In Vivo Detection of Cyclic-di-AMP in Staphylococcus aureus

[Image: see text] Cyclic-di-AMP (CDA) is a signaling molecule that controls various cellular functions including antibiotic tolerance and osmoregulation in Staphylococcus aureus (S. aureus). In this study, we developed a novel biosensor (bsuO P6-4) for in vivo detection of CDA in S. aureus. The fluo...

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Autores principales: Mukkayyan, Nagaraja, Poon, Raymond, Sander, Philipp N., Lai, Li-Yin, Zubair-Nizami, Zahra, Hammond, Ming C., Chatterjee, Som S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476191/
https://www.ncbi.nlm.nih.gov/pubmed/36120079
http://dx.doi.org/10.1021/acsomega.2c04538
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author Mukkayyan, Nagaraja
Poon, Raymond
Sander, Philipp N.
Lai, Li-Yin
Zubair-Nizami, Zahra
Hammond, Ming C.
Chatterjee, Som S.
author_facet Mukkayyan, Nagaraja
Poon, Raymond
Sander, Philipp N.
Lai, Li-Yin
Zubair-Nizami, Zahra
Hammond, Ming C.
Chatterjee, Som S.
author_sort Mukkayyan, Nagaraja
collection PubMed
description [Image: see text] Cyclic-di-AMP (CDA) is a signaling molecule that controls various cellular functions including antibiotic tolerance and osmoregulation in Staphylococcus aureus (S. aureus). In this study, we developed a novel biosensor (bsuO P6-4) for in vivo detection of CDA in S. aureus. The fluorescent biosensor is based on a natural CDA riboswitch from Bacillus subtilis connected at its P6 stem to the dye-binding aptamer Spinach. Our study showed that bsuO P6-4 could detect a wide concentration range of CDA in both laboratory and clinical strains, making it suitable for use in both basic and clinical research applications.
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spelling pubmed-94761912022-09-16 In Vivo Detection of Cyclic-di-AMP in Staphylococcus aureus Mukkayyan, Nagaraja Poon, Raymond Sander, Philipp N. Lai, Li-Yin Zubair-Nizami, Zahra Hammond, Ming C. Chatterjee, Som S. ACS Omega [Image: see text] Cyclic-di-AMP (CDA) is a signaling molecule that controls various cellular functions including antibiotic tolerance and osmoregulation in Staphylococcus aureus (S. aureus). In this study, we developed a novel biosensor (bsuO P6-4) for in vivo detection of CDA in S. aureus. The fluorescent biosensor is based on a natural CDA riboswitch from Bacillus subtilis connected at its P6 stem to the dye-binding aptamer Spinach. Our study showed that bsuO P6-4 could detect a wide concentration range of CDA in both laboratory and clinical strains, making it suitable for use in both basic and clinical research applications. American Chemical Society 2022-08-26 /pmc/articles/PMC9476191/ /pubmed/36120079 http://dx.doi.org/10.1021/acsomega.2c04538 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Mukkayyan, Nagaraja
Poon, Raymond
Sander, Philipp N.
Lai, Li-Yin
Zubair-Nizami, Zahra
Hammond, Ming C.
Chatterjee, Som S.
In Vivo Detection of Cyclic-di-AMP in Staphylococcus aureus
title In Vivo Detection of Cyclic-di-AMP in Staphylococcus aureus
title_full In Vivo Detection of Cyclic-di-AMP in Staphylococcus aureus
title_fullStr In Vivo Detection of Cyclic-di-AMP in Staphylococcus aureus
title_full_unstemmed In Vivo Detection of Cyclic-di-AMP in Staphylococcus aureus
title_short In Vivo Detection of Cyclic-di-AMP in Staphylococcus aureus
title_sort in vivo detection of cyclic-di-amp in staphylococcus aureus
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476191/
https://www.ncbi.nlm.nih.gov/pubmed/36120079
http://dx.doi.org/10.1021/acsomega.2c04538
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