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Molecular mechanism of Chuanxiong Rhizoma in treating coronary artery diseases

OBJECTIVE: Most of the studies on the herb Chuanxiong Rhizoma (CR) have focused on the l-arginine-nitric oxide (NO) pathway, but the nitrate-nitrite-NO (NO(3)(−)–NO(2)(−)–NO) pathway was rarely investigated. Therefore, the aim of this study was to evaluate the effects and mechanisms of action of CR...

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Autores principales: Yin, Bang-qiao, Guo, Yu-hong, Liu, Yuan, Zhao, Yang-yang, Huang, Shan-mei, Wei, Xia-wei, Wang, Heng-sheng, Liu, Ruo-ya, Liu, Ying, Tang, Yao-ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476474/
https://www.ncbi.nlm.nih.gov/pubmed/36118926
http://dx.doi.org/10.1016/j.chmed.2021.03.001
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author Yin, Bang-qiao
Guo, Yu-hong
Liu, Yuan
Zhao, Yang-yang
Huang, Shan-mei
Wei, Xia-wei
Wang, Heng-sheng
Liu, Ruo-ya
Liu, Ying
Tang, Yao-ping
author_facet Yin, Bang-qiao
Guo, Yu-hong
Liu, Yuan
Zhao, Yang-yang
Huang, Shan-mei
Wei, Xia-wei
Wang, Heng-sheng
Liu, Ruo-ya
Liu, Ying
Tang, Yao-ping
author_sort Yin, Bang-qiao
collection PubMed
description OBJECTIVE: Most of the studies on the herb Chuanxiong Rhizoma (CR) have focused on the l-arginine-nitric oxide (NO) pathway, but the nitrate-nitrite-NO (NO(3)(−)–NO(2)(−)–NO) pathway was rarely investigated. Therefore, the aim of this study was to evaluate the effects and mechanisms of action of CR in coronary artery disease (CAD). METHODS: The NO(3)(−), NO(2)(−) and NO levels were examined in the NO(3)(−)–NO(2)(−)–NO pathway. High-performance ion chromatography was used to quantify NO(3)(−) and NO(2)(−) levels. Then, NO was quantified using a multifunctional enzyme marker with a fluorescent probe. The tension of aortic rings was measured using a multi myograph system. RESULTS: High content of NO(3)(−) and low content of NO(2)(−) was found in CR, and which could potently convert NO(3)(−) to NO(2)(−) in the presence of endogenous reductase enzyme. Incubating human coronary artery endothelial cells (HCAECs) with CR-containing serum showed that CR significantly decreased the NO(3)(−) content and increased the levels of NO(2)(−) and NO in the cells under hypoxic conditions. In addition, CR significantly relaxed isolated aortic rings when the l-arginine –NO pathway was blocked. The optimal concentration of CR for relaxation was 200 mg/mL. CONCLUSION: CR supplements large amounts of NO in cells and vessels to achieve relaxation via the NO(3)(−)–NO(2)(−)–NO pathway, thereby making up for the deficiency caused by the lack of NO after the l-arginine-NO pathway is suppressed. This study also supports the potential use of a traditional Chinese herb for future drug development.
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spelling pubmed-94764742022-09-16 Molecular mechanism of Chuanxiong Rhizoma in treating coronary artery diseases Yin, Bang-qiao Guo, Yu-hong Liu, Yuan Zhao, Yang-yang Huang, Shan-mei Wei, Xia-wei Wang, Heng-sheng Liu, Ruo-ya Liu, Ying Tang, Yao-ping Chin Herb Med Original Article OBJECTIVE: Most of the studies on the herb Chuanxiong Rhizoma (CR) have focused on the l-arginine-nitric oxide (NO) pathway, but the nitrate-nitrite-NO (NO(3)(−)–NO(2)(−)–NO) pathway was rarely investigated. Therefore, the aim of this study was to evaluate the effects and mechanisms of action of CR in coronary artery disease (CAD). METHODS: The NO(3)(−), NO(2)(−) and NO levels were examined in the NO(3)(−)–NO(2)(−)–NO pathway. High-performance ion chromatography was used to quantify NO(3)(−) and NO(2)(−) levels. Then, NO was quantified using a multifunctional enzyme marker with a fluorescent probe. The tension of aortic rings was measured using a multi myograph system. RESULTS: High content of NO(3)(−) and low content of NO(2)(−) was found in CR, and which could potently convert NO(3)(−) to NO(2)(−) in the presence of endogenous reductase enzyme. Incubating human coronary artery endothelial cells (HCAECs) with CR-containing serum showed that CR significantly decreased the NO(3)(−) content and increased the levels of NO(2)(−) and NO in the cells under hypoxic conditions. In addition, CR significantly relaxed isolated aortic rings when the l-arginine –NO pathway was blocked. The optimal concentration of CR for relaxation was 200 mg/mL. CONCLUSION: CR supplements large amounts of NO in cells and vessels to achieve relaxation via the NO(3)(−)–NO(2)(−)–NO pathway, thereby making up for the deficiency caused by the lack of NO after the l-arginine-NO pathway is suppressed. This study also supports the potential use of a traditional Chinese herb for future drug development. Elsevier 2021-03-17 /pmc/articles/PMC9476474/ /pubmed/36118926 http://dx.doi.org/10.1016/j.chmed.2021.03.001 Text en © 2021 Tianjin Press of Chinese Herbal Medicines. Published by ELSEVIER B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Yin, Bang-qiao
Guo, Yu-hong
Liu, Yuan
Zhao, Yang-yang
Huang, Shan-mei
Wei, Xia-wei
Wang, Heng-sheng
Liu, Ruo-ya
Liu, Ying
Tang, Yao-ping
Molecular mechanism of Chuanxiong Rhizoma in treating coronary artery diseases
title Molecular mechanism of Chuanxiong Rhizoma in treating coronary artery diseases
title_full Molecular mechanism of Chuanxiong Rhizoma in treating coronary artery diseases
title_fullStr Molecular mechanism of Chuanxiong Rhizoma in treating coronary artery diseases
title_full_unstemmed Molecular mechanism of Chuanxiong Rhizoma in treating coronary artery diseases
title_short Molecular mechanism of Chuanxiong Rhizoma in treating coronary artery diseases
title_sort molecular mechanism of chuanxiong rhizoma in treating coronary artery diseases
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476474/
https://www.ncbi.nlm.nih.gov/pubmed/36118926
http://dx.doi.org/10.1016/j.chmed.2021.03.001
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