Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion

We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome conce...

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Detalles Bibliográficos
Autores principales: Jumeaux, Coline, Kim, Eunjung, Howes, Philip D., Kim, Hyemin, Chandrawati, Rona, Stevens, Molly M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9473185/
https://www.ncbi.nlm.nih.gov/pubmed/36132259
http://dx.doi.org/10.1039/c8na00331a
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author Jumeaux, Coline
Kim, Eunjung
Howes, Philip D.
Kim, Hyemin
Chandrawati, Rona
Stevens, Molly M.
author_facet Jumeaux, Coline
Kim, Eunjung
Howes, Philip D.
Kim, Hyemin
Chandrawati, Rona
Stevens, Molly M.
author_sort Jumeaux, Coline
collection PubMed
description We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome concentration, and assay temperature, we demonstrated a sub-nanomolar limit of detection for the target.
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spelling pubmed-94731852022-09-20 Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion Jumeaux, Coline Kim, Eunjung Howes, Philip D. Kim, Hyemin Chandrawati, Rona Stevens, Molly M. Nanoscale Adv Chemistry We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome concentration, and assay temperature, we demonstrated a sub-nanomolar limit of detection for the target. RSC 2018-11-21 /pmc/articles/PMC9473185/ /pubmed/36132259 http://dx.doi.org/10.1039/c8na00331a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Jumeaux, Coline
Kim, Eunjung
Howes, Philip D.
Kim, Hyemin
Chandrawati, Rona
Stevens, Molly M.
Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion
title Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion
title_full Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion
title_fullStr Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion
title_full_unstemmed Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion
title_short Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion
title_sort detection of microrna biomarkers via inhibition of dna-mediated liposome fusion
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9473185/
https://www.ncbi.nlm.nih.gov/pubmed/36132259
http://dx.doi.org/10.1039/c8na00331a
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