Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion

We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome conce...

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Detalles Bibliográficos
Autores principales: Jumeaux, Coline, Kim, Eunjung, Howes, Philip D., Kim, Hyemin, Chandrawati, Rona, Stevens, Molly M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9473185/
https://www.ncbi.nlm.nih.gov/pubmed/36132259
http://dx.doi.org/10.1039/c8na00331a
Descripción
Sumario:We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome concentration, and assay temperature, we demonstrated a sub-nanomolar limit of detection for the target.

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