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MADS-box gene AaSEP4 promotes artemisinin biosynthesis in Artemisia annua
The plant Artemisia annua is well known for its production of artemisinin, a sesquiterpene lactone that is an effective antimalarial compound. Although remarkable progress has been made toward understanding artemisinin biosynthesis, the effect of MADS-box family transcription factors on artemisinin...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9473666/ https://www.ncbi.nlm.nih.gov/pubmed/36119604 http://dx.doi.org/10.3389/fpls.2022.982317 |
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author | Chen, Tian-Tian Yao, Xing-Hao Liu, Hang Li, Yong-Peng Qin, Wei Yan, Xin Wang, Xiu-Yun Peng, Bo-Wen Zhang, Yao-Jie Shao, Jin Hu, Xin-Yi Miao, Qing Fu, Xue-Qing Wang, Yu-Liang Li, Ling Tang, Ke-Xuan |
author_facet | Chen, Tian-Tian Yao, Xing-Hao Liu, Hang Li, Yong-Peng Qin, Wei Yan, Xin Wang, Xiu-Yun Peng, Bo-Wen Zhang, Yao-Jie Shao, Jin Hu, Xin-Yi Miao, Qing Fu, Xue-Qing Wang, Yu-Liang Li, Ling Tang, Ke-Xuan |
author_sort | Chen, Tian-Tian |
collection | PubMed |
description | The plant Artemisia annua is well known for its production of artemisinin, a sesquiterpene lactone that is an effective antimalarial compound. Although remarkable progress has been made toward understanding artemisinin biosynthesis, the effect of MADS-box family transcription factors on artemisinin biosynthesis is still poorly understood. In this study, we identified a MADS transcription factor, AaSEP4, that was predominantly expressed in trichome. AaSEP4 acts as a nuclear-localized transcriptional activator activating the expression of AaGSW1 (GLANDULAR TRICHOME-SPECIFIC WRKY1). Dual-luciferase and Yeast one-hybrid assays revealed that AaSEP4 directly bound to the CArG motif in the promoter region of AaGSW1. Overexpression of AaSEP4 in A. annua significantly induced the expression of AaGSW1 and four artemisinin biosynthesis genes, including amorpha-4,11-diene synthase (ADS), cytochrome P450 monooxygenase (CYP71AV1), double-bond reductase 2 (DBR2) and aldehyde dehydrogenase 1 (ALDH1). Furthermore, the results of high-performance liquid chromatography (HPLC) showed that the artemisinin content was significantly increased in the AaSEP4-overexpressed plants. In addition, RT-qPCR results showed that AaSEP4 was induced by methyl jasmonic acid (MeJA) treatment. Taken together, these results explicitly demonstrate that AaSEP4 is a positive regulator of artemisinin biosynthesis, which can be used in the development of high-artemisinin yielding A. annua varieties. |
format | Online Article Text |
id | pubmed-9473666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94736662022-09-15 MADS-box gene AaSEP4 promotes artemisinin biosynthesis in Artemisia annua Chen, Tian-Tian Yao, Xing-Hao Liu, Hang Li, Yong-Peng Qin, Wei Yan, Xin Wang, Xiu-Yun Peng, Bo-Wen Zhang, Yao-Jie Shao, Jin Hu, Xin-Yi Miao, Qing Fu, Xue-Qing Wang, Yu-Liang Li, Ling Tang, Ke-Xuan Front Plant Sci Plant Science The plant Artemisia annua is well known for its production of artemisinin, a sesquiterpene lactone that is an effective antimalarial compound. Although remarkable progress has been made toward understanding artemisinin biosynthesis, the effect of MADS-box family transcription factors on artemisinin biosynthesis is still poorly understood. In this study, we identified a MADS transcription factor, AaSEP4, that was predominantly expressed in trichome. AaSEP4 acts as a nuclear-localized transcriptional activator activating the expression of AaGSW1 (GLANDULAR TRICHOME-SPECIFIC WRKY1). Dual-luciferase and Yeast one-hybrid assays revealed that AaSEP4 directly bound to the CArG motif in the promoter region of AaGSW1. Overexpression of AaSEP4 in A. annua significantly induced the expression of AaGSW1 and four artemisinin biosynthesis genes, including amorpha-4,11-diene synthase (ADS), cytochrome P450 monooxygenase (CYP71AV1), double-bond reductase 2 (DBR2) and aldehyde dehydrogenase 1 (ALDH1). Furthermore, the results of high-performance liquid chromatography (HPLC) showed that the artemisinin content was significantly increased in the AaSEP4-overexpressed plants. In addition, RT-qPCR results showed that AaSEP4 was induced by methyl jasmonic acid (MeJA) treatment. Taken together, these results explicitly demonstrate that AaSEP4 is a positive regulator of artemisinin biosynthesis, which can be used in the development of high-artemisinin yielding A. annua varieties. Frontiers Media S.A. 2022-08-31 /pmc/articles/PMC9473666/ /pubmed/36119604 http://dx.doi.org/10.3389/fpls.2022.982317 Text en Copyright © 2022 Chen, Yao, Liu, Li, Qin, Yan, Wang, Peng, Zhang, Shao, Hu, Miao, Fu, Wang Y-L, Li L and Tang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Chen, Tian-Tian Yao, Xing-Hao Liu, Hang Li, Yong-Peng Qin, Wei Yan, Xin Wang, Xiu-Yun Peng, Bo-Wen Zhang, Yao-Jie Shao, Jin Hu, Xin-Yi Miao, Qing Fu, Xue-Qing Wang, Yu-Liang Li, Ling Tang, Ke-Xuan MADS-box gene AaSEP4 promotes artemisinin biosynthesis in Artemisia annua |
title | MADS-box gene AaSEP4 promotes artemisinin biosynthesis in Artemisia annua |
title_full | MADS-box gene AaSEP4 promotes artemisinin biosynthesis in Artemisia annua |
title_fullStr | MADS-box gene AaSEP4 promotes artemisinin biosynthesis in Artemisia annua |
title_full_unstemmed | MADS-box gene AaSEP4 promotes artemisinin biosynthesis in Artemisia annua |
title_short | MADS-box gene AaSEP4 promotes artemisinin biosynthesis in Artemisia annua |
title_sort | mads-box gene aasep4 promotes artemisinin biosynthesis in artemisia annua |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9473666/ https://www.ncbi.nlm.nih.gov/pubmed/36119604 http://dx.doi.org/10.3389/fpls.2022.982317 |
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