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Temporo-spatial variations in resistance determinants and clonality of Acinetobacter baumannii and Pseudomonas aeruginosa strains from Romanian hospitals and wastewaters
BACKGROUND: Romania is one of the European countries reporting very high antimicrobial resistance (AMR) rates and consumption of antimicrobials. We aimed to characterize the AMR profiles and clonality of 304 multi-drug resistant (MDR) Acinetobacter baumannii (Ab) and Pseudomonas aeruginosa (Pa) stra...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476303/ https://www.ncbi.nlm.nih.gov/pubmed/36104761 http://dx.doi.org/10.1186/s13756-022-01156-1 |
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author | Gheorghe-Barbu, Irina Barbu, Ilda Czobor Popa, Laura Ioana Pîrcălăbioru, Grațiela Grădișteanu Popa, Marcela Măruțescu, Luminița Niță-Lazar, Mihai Banciu, Alina Stoica, Cătălina Gheorghe, Ștefania Lucaciu, Irina Săndulescu, Oana Paraschiv, Simona Surleac, Marius Talapan, Daniela Muntean, Andrei Alexandru Preda, Mădălina Muntean, Mădălina-Maria Dragomirescu, Cristiana Cerasella Popa, Mircea Ioan Oțelea, Dan Chifiriuc, Mariana Carmen |
author_facet | Gheorghe-Barbu, Irina Barbu, Ilda Czobor Popa, Laura Ioana Pîrcălăbioru, Grațiela Grădișteanu Popa, Marcela Măruțescu, Luminița Niță-Lazar, Mihai Banciu, Alina Stoica, Cătălina Gheorghe, Ștefania Lucaciu, Irina Săndulescu, Oana Paraschiv, Simona Surleac, Marius Talapan, Daniela Muntean, Andrei Alexandru Preda, Mădălina Muntean, Mădălina-Maria Dragomirescu, Cristiana Cerasella Popa, Mircea Ioan Oțelea, Dan Chifiriuc, Mariana Carmen |
author_sort | Gheorghe-Barbu, Irina |
collection | PubMed |
description | BACKGROUND: Romania is one of the European countries reporting very high antimicrobial resistance (AMR) rates and consumption of antimicrobials. We aimed to characterize the AMR profiles and clonality of 304 multi-drug resistant (MDR) Acinetobacter baumannii (Ab) and Pseudomonas aeruginosa (Pa) strains isolated during two consecutive years (2018 and 2019) from hospital settings, hospital collecting sewage tanks and the receiving wastewater treatment plants (WWTPs) located in the main geographical regions of Romania. METHODS: The strains were isolated on chromogenic media and identified by MALDI-TOF-MS. Antibiotic susceptibility testing and confirmation of ESBL- and CP- producing phenotypes and genotypes were performed. The genetic characterization also included horizontal gene transfer experiments, whole-genome sequencing (WGS), assembling, annotation and characterization. RESULTS: Both clinical and aquatic isolates exhibited high MDR rates, especially the Ab strains isolated from nosocomial infections and hospital effluents. The phenotypic resistance profiles and MDR rates have largely varied by sampling point and geographic location. The highest MDR rates in the aquatic isolates were recorded in Galați WWTP, followed by Bucharest. The Ab strains harbored mostly bla(OXA-23), bla(OXA-24), bla(SHV), bla(TEM) and bla(GES), while Pa strains bla(IMP), bla(VIM), bla(NDM), bla(VEB), bla(GES) and bla(TEM), with high variations depending on the geographical zone and the sampling point. The WGS analysis revealed the presence of antibiotic resistance genes (ARGs) to other antibiotic classes, such as aminoglycosides, tetracyclines, sulphonamides, fosfomycin, phenicols, trimethoprim-sulfamethoxazole as well as class 1 integrons. The molecular analyses highlighted: (i) The presence of epidemic clones such as ST2 for Ab and ST233 and ST357 for Pa; (ii) The relatedness between clinical and hospital wastewater strains and (iii) The possible dissemination of clinical Ab belonging to ST2 (also proved in the conjugation assays for bla(OXA-23) or bla(OXA-72) genes), ST79 and ST492 and of Pa strains belonging to ST357, ST640 and ST621 in the wastewaters. CONCLUSION: Our study reveals the presence of CP-producing Ab and Pa in all sampling points and the clonal dissemination of clinical Ab ST2 strains in the wastewaters. The prevalent clones were correlated with the presence of class 1 integrons, suggesting that these isolates could be a significant reservoir of ARGs, being able to persist in the environment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13756-022-01156-1. |
format | Online Article Text |
