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Integrating an aerosolized drug delivery device with conventional static cultures and a dynamic airway barrier microphysiological system

Organ on a chip or microphysiological systems (MPSs) aim to resolve current challenges surrounding drug discovery and development resulting from an unrepresentative static cell culture or animal models that are traditionally used by generating a more physiologically relevant environment. Many differ...

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Detalles Bibliográficos
Autores principales: Karra, Nikita, Fernandes, Joao, Swindle, Emily Jane, Morgan, Hywel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9473724/
https://www.ncbi.nlm.nih.gov/pubmed/36118260
http://dx.doi.org/10.1063/5.0100019
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author Karra, Nikita
Fernandes, Joao
Swindle, Emily Jane
Morgan, Hywel
author_facet Karra, Nikita
Fernandes, Joao
Swindle, Emily Jane
Morgan, Hywel
author_sort Karra, Nikita
collection PubMed
description Organ on a chip or microphysiological systems (MPSs) aim to resolve current challenges surrounding drug discovery and development resulting from an unrepresentative static cell culture or animal models that are traditionally used by generating a more physiologically relevant environment. Many different airway MPSs have been developed that mimic alveolar or bronchial interfaces, but few methods for aerosol drug delivery at the air–liquid interface exist. This work demonstrates a compact Surface Acoustic Wave (SAW) drug delivery device that generates an aerosol of respirable size for delivery of compounds directly onto polarized or differentiated epithelial cell cultures within an airway barrier MPS and conventional static inserts. As proof of principle, the SAW drug delivery device was used to nebulize viral dsRNA analog poly I:C and steroids fluticasone and dexamethasone without disrupting their biological function.
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spelling pubmed-94737242022-09-15 Integrating an aerosolized drug delivery device with conventional static cultures and a dynamic airway barrier microphysiological system Karra, Nikita Fernandes, Joao Swindle, Emily Jane Morgan, Hywel Biomicrofluidics Regular Articles Organ on a chip or microphysiological systems (MPSs) aim to resolve current challenges surrounding drug discovery and development resulting from an unrepresentative static cell culture or animal models that are traditionally used by generating a more physiologically relevant environment. Many different airway MPSs have been developed that mimic alveolar or bronchial interfaces, but few methods for aerosol drug delivery at the air–liquid interface exist. This work demonstrates a compact Surface Acoustic Wave (SAW) drug delivery device that generates an aerosol of respirable size for delivery of compounds directly onto polarized or differentiated epithelial cell cultures within an airway barrier MPS and conventional static inserts. As proof of principle, the SAW drug delivery device was used to nebulize viral dsRNA analog poly I:C and steroids fluticasone and dexamethasone without disrupting their biological function. AIP Publishing LLC 2022-09-13 /pmc/articles/PMC9473724/ /pubmed/36118260 http://dx.doi.org/10.1063/5.0100019 Text en © 2022 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). Published open access through an agreement withJISC Collections128554
spellingShingle Regular Articles
Karra, Nikita
Fernandes, Joao
Swindle, Emily Jane
Morgan, Hywel
Integrating an aerosolized drug delivery device with conventional static cultures and a dynamic airway barrier microphysiological system
title Integrating an aerosolized drug delivery device with conventional static cultures and a dynamic airway barrier microphysiological system
title_full Integrating an aerosolized drug delivery device with conventional static cultures and a dynamic airway barrier microphysiological system
title_fullStr Integrating an aerosolized drug delivery device with conventional static cultures and a dynamic airway barrier microphysiological system
title_full_unstemmed Integrating an aerosolized drug delivery device with conventional static cultures and a dynamic airway barrier microphysiological system
title_short Integrating an aerosolized drug delivery device with conventional static cultures and a dynamic airway barrier microphysiological system
title_sort integrating an aerosolized drug delivery device with conventional static cultures and a dynamic airway barrier microphysiological system
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9473724/
https://www.ncbi.nlm.nih.gov/pubmed/36118260
http://dx.doi.org/10.1063/5.0100019
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