/fvets.2022.944891 Text en Copyright © 2022 Liu, Jiang, Peng, Yuan, Shen, Li, Yan, Yuan, Yang and Zhai. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Liu, Jinhui
Jiang, Xiayu
Peng, Xin
Yuan, Yihan
Shen, Yu
Li, Yongxia
Yan, Zixing
Yuan, Xi
Yang, Ye
Zhai, Shuangshuang
Effects of embryo injected with ochratoxins A on hatching quality and jejunum antioxidant capacity of ducks at hatching
title Effects of embryo injected with ochratoxins A on hatching quality and jejunum antioxidant capacity of ducks at hatching
title_full Effects of embryo injected with ochratoxins A on hatching quality and jejunum antioxidant capacity of ducks at hatching
title_fullStr Effects of embryo injected with ochratoxins A on hatching quality and jejunum antioxidant capacity of ducks at hatching
title_full_unstemmed Effects of embryo injected with ochratoxins A on hatching quality and jejunum antioxidant capacity of ducks at hatching
title_short Effects of embryo injected with ochratoxins A on hatching quality and jejunum antioxidant capacity of ducks at hatching
title_sort effects of embryo injected with ochratoxins a on hatching quality and jejunum antioxidant capacity of ducks at hatching
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9472544/
https://www.ncbi.nlm.nih.gov/pubmed/36118355
http://dx.doi.org/10.3389/fvets.2022.944891
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