id | pubmed-9476303 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-94763032022-09-16 Temporo-spatial variations in resistance determinants and clonality of Acinetobacter baumannii and Pseudomonas aeruginosa strains from Romanian hospitals and wastewaters Gheorghe-Barbu, Irina Barbu, Ilda Czobor Popa, Laura Ioana Pîrcălăbioru, Grațiela Grădișteanu Popa, Marcela Măruțescu, Luminița Niță-Lazar, Mihai Banciu, Alina Stoica, Cătălina Gheorghe, Ștefania Lucaciu, Irina Săndulescu, Oana Paraschiv, Simona Surleac, Marius Talapan, Daniela Muntean, Andrei Alexandru Preda, Mădălina Muntean, Mădălina-Maria Dragomirescu, Cristiana Cerasella Popa, Mircea Ioan Oțelea, Dan Chifiriuc, Mariana Carmen Antimicrob Resist Infect Control Research BACKGROUND: Romania is one of the European countries reporting very high antimicrobial resistance (AMR) rates and consumption of antimicrobials. We aimed to characterize the AMR profiles and clonality of 304 multi-drug resistant (MDR) Acinetobacter baumannii (Ab) and Pseudomonas aeruginosa (Pa) strains isolated during two consecutive years (2018 and 2019) from hospital settings, hospital collecting sewage tanks and the receiving wastewater treatment plants (WWTPs) located in the main geographical regions of Romania. METHODS: The strains were isolated on chromogenic media and identified by MALDI-TOF-MS. Antibiotic susceptibility testing and confirmation of ESBL- and CP- producing phenotypes and genotypes were performed. The genetic characterization also included horizontal gene transfer experiments, whole-genome sequencing (WGS), assembling, annotation and characterization. RESULTS: Both clinical and aquatic isolates exhibited high MDR rates, especially the Ab strains isolated from nosocomial infections and hospital effluents. The phenotypic resistance profiles and MDR rates have largely varied by sampling point and geographic location. The highest MDR rates in the aquatic isolates were recorded in Galați WWTP, followed by Bucharest. The Ab strains harbored mostly bla(OXA-23), bla(OXA-24), bla(SHV), bla(TEM) and bla(GES), while Pa strains bla(IMP), bla(VIM), bla(NDM), bla(VEB), bla(GES) and bla(TEM), with high variations depending on the geographical zone and the sampling point. The WGS analysis revealed the presence of antibiotic resistance genes (ARGs) to other antibiotic classes, such as aminoglycosides, tetracyclines, sulphonamides, fosfomycin, phenicols, trimethoprim-sulfamethoxazole as well as class 1 integrons. The molecular analyses highlighted: (i) The presence of epidemic clones such as ST2 for Ab and ST233 and ST357 for Pa; (ii) The relatedness between clinical and hospital wastewater strains and (iii) The possible dissemination of clinical Ab belonging to ST2 (also proved in the conjugation assays for bla(OXA-23) or bla(OXA-72) genes), ST79 and ST492 and of Pa strains belonging to ST357, ST640 and ST621 in the wastewaters. CONCLUSION: Our study reveals the presence of CP-producing Ab and Pa in all sampling points and the clonal dissemination of clinical Ab ST2 strains in the wastewaters. The prevalent clones were correlated with the presence of class 1 integrons, suggesting that these isolates could be a significant reservoir of ARGs, being able to persist in the environment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13756-022-01156-1. BioMed Central 2022-09-14 /pmc/articles/PMC9476303/ /pubmed/36104761 http://dx.doi.org/10.1186/s13756-022-01156-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Gheorghe-Barbu, Irina Barbu, Ilda Czobor Popa, Laura Ioana Pîrcălăbioru, Grațiela Grădișteanu Popa, Marcela Măruțescu, Luminița Niță-Lazar, Mihai Banciu, Alina Stoica, Cătălina Gheorghe, Ștefania Lucaciu, Irina Săndulescu, Oana Paraschiv, Simona Surleac, Marius Talapan, Daniela Muntean, Andrei Alexandru Preda, Mădălina Muntean, Mădălina-Maria Dragomirescu, Cristiana Cerasella Popa, Mircea Ioan Oțelea, Dan Chifiriuc, Mariana Carmen Temporo-spatial variations in resistance determinants and clonality of Acinetobacter baumannii and Pseudomonas aeruginosa strains from Romanian hospitals and wastewaters |
title | Temporo-spatial variations in resistance determinants and clonality of Acinetobacter baumannii and Pseudomonas aeruginosa strains from Romanian hospitals and wastewaters |
title_full | Temporo-spatial variations in resistance determinants and clonality of Acinetobacter baumannii and Pseudomonas aeruginosa strains from Romanian hospitals and wastewaters |
title_fullStr | Temporo-spatial variations in resistance determinants and clonality of Acinetobacter baumannii and Pseudomonas aeruginosa strains from Romanian hospitals and wastewaters |
title_full_unstemmed | Temporo-spatial variations in resistance determinants and clonality of Acinetobacter baumannii and Pseudomonas aeruginosa strains from Romanian hospitals and wastewaters |
title_short | Temporo-spatial variations in resistance determinants and clonality of Acinetobacter baumannii and Pseudomonas aeruginosa strains from Romanian hospitals and wastewaters |
title_sort | temporo-spatial variations in resistance determinants and clonality of acinetobacter baumannii and pseudomonas aeruginosa strains from romanian hospitals and wastewaters |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476303/ https://www.ncbi.nlm.nih.gov/pubmed/36104761 http://dx.doi.org/10.1186/s13756-022-01156-1 |
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