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Brain atlas for glycoprotein hormone receptors at single-transcript level

There is increasing evidence that anterior pituitary hormones, traditionally thought to have unitary functions in regulating single endocrine targets, act on multiple somatic tissues, such as bone, fat, and liver. There is also emerging evidence for anterior pituitary hormone action on brain recepto...

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Autores principales: Ryu, Vitaly, Gumerova, Anisa, Korkmaz, Funda, Kang, Seong Su, Katsel, Pavel, Miyashita, Sari, Kannangara, Hasni, Cullen, Liam, Chan, Pokman, Kuo, TanChun, Padilla, Ashley, Sultana, Farhath, Wizman, Soleil A, Kramskiy, Natan, Zaidi, Samir, Kim, Se-Min, New, Maria I, Rosen, Clifford J, Goosens, Ki A, Frolinger, Tal, Haroutunian, Vahram, Ye, Keqiang, Lizneva, Daria, Davies, Terry F, Yuen, Tony, Zaidi, Mone
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9473692/
https://www.ncbi.nlm.nih.gov/pubmed/36052994
http://dx.doi.org/10.7554/eLife.79612
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author Ryu, Vitaly
Gumerova, Anisa
Korkmaz, Funda
Kang, Seong Su
Katsel, Pavel
Miyashita, Sari
Kannangara, Hasni
Cullen, Liam
Chan, Pokman
Kuo, TanChun
Padilla, Ashley
Sultana, Farhath
Wizman, Soleil A
Kramskiy, Natan
Zaidi, Samir
Kim, Se-Min
New, Maria I
Rosen, Clifford J
Goosens, Ki A
Frolinger, Tal
Haroutunian, Vahram
Ye, Keqiang
Lizneva, Daria
Davies, Terry F
Yuen, Tony
Zaidi, Mone
author_facet Ryu, Vitaly
Gumerova, Anisa
Korkmaz, Funda
Kang, Seong Su
Katsel, Pavel
Miyashita, Sari
Kannangara, Hasni
Cullen, Liam
Chan, Pokman
Kuo, TanChun
Padilla, Ashley
Sultana, Farhath
Wizman, Soleil A
Kramskiy, Natan
Zaidi, Samir
Kim, Se-Min
New, Maria I
Rosen, Clifford J
Goosens, Ki A
Frolinger, Tal
Haroutunian, Vahram
Ye, Keqiang
Lizneva, Daria
Davies, Terry F
Yuen, Tony
Zaidi, Mone
author_sort Ryu, Vitaly
collection PubMed
description There is increasing evidence that anterior pituitary hormones, traditionally thought to have unitary functions in regulating single endocrine targets, act on multiple somatic tissues, such as bone, fat, and liver. There is also emerging evidence for anterior pituitary hormone action on brain receptors in mediating central neural and peripheral somatic functions. Here, we have created the most comprehensive neuroanatomical atlas on the expression of TSHR, LHCGR, and FSHR. We have used RNAscope, a technology that allows the detection of mRNA at single-transcript level, together with protein level validation, to document Tshr expression in 173 and Fshr expression in 353 brain regions, nuclei and subnuclei identified using the Atlas for the Mouse Brain in Stereotaxic Coordinates. We also identified Lhcgr transcripts in 401 brain regions, nuclei and subnuclei. Complementarily, we used ViewRNA, another single-transcript detection technology, to establish the expression of FSHR in human brain samples, where transcripts were co-localized in MALAT1-positive neurons. In addition, we show high expression for all three receptors in the ventricular region—with yet unknown functions. Intriguingly, Tshr and Fshr expression in the ependymal layer of the third ventricle was similar to that of the thyroid follicular cells and testicular Sertoli cells, respectively. In contrast, Fshr was localized to NeuN-positive neurons in the granular layer of the dentate gyrus in murine and human brain—both are Alzheimer’s disease-vulnerable regions. Our atlas thus provides a vital resource for scientists to explore the link between the stimulation or inactivation of brain glycoprotein hormone receptors on somatic function. New actionable pathways for human disease may be unmasked through further studies.
