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DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect

[Image: see text] A long-term reusable sensor that provides the opportunity to easily regenerate the active surface and minimize the occurrence of undesired absorption events is an appealing solution that helps to cut down the costs and improve the device performances. Impressive advances have been...

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Autores principales: Trojanowicz, Remigiusz K., Vestri, Ambra, Rippa, Massimo, Zyss, Joseph, Matczyszyn, Katarzyna, Petti, Lucia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9475616/
https://www.ncbi.nlm.nih.gov/pubmed/36120011
http://dx.doi.org/10.1021/acsomega.2c01370
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author Trojanowicz, Remigiusz K.
Vestri, Ambra
Rippa, Massimo
Zyss, Joseph
Matczyszyn, Katarzyna
Petti, Lucia
author_facet Trojanowicz, Remigiusz K.
Vestri, Ambra
Rippa, Massimo
Zyss, Joseph
Matczyszyn, Katarzyna
Petti, Lucia
author_sort Trojanowicz, Remigiusz K.
collection PubMed
description [Image: see text] A long-term reusable sensor that provides the opportunity to easily regenerate the active surface and minimize the occurrence of undesired absorption events is an appealing solution that helps to cut down the costs and improve the device performances. Impressive advances have been made in the past years concerning the development of novel cutting-edge sensors, but the reusability can currently represent a challenge. Direct shielding of the sensor surface is not always applicable, because it can impact the device performance. This study reports an antiadhesive layer (AAL) made of 90 mg/mL DNA sodium salt from salmon testes (ssstDNA) for passivating gold plasmonic sensor surfaces. Our gold two-dimensional (2D) nanostructured plasmonic metasurfaces modified with AAL were used for DNA quantification. AAL is thin enough that the plasmonic sensor remains sensitive to subsequent deposition of DNA, which serves as an analyte. AAL protects the gold surface from unwanted nonspecific adsorption by enabling wash-off of the deposited analyte after analysis and thus recovery of the LSPR peak position (rLSPR). The calibration curve obtained on a single nanostructure (Achiral Octupolar, 100 nm pitch) gave an LOD = 105 ng/mL and an extraordinary dynamic range, performances comparable or superior to those of commercial UV–vis spectrometers for acid nucleic dosage. Two different analytes were tested: ssstDNA (∼2000 bp) in deionized water and double-strand DNA (dsDNA) of 546–1614 bp in 100 mM Tris buffer and 10 mM MgCl(2). The two nanostructures (Achiral Octupolar 25 and 100) were found to have the same sensitivity to DNA in deionized water but different sensitivity to DNA in a salt/buffer solution, opening a potential for solute discrimination. To the best of our knowledge, this is the first report on the use of AAL made of several kilobase-pairs-long dsDNA to produce a reusable plasmonic sensor. The working principle and limitations are drawn based on the LSPR and SERS study.
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spelling pubmed-94756162022-09-16 DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect Trojanowicz, Remigiusz K. Vestri, Ambra Rippa, Massimo Zyss, Joseph Matczyszyn, Katarzyna Petti, Lucia ACS Omega [Image: see text] A long-term reusable sensor that provides the opportunity to easily regenerate the active surface and minimize the occurrence of undesired absorption events is an appealing solution that helps to cut down the costs and improve the device performances. Impressive advances have been made in the past years concerning the development of novel cutting-edge sensors, but the reusability can currently represent a challenge. Direct shielding of the sensor surface is not always applicable, because it can impact the device performance. This study reports an antiadhesive layer (AAL) made of 90 mg/mL DNA sodium salt from salmon testes (ssstDNA) for passivating gold plasmonic sensor surfaces. Our gold two-dimensional (2D) nanostructured plasmonic metasurfaces modified with AAL were used for DNA quantification. AAL is thin enough that the plasmonic sensor remains sensitive to subsequent deposition of DNA, which serves as an analyte. AAL protects the gold surface from unwanted nonspecific adsorption by enabling wash-off of the deposited analyte after analysis and thus recovery of the LSPR peak position (rLSPR). The calibration curve obtained on a single nanostructure (Achiral Octupolar, 100 nm pitch) gave an LOD = 105 ng/mL and an extraordinary dynamic range, performances comparable or superior to those of commercial UV–vis spectrometers for acid nucleic dosage. Two different analytes were tested: ssstDNA (∼2000 bp) in deionized water and double-strand DNA (dsDNA) of 546–1614 bp in 100 mM Tris buffer and 10 mM MgCl(2). The two nanostructures (Achiral Octupolar 25 and 100) were found to have the same sensitivity to DNA in deionized water but different sensitivity to DNA in a salt/buffer solution, opening a potential for solute discrimination. To the best of our knowledge, this is the first report on the use of AAL made of several kilobase-pairs-long dsDNA to produce a reusable plasmonic sensor. The working principle and limitations are drawn based on the LSPR and SERS study. American Chemical Society 2022-08-30 /pmc/articles/PMC9475616/ /pubmed/36120011 http://dx.doi.org/10.1021/acsomega.2c01370 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Trojanowicz, Remigiusz K.
Vestri, Ambra
Rippa, Massimo
Zyss, Joseph
Matczyszyn, Katarzyna
Petti, Lucia
DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect
title DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect
title_full DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect
title_fullStr DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect
title_full_unstemmed DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect
title_short DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect
title_sort dna antiadhesive layer for reusable plasmonic sensors: nanostructure pitch effect
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9475616/
https://www.ncbi.nlm.nih.gov/pubmed/36120011
http://dx.doi.org/10.1021/acsomega.2c01370
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