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Multi-omics analysis of the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots

The regulatory effects of uneven phosphorus supplies on phosphorus transport in soybean roots are still unclear. To further analyze the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots and the effects of uneven phosphorus application on the physiological mechanism...

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Autores principales: Li, Hongyu, Xu, Letian, Li, Jiaxin, Lyu, Xiaochen, Li, Sha, Wang, Chang, Wang, Xuelai, Ma, Chunmei, Yan, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478169/
https://www.ncbi.nlm.nih.gov/pubmed/36119614
http://dx.doi.org/10.3389/fpls.2022.992036
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author Li, Hongyu
Xu, Letian
Li, Jiaxin
Lyu, Xiaochen
Li, Sha
Wang, Chang
Wang, Xuelai
Ma, Chunmei
Yan, Chao
author_facet Li, Hongyu
Xu, Letian
Li, Jiaxin
Lyu, Xiaochen
Li, Sha
Wang, Chang
Wang, Xuelai
Ma, Chunmei
Yan, Chao
author_sort Li, Hongyu
collection PubMed
description The regulatory effects of uneven phosphorus supplies on phosphorus transport in soybean roots are still unclear. To further analyze the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots and the effects of uneven phosphorus application on the physiological mechanism of phosphorus transport in soybean roots, dual-root soybean plants were prepared via grafting, and a sand culture experiment was performed. From the unfolded cotyledon stage to the initial flowering stage, one side of each dual-root soybean system was irrigated with a low-phosphorus-concentration solution (phosphorus-application [P+] side), and the other side was irrigated with a phosphorus-free nutrient solution (phosphorus-free [P-] side); this setup allowed the study of the effects of different phosphorus supply levels on the expression of genes and proteins and the accumulation of metabolites in soybean roots on the P- side to clarify the method through which phosphorus transport is regulated in soybean roots and to provide a theoretical basis for improving the use rate of phosphorus fertilizer. The results revealed that the unilateral supply of low-concentration phosphorus promoted the uptake of phosphorus by soybean roots and the transport of phosphorus from the P+ side to the P- side. Compared with the normal concentration of phosphorus supply and the phosphorus-free supply, the low concentration phosphorus supply affected the regulation of the metabolic pathways involved in starch and sucrose metabolism, glycolysis, fructose, and mannose metabolism, etc., thereby affecting soybean root phosphorus transport. The low-phosphorus stress inhibited fructose synthesis and sucrose synthase synthesis in the soybean roots and the synthesis of hexokinase (HK) and fructose kinase, which catalyzes the conversion of fructose to fructose-6-phosphate. Low-phosphorus stress promoted the synthesis of sucrose invertase and the conversion of sucrose into maltose by the activity of starch synthase (StS) and stimulated the synthesis of UDPG pyrophosphorylase (UGP) and phosphoglucose isomerase (GP1), which is involved in the conversion of UDP-glucose to glucose-6-phosphate. The phosphorus transport pathway of soybean roots was then affected, which promoted phosphorus allocation to UTP and glucose-6-phosphate. Additionally, low-phosphorus stress hastened glycolysis in the soybean roots and inhibited the synthesis of malic acid, thereby promoting the transport of phosphorus in the roots. In addition, low-phosphorus stress inhibited the synthesis of fructose, mannose, and mannose-1-phosphate and the synthesis of other enzymes involved in phosphorus transport as well as invertase, thereby inhibiting the transport and synthesis of several organic phosphorus-containing compounds.
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spelling pubmed-94781692022-09-17 Multi-omics analysis of the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots Li, Hongyu Xu, Letian Li, Jiaxin Lyu, Xiaochen Li, Sha Wang, Chang Wang, Xuelai Ma, Chunmei Yan, Chao Front Plant Sci Plant Science The regulatory effects of uneven phosphorus supplies on phosphorus transport in soybean roots are still unclear. To further analyze the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots and the effects of uneven phosphorus application on the physiological mechanism of phosphorus transport in soybean roots, dual-root soybean plants were prepared via grafting, and a sand culture experiment was performed. From the unfolded cotyledon stage to the initial flowering stage, one side of each dual-root soybean system was irrigated with a low-phosphorus-concentration solution (phosphorus-application [P+] side), and the other side was irrigated with a phosphorus-free nutrient solution (phosphorus-free [P-] side); this setup allowed the study of the effects of different phosphorus supply levels on the expression of genes and proteins and the accumulation of metabolites in soybean roots on the P- side to clarify the method through which phosphorus transport is regulated in soybean roots and to provide a theoretical basis for improving the use rate of phosphorus fertilizer. The results revealed that the unilateral supply of low-concentration phosphorus promoted the uptake of phosphorus by soybean roots and the transport of phosphorus from the P+ side to the P- side. Compared with the normal concentration of phosphorus supply and the phosphorus-free supply, the low concentration phosphorus supply affected the regulation of the metabolic pathways involved in starch and sucrose metabolism, glycolysis, fructose, and mannose metabolism, etc., thereby affecting soybean root phosphorus transport. The low-phosphorus stress inhibited fructose synthesis and sucrose synthase synthesis in the soybean roots and the synthesis of hexokinase (HK) and fructose kinase, which catalyzes the conversion of fructose to fructose-6-phosphate. Low-phosphorus stress promoted the synthesis of sucrose invertase and the conversion of sucrose into maltose by the activity of starch synthase (StS) and stimulated the synthesis of UDPG pyrophosphorylase (UGP) and phosphoglucose isomerase (GP1), which is involved in the conversion of UDP-glucose to glucose-6-phosphate. The phosphorus transport pathway of soybean roots was then affected, which promoted phosphorus allocation to UTP and glucose-6-phosphate. Additionally, low-phosphorus stress hastened glycolysis in the soybean roots and inhibited the synthesis of malic acid, thereby promoting the transport of phosphorus in the roots. In addition, low-phosphorus stress inhibited the synthesis of fructose, mannose, and mannose-1-phosphate and the synthesis of other enzymes involved in phosphorus transport as well as invertase, thereby inhibiting the transport and synthesis of several organic phosphorus-containing compounds. Frontiers Media S.A. 2022-09-02 /pmc/articles/PMC9478169/ /pubmed/36119614 http://dx.doi.org/10.3389/fpls.2022.992036 Text en Copyright © 2022 Li, Xu, Li, Lyu, Li, Wang, Wang, Ma and Yan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Li, Hongyu
Xu, Letian
Li, Jiaxin
Lyu, Xiaochen
Li, Sha
Wang, Chang
Wang, Xuelai
Ma, Chunmei
Yan, Chao
Multi-omics analysis of the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots
title Multi-omics analysis of the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots
title_full Multi-omics analysis of the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots
title_fullStr Multi-omics analysis of the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots
title_full_unstemmed Multi-omics analysis of the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots
title_short Multi-omics analysis of the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots
title_sort multi-omics analysis of the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478169/
https://www.ncbi.nlm.nih.gov/pubmed/36119614
http://dx.doi.org/10.3389/fpls.2022.992036
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