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Method for identification of 10 SSR markers from monkey genomes and its statistical inference with One & Two-way ANOVA

DNA tracts that include simple sequence repeats (SSRs), sometimes known as genetic "stutters), are composed of a few to many tandem repetitions of a short base-pair motif. These sequences frequently mutate, changing the amount of repetitions. SSRs are frequently found in promoters, untranslated...

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Autores principales: Rao, Chinta Someswara, Sirisha, G.N.V.G., Raju, K. Butchi, Raju, N V Ganapathi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9474309/
https://www.ncbi.nlm.nih.gov/pubmed/36117677
http://dx.doi.org/10.1016/j.mex.2022.101833
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author Rao, Chinta Someswara
Sirisha, G.N.V.G.
Raju, K. Butchi
Raju, N V Ganapathi
author_facet Rao, Chinta Someswara
Sirisha, G.N.V.G.
Raju, K. Butchi
Raju, N V Ganapathi
author_sort Rao, Chinta Someswara
collection PubMed
description DNA tracts that include simple sequence repeats (SSRs), sometimes known as genetic "stutters), are composed of a few to many tandem repetitions of a short base-pair motif. These sequences frequently mutate, changing the amount of repetitions. SSRs are frequently found in promoters, untranslated regions, and even coding sequences, therefore these alterations can significantly affect practically every aspect of gene activity. SSR alleles can also contribute to normal diversity in brain and behavioural features. Mutational expansion of certain triplet repeats is the cause of a number of inherited neurodegenerative diseases. Due to its importance in genetic research, in this paper we explored Ten SSR markers TAGA, TCAT, GAAT, AGAT, AGAA, GATA, TATC, CTTT, TCTG and TCTA that are identified from the genomes of Eleven distinct monkeys: A.Nancymaae, C.C.Imitator, C.Atys, M.Leucophaeus, P.Paniscus, R.Bieti, R.Roxellana, S.Boliviensis, T.Syrichta, C.A.Palliatus and M.Nemestrina using pattern matching mechanism. We identified 4bp SSR from eleven monkey dataset's Unchr chromosome mainly in this paper. The proposed approach finds the exact place/location of the SSR's and number of times that it appears in the given genome sequence. The identified patterns are analyzed with One-way and Two-way ANOVA that gives better analysis which is useful for genomic studies. Also, this 4bp Ten SSR markers data is a valuable to illustrate genetic variation of genomic study. • The great specificity of data sets produced from monkey genomes with pattern matching has been demonstrated. • These findings show that SSR identification could be a useful tool for determining genome similarity and comparability. • Researchers can use the raw sequencing data to conduct additional bioinformatics analysis.
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spelling pubmed-94743092022-09-16 Method for identification of 10 SSR markers from monkey genomes and its statistical inference with One & Two-way ANOVA Rao, Chinta Someswara Sirisha, G.N.V.G. Raju, K. Butchi Raju, N V Ganapathi MethodsX Method Article DNA tracts that include simple sequence repeats (SSRs), sometimes known as genetic "stutters), are composed of a few to many tandem repetitions of a short base-pair motif. These sequences frequently mutate, changing the amount of repetitions. SSRs are frequently found in promoters, untranslated regions, and even coding sequences, therefore these alterations can significantly affect practically every aspect of gene activity. SSR alleles can also contribute to normal diversity in brain and behavioural features. Mutational expansion of certain triplet repeats is the cause of a number of inherited neurodegenerative diseases. Due to its importance in genetic research, in this paper we explored Ten SSR markers TAGA, TCAT, GAAT, AGAT, AGAA, GATA, TATC, CTTT, TCTG and TCTA that are identified from the genomes of Eleven distinct monkeys: A.Nancymaae, C.C.Imitator, C.Atys, M.Leucophaeus, P.Paniscus, R.Bieti, R.Roxellana, S.Boliviensis, T.Syrichta, C.A.Palliatus and M.Nemestrina using pattern matching mechanism. We identified 4bp SSR from eleven monkey dataset's Unchr chromosome mainly in this paper. The proposed approach finds the exact place/location of the SSR's and number of times that it appears in the given genome sequence. The identified patterns are analyzed with One-way and Two-way ANOVA that gives better analysis which is useful for genomic studies. Also, this 4bp Ten SSR markers data is a valuable to illustrate genetic variation of genomic study. • The great specificity of data sets produced from monkey genomes with pattern matching has been demonstrated. • These findings show that SSR identification could be a useful tool for determining genome similarity and comparability. • Researchers can use the raw sequencing data to conduct additional bioinformatics analysis. Elsevier 2022-08-30 /pmc/articles/PMC9474309/ /pubmed/36117677 http://dx.doi.org/10.1016/j.mex.2022.101833 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Method Article
Rao, Chinta Someswara
Sirisha, G.N.V.G.
Raju, K. Butchi
Raju, N V Ganapathi
Method for identification of 10 SSR markers from monkey genomes and its statistical inference with One & Two-way ANOVA
title Method for identification of 10 SSR markers from monkey genomes and its statistical inference with One & Two-way ANOVA
title_full Method for identification of 10 SSR markers from monkey genomes and its statistical inference with One & Two-way ANOVA
title_fullStr Method for identification of 10 SSR markers from monkey genomes and its statistical inference with One & Two-way ANOVA
title_full_unstemmed Method for identification of 10 SSR markers from monkey genomes and its statistical inference with One & Two-way ANOVA
title_short Method for identification of 10 SSR markers from monkey genomes and its statistical inference with One & Two-way ANOVA
title_sort method for identification of 10 ssr markers from monkey genomes and its statistical inference with one & two-way anova
topic Method Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9474309/
https://www.ncbi.nlm.nih.gov/pubmed/36117677
http://dx.doi.org/10.1016/j.mex.2022.101833
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