Cargando…
Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry: A Strategy for Optimization, Characterization, and Quantification of Antioxidant Nitro Derivatives
[Image: see text] As an antioxidant, N-phenyl-β-naphthylamine (PBNA) inhibits the activity of oxidants, such as NO(x), to prevent the degradation of energetic materials. In the presence of NO(x), nitrated products can be generated in the process potentially. To characterize nitrated PBNA in a nontar...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476526/ https://www.ncbi.nlm.nih.gov/pubmed/36119998 http://dx.doi.org/10.1021/acsomega.2c04376 |
_version_ | 1784790158357299200 |
---|---|
author | Chen, Kitmin Edgar, Alexander S. Wong, Camille H. Yang, Dali |
author_facet | Chen, Kitmin Edgar, Alexander S. Wong, Camille H. Yang, Dali |
author_sort | Chen, Kitmin |
collection | PubMed |
description | [Image: see text] As an antioxidant, N-phenyl-β-naphthylamine (PBNA) inhibits the activity of oxidants, such as NO(x), to prevent the degradation of energetic materials. In the presence of NO(x), nitrated products can be generated in the process potentially. To characterize nitrated PBNA in a nontargeted analysis of complex samples as such, liquid chromatography tandem quadrupole time-of-flight (LC-QTOF), as an excellent analytic technique, is used due to its high resolution and sensitivity. However, a systematic approach of instrumentation optimization, data interpretation, and quantitative determination of products is needed. Through a step-by-step evaluation of the instrumental parameters used in the Q0, Q1, and Q2 compartments of LC-QTOF, optimal ion yields of precursor ions and high-resolution MS(2) fragmentation spectra at low mass defects were obtained in both negative and positive electrospray ionization modes. Through rationalization of the fragmentation pathways and verification using theoretical masses, the mononitro derivative of PBNA was accurately identified as N-(4-nitrophenyl)-naphthalen-2-amine and further confirmed using a reference standard. Using strict criteria provided by the analytical guidelines (e.g., SANTE), limit of quantitation, limit of detection, and calibration were established for the quantitation of PBNA and nitrated PBNA. From optimization to characterization and subsequent quantification of the mononitro-PBNA derivative, for the first time, the applicability of this strategy is demonstrated in the aged energetic binders. |
format | Online Article Text |
id | pubmed-9476526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-94765262022-09-16 Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry: A Strategy for Optimization, Characterization, and Quantification of Antioxidant Nitro Derivatives Chen, Kitmin Edgar, Alexander S. Wong, Camille H. Yang, Dali ACS Omega [Image: see text] As an antioxidant, N-phenyl-β-naphthylamine (PBNA) inhibits the activity of oxidants, such as NO(x), to prevent the degradation of energetic materials. In the presence of NO(x), nitrated products can be generated in the process potentially. To characterize nitrated PBNA in a nontargeted analysis of complex samples as such, liquid chromatography tandem quadrupole time-of-flight (LC-QTOF), as an excellent analytic technique, is used due to its high resolution and sensitivity. However, a systematic approach of instrumentation optimization, data interpretation, and quantitative determination of products is needed. Through a step-by-step evaluation of the instrumental parameters used in the Q0, Q1, and Q2 compartments of LC-QTOF, optimal ion yields of precursor ions and high-resolution MS(2) fragmentation spectra at low mass defects were obtained in both negative and positive electrospray ionization modes. Through rationalization of the fragmentation pathways and verification using theoretical masses, the mononitro derivative of PBNA was accurately identified as N-(4-nitrophenyl)-naphthalen-2-amine and further confirmed using a reference standard. Using strict criteria provided by the analytical guidelines (e.g., SANTE), limit of quantitation, limit of detection, and calibration were established for the quantitation of PBNA and nitrated PBNA. From optimization to characterization and subsequent quantification of the mononitro-PBNA derivative, for the first time, the applicability of this strategy is demonstrated in the aged energetic binders. American Chemical Society 2022-09-06 /pmc/articles/PMC9476526/ /pubmed/36119998 http://dx.doi.org/10.1021/acsomega.2c04376 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Chen, Kitmin Edgar, Alexander S. Wong, Camille H. Yang, Dali Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry: A Strategy for Optimization, Characterization, and Quantification of Antioxidant Nitro Derivatives |
title | Liquid Chromatography
Quadrupole Time-of-Flight Mass
Spectrometry: A Strategy for Optimization, Characterization, and Quantification
of Antioxidant Nitro Derivatives |
title_full | Liquid Chromatography
Quadrupole Time-of-Flight Mass
Spectrometry: A Strategy for Optimization, Characterization, and Quantification
of Antioxidant Nitro Derivatives |
title_fullStr | Liquid Chromatography
Quadrupole Time-of-Flight Mass
Spectrometry: A Strategy for Optimization, Characterization, and Quantification
of Antioxidant Nitro Derivatives |
title_full_unstemmed | Liquid Chromatography
Quadrupole Time-of-Flight Mass
Spectrometry: A Strategy for Optimization, Characterization, and Quantification
of Antioxidant Nitro Derivatives |
title_short | Liquid Chromatography
Quadrupole Time-of-Flight Mass
Spectrometry: A Strategy for Optimization, Characterization, and Quantification
of Antioxidant Nitro Derivatives |
title_sort | liquid chromatography
quadrupole time-of-flight mass
spectrometry: a strategy for optimization, characterization, and quantification
of antioxidant nitro derivatives |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476526/ https://www.ncbi.nlm.nih.gov/pubmed/36119998 http://dx.doi.org/10.1021/acsomega.2c04376 |
work_keys_str_mv | AT chenkitmin liquidchromatographyquadrupoletimeofflightmassspectrometryastrategyforoptimizationcharacterizationandquantificationofantioxidantnitroderivatives AT edgaralexanders liquidchromatographyquadrupoletimeofflightmassspectrometryastrategyforoptimizationcharacterizationandquantificationofantioxidantnitroderivatives AT wongcamilleh liquidchromatographyquadrupoletimeofflightmassspectrometryastrategyforoptimizationcharacterizationandquantificationofantioxidantnitroderivatives AT yangdali liquidchromatographyquadrupoletimeofflightmassspectrometryastrategyforoptimizationcharacterizationandquantificationofantioxidantnitroderivatives |