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Metabonomics study of liver and kidney subacute toxicity induced by garidi-5 in rats
OBJECTIVE: Metabonomics was used to analyze and explore the biomarkers and possible mechanisms of liver and kidney subacute toxicity induced by garidi-5 in rats. METHODS: Taking garidi-5 as the target drug and SD rats as the research objects, each administration group except the normal group was int...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476469/ https://www.ncbi.nlm.nih.gov/pubmed/36118012 http://dx.doi.org/10.1016/j.chmed.2022.05.003 |
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author | Wurihan Aodungerle Bilige Lili Sirguleng Aduqinfu Bai, Meirong |
author_facet | Wurihan Aodungerle Bilige Lili Sirguleng Aduqinfu Bai, Meirong |
author_sort | Wurihan |
collection | PubMed |
description | OBJECTIVE: Metabonomics was used to analyze and explore the biomarkers and possible mechanisms of liver and kidney subacute toxicity induced by garidi-5 in rats. METHODS: Taking garidi-5 as the target drug and SD rats as the research objects, each administration group except the normal group was intragastric administration of the corresponding drug solution for 28 d. The serum, liver and kidney samples of rats were detected by metabolomics and characterized by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) to identify the sensitive markers and metabolic pathways of liver and kidney subacute toxicity. RESULTS: Metabolomics analysis showed that compared with the normal group (Z), the 52, 64 and 54 different metabolites were identified in the serum, liver and kidney samples of garidi-5 high dose group (GG), which were mainly enriched in ABC transporters, arginine and proline metabolism, nicotinate and nicotinamide metabolism, central carbon metabolism in cancer pathways. CONCLUSION: The preliminarily suggested that garidi-5 can damage the liver and kidney by affecting the ABC transporters, arginine and proline metabolism, nicotinate and nicotinamide metabolism pathways, etc. Trimethylamine N-oxide, l-pyroglutamic acid, glycine-betaine, xanthine, glutathione, l-leucine, cytidine, l-arginine, spermidine, urea, 5-aminovaleric acid, creatine, l-glutamic acid, 1-methylnicotinamide and S-adenosyl-l-methionine can be used as potential biomarkers of liver and kidney toxicity sensitivity. |
format | Online Article Text |
id | pubmed-9476469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-94764692022-09-16 Metabonomics study of liver and kidney subacute toxicity induced by garidi-5 in rats Wurihan Aodungerle Bilige Lili Sirguleng Aduqinfu Bai, Meirong Chin Herb Med Original Article OBJECTIVE: Metabonomics was used to analyze and explore the biomarkers and possible mechanisms of liver and kidney subacute toxicity induced by garidi-5 in rats. METHODS: Taking garidi-5 as the target drug and SD rats as the research objects, each administration group except the normal group was intragastric administration of the corresponding drug solution for 28 d. The serum, liver and kidney samples of rats were detected by metabolomics and characterized by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) to identify the sensitive markers and metabolic pathways of liver and kidney subacute toxicity. RESULTS: Metabolomics analysis showed that compared with the normal group (Z), the 52, 64 and 54 different metabolites were identified in the serum, liver and kidney samples of garidi-5 high dose group (GG), which were mainly enriched in ABC transporters, arginine and proline metabolism, nicotinate and nicotinamide metabolism, central carbon metabolism in cancer pathways. CONCLUSION: The preliminarily suggested that garidi-5 can damage the liver and kidney by affecting the ABC transporters, arginine and proline metabolism, nicotinate and nicotinamide metabolism pathways, etc. Trimethylamine N-oxide, l-pyroglutamic acid, glycine-betaine, xanthine, glutathione, l-leucine, cytidine, l-arginine, spermidine, urea, 5-aminovaleric acid, creatine, l-glutamic acid, 1-methylnicotinamide and S-adenosyl-l-methionine can be used as potential biomarkers of liver and kidney toxicity sensitivity. Elsevier 2022-07-22 /pmc/articles/PMC9476469/ /pubmed/36118012 http://dx.doi.org/10.1016/j.chmed.2022.05.003 Text en © 2022 Tianjin Press of Chinese Herbal Medicines. Published by ELSEVIER B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Wurihan Aodungerle Bilige Lili Sirguleng Aduqinfu Bai, Meirong Metabonomics study of liver and kidney subacute toxicity induced by garidi-5 in rats |
title | Metabonomics study of liver and kidney subacute toxicity induced by garidi-5 in rats |
title_full | Metabonomics study of liver and kidney subacute toxicity induced by garidi-5 in rats |
title_fullStr | Metabonomics study of liver and kidney subacute toxicity induced by garidi-5 in rats |
title_full_unstemmed | Metabonomics study of liver and kidney subacute toxicity induced by garidi-5 in rats |
title_short | Metabonomics study of liver and kidney subacute toxicity induced by garidi-5 in rats |
title_sort | metabonomics study of liver and kidney subacute toxicity induced by garidi-5 in rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476469/ https://www.ncbi.nlm.nih.gov/pubmed/36118012 http://dx.doi.org/10.1016/j.chmed.2022.05.003 |
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