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Layered feedback control overcomes performance trade-off in synthetic biomolecular networks

Layered feedback is an optimization strategy in feedback control designs widely used in engineering. Control theory suggests that layering multiple feedbacks could overcome the robustness-speed performance trade-off limit. In natural biological networks, genes are often regulated in layers to adapt...

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Autores principales: Hu, Chelsea Y., Murray, Richard M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9474519/
https://www.ncbi.nlm.nih.gov/pubmed/36104365
http://dx.doi.org/10.1038/s41467-022-33058-6
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author Hu, Chelsea Y.
Murray, Richard M.
author_facet Hu, Chelsea Y.
Murray, Richard M.
author_sort Hu, Chelsea Y.
collection PubMed
description Layered feedback is an optimization strategy in feedback control designs widely used in engineering. Control theory suggests that layering multiple feedbacks could overcome the robustness-speed performance trade-off limit. In natural biological networks, genes are often regulated in layers to adapt to environmental perturbations. It is hypothesized layering architecture could also overcome the robustness-speed performance trade-off in genetic networks. In this work, we validate this hypothesis with a synthetic biomolecular network in living E. coli cells. We start with system dynamics analysis using models of various complexities to guide the design of a layered control architecture in living cells. Experimentally, we interrogate system dynamics under three groups of perturbations. We consistently observe that the layered control improves system performance in the robustness-speed domain. This work confirms that layered control could be adopted in synthetic biomolecular networks for performance optimization. It also provides insights into understanding genetic feedback control architectures in nature.
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spelling pubmed-94745192022-09-16 Layered feedback control overcomes performance trade-off in synthetic biomolecular networks Hu, Chelsea Y. Murray, Richard M. Nat Commun Article Layered feedback is an optimization strategy in feedback control designs widely used in engineering. Control theory suggests that layering multiple feedbacks could overcome the robustness-speed performance trade-off limit. In natural biological networks, genes are often regulated in layers to adapt to environmental perturbations. It is hypothesized layering architecture could also overcome the robustness-speed performance trade-off in genetic networks. In this work, we validate this hypothesis with a synthetic biomolecular network in living E. coli cells. We start with system dynamics analysis using models of various complexities to guide the design of a layered control architecture in living cells. Experimentally, we interrogate system dynamics under three groups of perturbations. We consistently observe that the layered control improves system performance in the robustness-speed domain. This work confirms that layered control could be adopted in synthetic biomolecular networks for performance optimization. It also provides insights into understanding genetic feedback control architectures in nature. Nature Publishing Group UK 2022-09-14 /pmc/articles/PMC9474519/ /pubmed/36104365 http://dx.doi.org/10.1038/s41467-022-33058-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hu, Chelsea Y.
Murray, Richard M.
Layered feedback control overcomes performance trade-off in synthetic biomolecular networks
title Layered feedback control overcomes performance trade-off in synthetic biomolecular networks
title_full Layered feedback control overcomes performance trade-off in synthetic biomolecular networks
title_fullStr Layered feedback control overcomes performance trade-off in synthetic biomolecular networks
title_full_unstemmed Layered feedback control overcomes performance trade-off in synthetic biomolecular networks
title_short Layered feedback control overcomes performance trade-off in synthetic biomolecular networks
title_sort layered feedback control overcomes performance trade-off in synthetic biomolecular networks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9474519/
https://www.ncbi.nlm.nih.gov/pubmed/36104365
http://dx.doi.org/10.1038/s41467-022-33058-6
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