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spelling pubmed-94736922022-09-15 Brain atlas for glycoprotein hormone receptors at single-transcript level Ryu, Vitaly Gumerova, Anisa Korkmaz, Funda Kang, Seong Su Katsel, Pavel Miyashita, Sari Kannangara, Hasni Cullen, Liam Chan, Pokman Kuo, TanChun Padilla, Ashley Sultana, Farhath Wizman, Soleil A Kramskiy, Natan Zaidi, Samir Kim, Se-Min New, Maria I Rosen, Clifford J Goosens, Ki A Frolinger, Tal Haroutunian, Vahram Ye, Keqiang Lizneva, Daria Davies, Terry F Yuen, Tony Zaidi, Mone eLife Medicine There is increasing evidence that anterior pituitary hormones, traditionally thought to have unitary functions in regulating single endocrine targets, act on multiple somatic tissues, such as bone, fat, and liver. There is also emerging evidence for anterior pituitary hormone action on brain receptors in mediating central neural and peripheral somatic functions. Here, we have created the most comprehensive neuroanatomical atlas on the expression of TSHR, LHCGR, and FSHR. We have used RNAscope, a technology that allows the detection of mRNA at single-transcript level, together with protein level validation, to document Tshr expression in 173 and Fshr expression in 353 brain regions, nuclei and subnuclei identified using the Atlas for the Mouse Brain in Stereotaxic Coordinates. We also identified Lhcgr transcripts in 401 brain regions, nuclei and subnuclei. Complementarily, we used ViewRNA, another single-transcript detection technology, to establish the expression of FSHR in human brain samples, where transcripts were co-localized in MALAT1-positive neurons. In addition, we show high expression for all three receptors in the ventricular region—with yet unknown functions. Intriguingly, Tshr and Fshr expression in the ependymal layer of the third ventricle was similar to that of the thyroid follicular cells and testicular Sertoli cells, respectively. In contrast, Fshr was localized to NeuN-positive neurons in the granular layer of the dentate gyrus in murine and human brain—both are Alzheimer’s disease-vulnerable regions. Our atlas thus provides a vital resource for scientists to explore the link between the stimulation or inactivation of brain glycoprotein hormone receptors on somatic function. New actionable pathways for human disease may be unmasked through further studies. eLife Sciences Publications, Ltd 2022-09-02 /pmc/articles/PMC9473692/ /pubmed/36052994 http://dx.doi.org/10.7554/eLife.79612 Text en © 2022, Ryu et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Medicine
Ryu, Vitaly
Gumerova, Anisa
Korkmaz, Funda
Kang, Seong Su
Katsel, Pavel
Miyashita, Sari
Kannangara, Hasni
Cullen, Liam
Chan, Pokman
Kuo, TanChun
Padilla, Ashley
Sultana, Farhath
Wizman, Soleil A
Kramskiy, Natan
Zaidi, Samir
Kim, Se-Min
New, Maria I
Rosen, Clifford J
Goosens, Ki A
Frolinger, Tal
Haroutunian, Vahram
Ye, Keqiang
Lizneva, Daria
Davies, Terry F
Yuen, Tony
Zaidi, Mone
Brain atlas for glycoprotein hormone receptors at single-transcript level
title Brain atlas for glycoprotein hormone receptors at single-transcript level
title_full Brain atlas for glycoprotein hormone receptors at single-transcript level
title_fullStr Brain atlas for glycoprotein hormone receptors at single-transcript level
title_full_unstemmed Brain atlas for glycoprotein hormone receptors at single-transcript level
title_short Brain atlas for glycoprotein hormone receptors at single-transcript level
title_sort brain atlas for glycoprotein hormone receptors at single-transcript level
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9473692/
https://www.ncbi.nlm.nih.gov/pubmed/36052994
http://dx.doi.org/10.7554/eLife.79612
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