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Collagen pre-strain discontinuity at the bone—Cartilage interface

The bone-cartilage unit (BCU) is a universal feature in diarthrodial joints, which is mechanically-graded and subjected to shear and compressive strains. Changes in the BCU have been linked to osteoarthritis (OA) progression. Here we report existence of a physiological internal strain gradient (pre-...

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Autores principales: Badar, Waqas, Ali, Husna, Brooker, Olivia N., Newham, Elis, Snow, Tim, Terrill, Nicholas J., Tozzi, Gianluca, Fratzl, Peter, Knight, Martin M., Gupta, Himadri S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9477506/
https://www.ncbi.nlm.nih.gov/pubmed/36108273
http://dx.doi.org/10.1371/journal.pone.0273832
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author Badar, Waqas
Ali, Husna
Brooker, Olivia N.
Newham, Elis
Snow, Tim
Terrill, Nicholas J.
Tozzi, Gianluca
Fratzl, Peter
Knight, Martin M.
Gupta, Himadri S.
author_facet Badar, Waqas
Ali, Husna
Brooker, Olivia N.
Newham, Elis
Snow, Tim
Terrill, Nicholas J.
Tozzi, Gianluca
Fratzl, Peter
Knight, Martin M.
Gupta, Himadri S.
author_sort Badar, Waqas
collection PubMed
description The bone-cartilage unit (BCU) is a universal feature in diarthrodial joints, which is mechanically-graded and subjected to shear and compressive strains. Changes in the BCU have been linked to osteoarthritis (OA) progression. Here we report existence of a physiological internal strain gradient (pre-strain) across the BCU at the ultrastructural scale of the extracellular matrix (ECM) constituents, specifically the collagen fibril. We use X-ray scattering that probes changes in the axial periodicity of fibril-level D-stagger of tropocollagen molecules in the matrix fibrils, as a measure of microscopic pre-strain. We find that mineralized collagen nanofibrils in the calcified plate are in tensile pre-strain relative to the underlying trabecular bone. This behaviour contrasts with the previously accepted notion that fibrillar pre-strain (or D-stagger) in collagenous tissues always reduces with mineralization, via reduced hydration and associated swelling pressure. Within the calcified part of the BCU, a finer-scale gradient in pre-strain (0.6% increase over ~50μm) is observed. The increased fibrillar pre-strain is linked to prior research reporting large tissue-level residual strains under compression. The findings may have biomechanical adaptative significance: higher in-built molecular level resilience/damage resistance to physiological compression, and disruption of the molecular-level pre-strains during remodelling of the bone-cartilage interface may be potential factors in osteoarthritis-based degeneration.
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spelling pubmed-94775062022-09-16 Collagen pre-strain discontinuity at the bone—Cartilage interface Badar, Waqas Ali, Husna Brooker, Olivia N. Newham, Elis Snow, Tim Terrill, Nicholas J. Tozzi, Gianluca Fratzl, Peter Knight, Martin M. Gupta, Himadri S. PLoS One Research Article The bone-cartilage unit (BCU) is a universal feature in diarthrodial joints, which is mechanically-graded and subjected to shear and compressive strains. Changes in the BCU have been linked to osteoarthritis (OA) progression. Here we report existence of a physiological internal strain gradient (pre-strain) across the BCU at the ultrastructural scale of the extracellular matrix (ECM) constituents, specifically the collagen fibril. We use X-ray scattering that probes changes in the axial periodicity of fibril-level D-stagger of tropocollagen molecules in the matrix fibrils, as a measure of microscopic pre-strain. We find that mineralized collagen nanofibrils in the calcified plate are in tensile pre-strain relative to the underlying trabecular bone. This behaviour contrasts with the previously accepted notion that fibrillar pre-strain (or D-stagger) in collagenous tissues always reduces with mineralization, via reduced hydration and associated swelling pressure. Within the calcified part of the BCU, a finer-scale gradient in pre-strain (0.6% increase over ~50μm) is observed. The increased fibrillar pre-strain is linked to prior research reporting large tissue-level residual strains under compression. The findings may have biomechanical adaptative significance: higher in-built molecular level resilience/damage resistance to physiological compression, and disruption of the molecular-level pre-strains during remodelling of the bone-cartilage interface may be potential factors in osteoarthritis-based degeneration. Public Library of Science 2022-09-15 /pmc/articles/PMC9477506/ /pubmed/36108273 http://dx.doi.org/10.1371/journal.pone.0273832 Text en © 2022 Badar et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Badar, Waqas
Ali, Husna
Brooker, Olivia N.
Newham, Elis
Snow, Tim
Terrill, Nicholas J.
Tozzi, Gianluca
Fratzl, Peter
Knight, Martin M.
Gupta, Himadri S.
Collagen pre-strain discontinuity at the bone—Cartilage interface
title Collagen pre-strain discontinuity at the bone—Cartilage interface
title_full Collagen pre-strain discontinuity at the bone—Cartilage interface
title_fullStr Collagen pre-strain discontinuity at the bone—Cartilage interface
title_full_unstemmed Collagen pre-strain discontinuity at the bone—Cartilage interface
title_short Collagen pre-strain discontinuity at the bone—Cartilage interface
title_sort collagen pre-strain discontinuity at the bone—cartilage interface
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9477506/
https://www.ncbi.nlm.nih.gov/pubmed/36108273
http://dx.doi.org/10.1371/journal.pone.0273832
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