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Peptide-directed synthesis of chiral nano-bipyramids for controllable antibacterial application

The emergence of antibiotic resistance makes the therapeutic effect of traditional antibiotics far from satisfactory. Here, chiral gold nano-bipyramids (GBPs) with sea cucumber-like morphology are reported, and used in the fight against bacterial infection. Specifically, the dipeptide of d-/l-Cys-Ph...

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Autores principales: Chen, Panpan, Wang, Gaoyang, Hao, Changlong, Ma, Wei, Xu, Liguang, Kuang, Hua, Xu, Chuanlai, Sun, Maozhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9473524/
https://www.ncbi.nlm.nih.gov/pubmed/36277618
http://dx.doi.org/10.1039/d2sc03443c
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author Chen, Panpan
Wang, Gaoyang
Hao, Changlong
Ma, Wei
Xu, Liguang
Kuang, Hua
Xu, Chuanlai
Sun, Maozhong
author_facet Chen, Panpan
Wang, Gaoyang
Hao, Changlong
Ma, Wei
Xu, Liguang
Kuang, Hua
Xu, Chuanlai
Sun, Maozhong
author_sort Chen, Panpan
collection PubMed
description The emergence of antibiotic resistance makes the therapeutic effect of traditional antibiotics far from satisfactory. Here, chiral gold nano-bipyramids (GBPs) with sea cucumber-like morphology are reported, and used in the fight against bacterial infection. Specifically, the dipeptide of d-/l-Cys-Phe (CF) caused the nano-bipyramids to form a spike shape with an optical anisotropy factor of 0.102 at 573 nm. The antibacterial effects showed that d-GBPs and l-GBPs could efficiently destroy bacteria with a death ratio of 98% and 70% in vitro. Also, both in vivo skin infection and sepsis models showed that the chiral GBPs could effectively promote wound healing and prevent sepsis in mice. Mechanistic studies showed that the binding affinity of d-GBPs (1.071 ± 0.023 × 10(8) M(−1)) was 12.39-fold higher than l-GBPs (8.664 ± 0.251 × 10(6) M(−1)) to protein A of Staphylococcus aureus, which caused further adsorption of d-GBPs onto the bacterial surface. Moreover, the physical destruction of the bacterial cell wall caused by the spike chiral GBPs, resulted in a stronger antibacterial effect for d-GBPs than l-GBPs. Furthermore, the excellent PTT of d-/l-GBPs further exacerbated the death of bacteria without any side-effect. Overall, chiral nano-bipyramids have opened a new avenue for improved antibacterial efficacy in the treatment of bacterial infections.
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spelling pubmed-94735242022-10-20 Peptide-directed synthesis of chiral nano-bipyramids for controllable antibacterial application Chen, Panpan Wang, Gaoyang Hao, Changlong Ma, Wei Xu, Liguang Kuang, Hua Xu, Chuanlai Sun, Maozhong Chem Sci Chemistry The emergence of antibiotic resistance makes the therapeutic effect of traditional antibiotics far from satisfactory. Here, chiral gold nano-bipyramids (GBPs) with sea cucumber-like morphology are reported, and used in the fight against bacterial infection. Specifically, the dipeptide of d-/l-Cys-Phe (CF) caused the nano-bipyramids to form a spike shape with an optical anisotropy factor of 0.102 at 573 nm. The antibacterial effects showed that d-GBPs and l-GBPs could efficiently destroy bacteria with a death ratio of 98% and 70% in vitro. Also, both in vivo skin infection and sepsis models showed that the chiral GBPs could effectively promote wound healing and prevent sepsis in mice. Mechanistic studies showed that the binding affinity of d-GBPs (1.071 ± 0.023 × 10(8) M(−1)) was 12.39-fold higher than l-GBPs (8.664 ± 0.251 × 10(6) M(−1)) to protein A of Staphylococcus aureus, which caused further adsorption of d-GBPs onto the bacterial surface. Moreover, the physical destruction of the bacterial cell wall caused by the spike chiral GBPs, resulted in a stronger antibacterial effect for d-GBPs than l-GBPs. Furthermore, the excellent PTT of d-/l-GBPs further exacerbated the death of bacteria without any side-effect. Overall, chiral nano-bipyramids have opened a new avenue for improved antibacterial efficacy in the treatment of bacterial infections. The Royal Society of Chemistry 2022-07-23 /pmc/articles/PMC9473524/ /pubmed/36277618 http://dx.doi.org/10.1039/d2sc03443c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Chen, Panpan
Wang, Gaoyang
Hao, Changlong
Ma, Wei
Xu, Liguang
Kuang, Hua
Xu, Chuanlai
Sun, Maozhong
Peptide-directed synthesis of chiral nano-bipyramids for controllable antibacterial application
title Peptide-directed synthesis of chiral nano-bipyramids for controllable antibacterial application
title_full Peptide-directed synthesis of chiral nano-bipyramids for controllable antibacterial application
title_fullStr Peptide-directed synthesis of chiral nano-bipyramids for controllable antibacterial application
title_full_unstemmed Peptide-directed synthesis of chiral nano-bipyramids for controllable antibacterial application
title_short Peptide-directed synthesis of chiral nano-bipyramids for controllable antibacterial application
title_sort peptide-directed synthesis of chiral nano-bipyramids for controllable antibacterial application
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9473524/
https://www.ncbi.nlm.nih.gov/pubmed/36277618
http://dx.doi.org/10.1039/d2sc03443c
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