Cargando…

Surface-Coated Cerium Nanoparticles to Improve Chemotherapeutic Delivery to Tumor Cells

[Image: see text] The antioxidant property of cerium oxide nanoparticles has increased their demand as a nanocarrier to improve the delivery and therapeutic efficacy of anticancer drugs. Here, we report the synthesis of alginate-coated ceria nanoformulations (ceria NPs) and characterization using FT...

Descripción completa

Detalles Bibliográficos
Autores principales: Pramanik, Nilkamal, De, Tamasa, Sharma, Preeti, Alakesh, Alakesh, Jagirdar, Sameer Kumar, Rangarajan, Annapoorni, Jhunjhunwala, Siddharth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476200/
https://www.ncbi.nlm.nih.gov/pubmed/36120021
http://dx.doi.org/10.1021/acsomega.2c00062
_version_ 1784790087157940224
author Pramanik, Nilkamal
De, Tamasa
Sharma, Preeti
Alakesh, Alakesh
Jagirdar, Sameer Kumar
Rangarajan, Annapoorni
Jhunjhunwala, Siddharth
author_facet Pramanik, Nilkamal
De, Tamasa
Sharma, Preeti
Alakesh, Alakesh
Jagirdar, Sameer Kumar
Rangarajan, Annapoorni
Jhunjhunwala, Siddharth
author_sort Pramanik, Nilkamal
collection PubMed
description [Image: see text] The antioxidant property of cerium oxide nanoparticles has increased their demand as a nanocarrier to improve the delivery and therapeutic efficacy of anticancer drugs. Here, we report the synthesis of alginate-coated ceria nanoformulations (ceria NPs) and characterization using FTIR spectroscopy, Raman microscopy, and X-ray diffraction. The synthesized ceria NPs show negligible inherent in vitro toxicity when tested on a MDA-MB-231 breast cancer cell line at higher particle concentrations. Upon loading these particles with doxorubicin (Dox) and paclitaxel (PTX) drugs, we observe a potential synergistic cytotoxic effect mediated by the drug and the ceria NPs, resulting in the better killing capacity as well as suppression of cell migration against the MDA-MB-231 cell line. Further, to verify the immune-escaping capacity before targeting cancer cells, we coated the drug-loaded ceria NPs with the membrane of MDA-MB-231 cells using an extrusion method. The resultant delivery system exhibited in vitro preferential uptake by the MDA-MB-231 cell line and showed reduced uptake by the murine macrophage cell line (RAW 264.7), assigning its potential application as non-immunogenic personalized therapy in targeting and killing of cancer cells.
format Online
Article
Text
id pubmed-9476200
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-94762002022-09-16 Surface-Coated Cerium Nanoparticles to Improve Chemotherapeutic Delivery to Tumor Cells Pramanik, Nilkamal De, Tamasa Sharma, Preeti Alakesh, Alakesh Jagirdar, Sameer Kumar Rangarajan, Annapoorni Jhunjhunwala, Siddharth ACS Omega [Image: see text] The antioxidant property of cerium oxide nanoparticles has increased their demand as a nanocarrier to improve the delivery and therapeutic efficacy of anticancer drugs. Here, we report the synthesis of alginate-coated ceria nanoformulations (ceria NPs) and characterization using FTIR spectroscopy, Raman microscopy, and X-ray diffraction. The synthesized ceria NPs show negligible inherent in vitro toxicity when tested on a MDA-MB-231 breast cancer cell line at higher particle concentrations. Upon loading these particles with doxorubicin (Dox) and paclitaxel (PTX) drugs, we observe a potential synergistic cytotoxic effect mediated by the drug and the ceria NPs, resulting in the better killing capacity as well as suppression of cell migration against the MDA-MB-231 cell line. Further, to verify the immune-escaping capacity before targeting cancer cells, we coated the drug-loaded ceria NPs with the membrane of MDA-MB-231 cells using an extrusion method. The resultant delivery system exhibited in vitro preferential uptake by the MDA-MB-231 cell line and showed reduced uptake by the murine macrophage cell line (RAW 264.7), assigning its potential application as non-immunogenic personalized therapy in targeting and killing of cancer cells. American Chemical Society 2022-09-01 /pmc/articles/PMC9476200/ /pubmed/36120021 http://dx.doi.org/10.1021/acsomega.2c00062 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Pramanik, Nilkamal
De, Tamasa
Sharma, Preeti
Alakesh, Alakesh
Jagirdar, Sameer Kumar
Rangarajan, Annapoorni
Jhunjhunwala, Siddharth
Surface-Coated Cerium Nanoparticles to Improve Chemotherapeutic Delivery to Tumor Cells
title Surface-Coated Cerium Nanoparticles to Improve Chemotherapeutic Delivery to Tumor Cells
title_full Surface-Coated Cerium Nanoparticles to Improve Chemotherapeutic Delivery to Tumor Cells
title_fullStr Surface-Coated Cerium Nanoparticles to Improve Chemotherapeutic Delivery to Tumor Cells
title_full_unstemmed Surface-Coated Cerium Nanoparticles to Improve Chemotherapeutic Delivery to Tumor Cells
title_short Surface-Coated Cerium Nanoparticles to Improve Chemotherapeutic Delivery to Tumor Cells
title_sort surface-coated cerium nanoparticles to improve chemotherapeutic delivery to tumor cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476200/
https://www.ncbi.nlm.nih.gov/pubmed/36120021
http://dx.doi.org/10.1021/acsomega.2c00062
work_keys_str_mv AT pramaniknilkamal surfacecoatedceriumnanoparticlestoimprovechemotherapeuticdeliverytotumorcells
AT detamasa surfacecoatedceriumnanoparticlestoimprovechemotherapeuticdeliverytotumorcells
AT sharmapreeti surfacecoatedceriumnanoparticlestoimprovechemotherapeuticdeliverytotumorcells
AT alakeshalakesh surfacecoatedceriumnanoparticlestoimprovechemotherapeuticdeliverytotumorcells
AT jagirdarsameerkumar surfacecoatedceriumnanoparticlestoimprovechemotherapeuticdeliverytotumorcells
AT rangarajanannapoorni surfacecoatedceriumnanoparticlestoimprovechemotherapeuticdeliverytotumorcells
AT jhunjhunwalasiddharth surfacecoatedceriumnanoparticlestoimprovechemotherapeuticdeliverytotumorcells