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The p-ERG spatial acuity in the biomedical pig under physiological conditions

Pigs are becoming an important pre-clinical animal species for translational ophthalmology, due to similarities with humans in anatomical and physiological patterns. Different models of eye disorders have been proposed, and they are good candidates to assess biocompatibility/functionality of retinal...

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Autores principales: Ventrella, Domenico, Maya-Vetencourt, José Fernando, Elmi, Alberto, Barone, Francesca, Aniballi, Camilla, Muscatello, Luisa Vera, Mete, Maurizio, Pertile, Grazia, Benfenati, Fabio, Bacci, Maria Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9474814/
https://www.ncbi.nlm.nih.gov/pubmed/36104429
http://dx.doi.org/10.1038/s41598-022-19925-8
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author Ventrella, Domenico
Maya-Vetencourt, José Fernando
Elmi, Alberto
Barone, Francesca
Aniballi, Camilla
Muscatello, Luisa Vera
Mete, Maurizio
Pertile, Grazia
Benfenati, Fabio
Bacci, Maria Laura
author_facet Ventrella, Domenico
Maya-Vetencourt, José Fernando
Elmi, Alberto
Barone, Francesca
Aniballi, Camilla
Muscatello, Luisa Vera
Mete, Maurizio
Pertile, Grazia
Benfenati, Fabio
Bacci, Maria Laura
author_sort Ventrella, Domenico
collection PubMed
description Pigs are becoming an important pre-clinical animal species for translational ophthalmology, due to similarities with humans in anatomical and physiological patterns. Different models of eye disorders have been proposed, and they are good candidates to assess biocompatibility/functionality of retinal prostheses. Electroretinography is a common tool allowing to gain information on retinal function, with several types of electroretinogram (ERG) been implemented including full field (ff-ERG), multifocal (mf-ERG) and pattern (p-ERG). p-ERG represents a valuable tool to monitor Retinal Ganglion Cells (RGCs) activity and can be used to calculate p-ERG spatial acuity. Unfortunately, scarce methodological data are available regarding recording/interpretation of p-ERG and retinal acuity in biomedical pigs yet enhancing knowledge regarding pig vision physiology will allow for more refined and responsible use of such species. Aim of this study was to record p-ERG in juvenile pigs to functionally assess visual acuity. Six female hybrid pigs underwent two p-ERG recording sessions at 16 and 19 weeks of age. Photopic ff-ERG were also recorded; optical coherence tomography (OCT) and histology were used to confirm retinal integrity. ff-ERG signals were repeatable within/across sessions. All p-ERG traces consistently displayed characterizing peaks, and the progressive decrease of amplitude in response to the increment of spatial frequency revealed the reliability of the method. Mean p-ERG spatial acuities were 5.7 ± 0.14 (16 weeks) and 6.2 ± 0.15 cpd (19 weeks). Overall, the p-ERG recordings described in the present work seem reliable and repeatable, and may represent an important tool when it comes to vision assessment in pigs.
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spelling pubmed-94748142022-09-16 The p-ERG spatial acuity in the biomedical pig under physiological conditions Ventrella, Domenico Maya-Vetencourt, José Fernando Elmi, Alberto Barone, Francesca Aniballi, Camilla Muscatello, Luisa Vera Mete, Maurizio Pertile, Grazia Benfenati, Fabio Bacci, Maria Laura Sci Rep Article Pigs are becoming an important pre-clinical animal species for translational ophthalmology, due to similarities with humans in anatomical and physiological patterns. Different models of eye disorders have been proposed, and they are good candidates to assess biocompatibility/functionality of retinal prostheses. Electroretinography is a common tool allowing to gain information on retinal function, with several types of electroretinogram (ERG) been implemented including full field (ff-ERG), multifocal (mf-ERG) and pattern (p-ERG). p-ERG represents a valuable tool to monitor Retinal Ganglion Cells (RGCs) activity and can be used to calculate p-ERG spatial acuity. Unfortunately, scarce methodological data are available regarding recording/interpretation of p-ERG and retinal acuity in biomedical pigs yet enhancing knowledge regarding pig vision physiology will allow for more refined and responsible use of such species. Aim of this study was to record p-ERG in juvenile pigs to functionally assess visual acuity. Six female hybrid pigs underwent two p-ERG recording sessions at 16 and 19 weeks of age. Photopic ff-ERG were also recorded; optical coherence tomography (OCT) and histology were used to confirm retinal integrity. ff-ERG signals were repeatable within/across sessions. All p-ERG traces consistently displayed characterizing peaks, and the progressive decrease of amplitude in response to the increment of spatial frequency revealed the reliability of the method. Mean p-ERG spatial acuities were 5.7 ± 0.14 (16 weeks) and 6.2 ± 0.15 cpd (19 weeks). Overall, the p-ERG recordings described in the present work seem reliable and repeatable, and may represent an important tool when it comes to vision assessment in pigs. Nature Publishing Group UK 2022-09-14 /pmc/articles/PMC9474814/ /pubmed/36104429 http://dx.doi.org/10.1038/s41598-022-19925-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ventrella, Domenico
Maya-Vetencourt, José Fernando
Elmi, Alberto
Barone, Francesca
Aniballi, Camilla
Muscatello, Luisa Vera
Mete, Maurizio
Pertile, Grazia
Benfenati, Fabio
Bacci, Maria Laura
The p-ERG spatial acuity in the biomedical pig under physiological conditions
title The p-ERG spatial acuity in the biomedical pig under physiological conditions
title_full The p-ERG spatial acuity in the biomedical pig under physiological conditions
title_fullStr The p-ERG spatial acuity in the biomedical pig under physiological conditions
title_full_unstemmed The p-ERG spatial acuity in the biomedical pig under physiological conditions
title_short The p-ERG spatial acuity in the biomedical pig under physiological conditions
title_sort p-erg spatial acuity in the biomedical pig under physiological conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9474814/
https://www.ncbi.nlm.nih.gov/pubmed/36104429
http://dx.doi.org/10.1038/s41598-022-19925-8
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