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Mitochondrial ATP production is impaired in neural stem/progenitor cells derived from olfactory neuroepithelium of patients with schizophrenia

INTRODUCTION: Neural stem/progenitor cells derived from olfactory neuroepithelium (hereafter OE-NS/PCs) are emerging as a viable proxy and a valuable tool for translational studies on severe mental illnesses (SMI). In this respect, the use of OE-NS/PCs as a surrogate cellular model of schizophrenia...

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Autores principales: Idotta, C., Tibaldi, E., Favaretto, N., Pagano, M., Peruzzo, R., Pigato, G., Cazzador, D., Meneguzzo, P., Solmi, M., Leanza, L., Favaro, A., Brunati, A.M., Toffanin, T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9475769/
http://dx.doi.org/10.1192/j.eurpsy.2021.1026
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author Idotta, C.
Tibaldi, E.
Favaretto, N.
Pagano, M.
Peruzzo, R.
Pigato, G.
Cazzador, D.
Meneguzzo, P.
Solmi, M.
Leanza, L.
Favaro, A.
Brunati, A.M.
Toffanin, T.
author_facet Idotta, C.
Tibaldi, E.
Favaretto, N.
Pagano, M.
Peruzzo, R.
Pigato, G.
Cazzador, D.
Meneguzzo, P.
Solmi, M.
Leanza, L.
Favaro, A.
Brunati, A.M.
Toffanin, T.
author_sort Idotta, C.
collection PubMed
description INTRODUCTION: Neural stem/progenitor cells derived from olfactory neuroepithelium (hereafter OE-NS/PCs) are emerging as a viable proxy and a valuable tool for translational studies on severe mental illnesses (SMI). In this respect, the use of OE-NS/PCs as a surrogate cellular model of schizophrenia (SZ) has enabled insights into cell signaling and cell cycle dynamics in this disease. OBJECTIVES: We explored whether mitochondrial dysfunction, which has been already associated with SZ, may have a role in the altered proliferation pattern previously observed in OE-NS/PCs of SZ patients. METHODS: OE-NS/PCs were collected from 20 patients and 20 healthy controls (Hcs) by nasal brushing, cultured in proper medium and expanded. Fresh OE-NS/PCs at passage 3 of both groups underwent BrdU proliferation assays or were frozen for later use. Mitochondrial ATP production was measured in both fresh and thawed OE-NS/PCs by using the ATPlite Luminescence Assay kit. RESULTS: Fresh OE-NS/PCs of patients grew at a higher rate than those of HCs (M-W U=0; p<0.001), whereas the proliferation of thawed OE-NS/PCs of both groups exhibited an opposed pattern (at passage 6, p=0.002). Mitochondrial ATP production was significantly lower in OE-NS/PCs of patients than in those of HCs (M-W U=0; p=0.02), regardless of freeze-thaw conditions (M-W U=6; p=0.77). CONCLUSIONS: Mitochondrial ATP production is negatively affected in OE-NS/PCs of SZ patients as compared to those of HCs. This evidence does not differ in fresh OE-NS/PCs and OE-NS/PCs undergoing freeze-thaw cycles, which instead perturb the proliferation pattern of SZ OE-NS/PCs.
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spelling pubmed-94757692022-09-29 Mitochondrial ATP production is impaired in neural stem/progenitor cells derived from olfactory neuroepithelium of patients with schizophrenia Idotta, C. Tibaldi, E. Favaretto, N. Pagano, M. Peruzzo, R. Pigato, G. Cazzador, D. Meneguzzo, P. Solmi, M. Leanza, L. Favaro, A. Brunati, A.M. Toffanin, T. Eur Psychiatry Abstract INTRODUCTION: Neural stem/progenitor cells derived from olfactory neuroepithelium (hereafter OE-NS/PCs) are emerging as a viable proxy and a valuable tool for translational studies on severe mental illnesses (SMI). In this respect, the use of OE-NS/PCs as a surrogate cellular model of schizophrenia (SZ) has enabled insights into cell signaling and cell cycle dynamics in this disease. OBJECTIVES: We explored whether mitochondrial dysfunction, which has been already associated with SZ, may have a role in the altered proliferation pattern previously observed in OE-NS/PCs of SZ patients. METHODS: OE-NS/PCs were collected from 20 patients and 20 healthy controls (Hcs) by nasal brushing, cultured in proper medium and expanded. Fresh OE-NS/PCs at passage 3 of both groups underwent BrdU proliferation assays or were frozen for later use. Mitochondrial ATP production was measured in both fresh and thawed OE-NS/PCs by using the ATPlite Luminescence Assay kit. RESULTS: Fresh OE-NS/PCs of patients grew at a higher rate than those of HCs (M-W U=0; p<0.001), whereas the proliferation of thawed OE-NS/PCs of both groups exhibited an opposed pattern (at passage 6, p=0.002). Mitochondrial ATP production was significantly lower in OE-NS/PCs of patients than in those of HCs (M-W U=0; p=0.02), regardless of freeze-thaw conditions (M-W U=6; p=0.77). CONCLUSIONS: Mitochondrial ATP production is negatively affected in OE-NS/PCs of SZ patients as compared to those of HCs. This evidence does not differ in fresh OE-NS/PCs and OE-NS/PCs undergoing freeze-thaw cycles, which instead perturb the proliferation pattern of SZ OE-NS/PCs. Cambridge University Press 2021-08-13 /pmc/articles/PMC9475769/ http://dx.doi.org/10.1192/j.eurpsy.2021.1026 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Abstract
Idotta, C.
Tibaldi, E.
Favaretto, N.
Pagano, M.
Peruzzo, R.
Pigato, G.
Cazzador, D.
Meneguzzo, P.
Solmi, M.
Leanza, L.
Favaro, A.
Brunati, A.M.
Toffanin, T.
Mitochondrial ATP production is impaired in neural stem/progenitor cells derived from olfactory neuroepithelium of patients with schizophrenia
title Mitochondrial ATP production is impaired in neural stem/progenitor cells derived from olfactory neuroepithelium of patients with schizophrenia
title_full Mitochondrial ATP production is impaired in neural stem/progenitor cells derived from olfactory neuroepithelium of patients with schizophrenia
title_fullStr Mitochondrial ATP production is impaired in neural stem/progenitor cells derived from olfactory neuroepithelium of patients with schizophrenia
title_full_unstemmed Mitochondrial ATP production is impaired in neural stem/progenitor cells derived from olfactory neuroepithelium of patients with schizophrenia
title_short Mitochondrial ATP production is impaired in neural stem/progenitor cells derived from olfactory neuroepithelium of patients with schizophrenia
title_sort mitochondrial atp production is impaired in neural stem/progenitor cells derived from olfactory neuroepithelium of patients with schizophrenia
topic Abstract
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9475769/
http://dx.doi.org/10.1192/j.eurpsy.2021.1026
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