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Combined application of extended depth of field imaging, image stitching and polarized microscopy techniques in identification of Spatholobus suberectus
OBJECTIVE: As traditional techniques for microscopic identification of Chinese medicines currently lack objective and high-quality reference images, here we developed a systemic procedure to be used in microscopic identification of Chinese medicines, which would lead to more objective, effective and...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476799/ https://www.ncbi.nlm.nih.gov/pubmed/36120168 http://dx.doi.org/10.1016/j.chmed.2020.10.001 |
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author | Ma, Ying-xian Sutcharitchan, Chayanis Li, Xiao-die Meng, Qian Wang, Xin Ji, Shen Cui, Ya-jun |
author_facet | Ma, Ying-xian Sutcharitchan, Chayanis Li, Xiao-die Meng, Qian Wang, Xin Ji, Shen Cui, Ya-jun |
author_sort | Ma, Ying-xian |
collection | PubMed |
description | OBJECTIVE: As traditional techniques for microscopic identification of Chinese medicines currently lack objective and high-quality reference images, here we developed a systemic procedure to be used in microscopic identification of Chinese medicines, which would lead to more objective, effective and accurate identification process. METHODS: Spatholobi Caulis (Jixueteng in Chinese) was used as the specimen in the development of such procedure. Jixueteng samples were microscopically examined in bright- and dark-field microscopy. Microscopic images were obtained by regular, EDF, and image stitching techniques. RESULTS: The microscopic images of the characteristics in pulverized Jixueteng were captured, thanks to EDF imaging and image stitching techniques which allowed the detailed and full sighting of each characteristic to be obtained simultaneously. Different layers in anatomical transverse section, including cork, phelloderm, cortex, phloem, cambium, xylem and pith, were distinctively observed. Moreover, by comparing images of bright- and dark-field microscopy, birefringent and non- birefringent components could readily be distinguished. CONCLUSION: With application of the developed procedure, high-definition, panoramic and microscopic images were acquired, which could be used as the reference images for microscopic identification of Chinese medicines. |
format | Online Article Text |
id | pubmed-9476799 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-94767992022-09-16 Combined application of extended depth of field imaging, image stitching and polarized microscopy techniques in identification of Spatholobus suberectus Ma, Ying-xian Sutcharitchan, Chayanis Li, Xiao-die Meng, Qian Wang, Xin Ji, Shen Cui, Ya-jun Chin Herb Med Original Article OBJECTIVE: As traditional techniques for microscopic identification of Chinese medicines currently lack objective and high-quality reference images, here we developed a systemic procedure to be used in microscopic identification of Chinese medicines, which would lead to more objective, effective and accurate identification process. METHODS: Spatholobi Caulis (Jixueteng in Chinese) was used as the specimen in the development of such procedure. Jixueteng samples were microscopically examined in bright- and dark-field microscopy. Microscopic images were obtained by regular, EDF, and image stitching techniques. RESULTS: The microscopic images of the characteristics in pulverized Jixueteng were captured, thanks to EDF imaging and image stitching techniques which allowed the detailed and full sighting of each characteristic to be obtained simultaneously. Different layers in anatomical transverse section, including cork, phelloderm, cortex, phloem, cambium, xylem and pith, were distinctively observed. Moreover, by comparing images of bright- and dark-field microscopy, birefringent and non- birefringent components could readily be distinguished. CONCLUSION: With application of the developed procedure, high-definition, panoramic and microscopic images were acquired, which could be used as the reference images for microscopic identification of Chinese medicines. Elsevier 2020-10-16 /pmc/articles/PMC9476799/ /pubmed/36120168 http://dx.doi.org/10.1016/j.chmed.2020.10.001 Text en © 2020 Tianjin Press of Chinese Herbal Medicines. Published by ELSEVIER B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Ma, Ying-xian Sutcharitchan, Chayanis Li, Xiao-die Meng, Qian Wang, Xin Ji, Shen Cui, Ya-jun Combined application of extended depth of field imaging, image stitching and polarized microscopy techniques in identification of Spatholobus suberectus |
title | Combined application of extended depth of field imaging, image stitching and polarized microscopy techniques in identification of Spatholobus suberectus |
title_full | Combined application of extended depth of field imaging, image stitching and polarized microscopy techniques in identification of Spatholobus suberectus |
title_fullStr | Combined application of extended depth of field imaging, image stitching and polarized microscopy techniques in identification of Spatholobus suberectus |
title_full_unstemmed | Combined application of extended depth of field imaging, image stitching and polarized microscopy techniques in identification of Spatholobus suberectus |
title_short | Combined application of extended depth of field imaging, image stitching and polarized microscopy techniques in identification of Spatholobus suberectus |
title_sort | combined application of extended depth of field imaging, image stitching and polarized microscopy techniques in identification of spatholobus suberectus |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9476799/ https://www.ncbi.nlm.nih.gov/pubmed/36120168 http://dx.doi.org/10.1016/j.chmed.2020.10.001 |
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