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Analysis of drug efficacy for inflammatory skin on an organ-chip system
Bacterial skin infections cause a variety of common skin diseases that require drugs that are safer than antibiotics and have fewer side effects. However, for evaluating skin disease drugs, human skin tissue in vitro constructed traditionally on Transwell has inefficient screening ability because of...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478476/ https://www.ncbi.nlm.nih.gov/pubmed/36118585 http://dx.doi.org/10.3389/fbioe.2022.939629 |
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author | Quan, Qianghua Weng, Ding Li, Xuan An, Quan Yang, Yang Yu, Bowen Ma, Yuan Wang, Jiadao |
author_facet | Quan, Qianghua Weng, Ding Li, Xuan An, Quan Yang, Yang Yu, Bowen Ma, Yuan Wang, Jiadao |
author_sort | Quan, Qianghua |
collection | PubMed |
description | Bacterial skin infections cause a variety of common skin diseases that require drugs that are safer than antibiotics and have fewer side effects. However, for evaluating skin disease drugs, human skin tissue in vitro constructed traditionally on Transwell has inefficient screening ability because of its fragile barrier function. With mechanical forces and dynamic flow, the organ-on-a-chip system became an innovative, automatic, and modular way to construct pathological models and analyze effective pharmaceutical ingredients in vitro. In this research, we integrated skin extracellular matrix and skin cells into a microfluidic chip to construct a biomimetic “interface-controlled-skin-on-chip” system (IC-SoC), which constructed a stable air–liquid interface (ALI) and necessary mechanical signals for the development of human skin equivalents. The results demonstrated that in the microfluidic system with a flowing microenvironment and ALI, the skin tissue formed in vitro could differentiate into more mature tissue morphological structures and improve barrier function. Then, following exposing the skin surface on the IC-SoC to the stimulation of Propionibacterium acnes (P.acnes) and SLS (sodium lauryl sulfate), the barrier function decreased, as well as inflammatory factors such as IL-1α, IL-8, and PEG2 increased in the medium channel of the IC-SoC. After this pathological skin model was treated with dexamethasone and polyphyllin H, the results showed that polyphyllin H had a significant repair effect on the skin barrier and a significant inhibition effect on the release of inflammation-related cytokines, and the effects were more prominent than dexamethasone. This automated microfluidic system delivers an efficient tissue model for toxicological applications and drug evaluation for bacterial-infected damaged skin instead of animals. |
format | Online Article Text |
id | pubmed-9478476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94784762022-09-17 Analysis of drug efficacy for inflammatory skin on an organ-chip system Quan, Qianghua Weng, Ding Li, Xuan An, Quan Yang, Yang Yu, Bowen Ma, Yuan Wang, Jiadao Front Bioeng Biotechnol Bioengineering and Biotechnology Bacterial skin infections cause a variety of common skin diseases that require drugs that are safer than antibiotics and have fewer side effects. However, for evaluating skin disease drugs, human skin tissue in vitro constructed traditionally on Transwell has inefficient screening ability because of its fragile barrier function. With mechanical forces and dynamic flow, the organ-on-a-chip system became an innovative, automatic, and modular way to construct pathological models and analyze effective pharmaceutical ingredients in vitro. In this research, we integrated skin extracellular matrix and skin cells into a microfluidic chip to construct a biomimetic “interface-controlled-skin-on-chip” system (IC-SoC), which constructed a stable air–liquid interface (ALI) and necessary mechanical signals for the development of human skin equivalents. The results demonstrated that in the microfluidic system with a flowing microenvironment and ALI, the skin tissue formed in vitro could differentiate into more mature tissue morphological structures and improve barrier function. Then, following exposing the skin surface on the IC-SoC to the stimulation of Propionibacterium acnes (P.acnes) and SLS (sodium lauryl sulfate), the barrier function decreased, as well as inflammatory factors such as IL-1α, IL-8, and PEG2 increased in the medium channel of the IC-SoC. After this pathological skin model was treated with dexamethasone and polyphyllin H, the results showed that polyphyllin H had a significant repair effect on the skin barrier and a significant inhibition effect on the release of inflammation-related cytokines, and the effects were more prominent than dexamethasone. This automated microfluidic system delivers an efficient tissue model for toxicological applications and drug evaluation for bacterial-infected damaged skin instead of animals. Frontiers Media S.A. 2022-09-02 /pmc/articles/PMC9478476/ /pubmed/36118585 http://dx.doi.org/10.3389/fbioe.2022.939629 Text en Copyright © 2022 Quan, Weng, Li, An, Yang, Yu, Ma and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Quan, Qianghua Weng, Ding Li, Xuan An, Quan Yang, Yang Yu, Bowen Ma, Yuan Wang, Jiadao Analysis of drug efficacy for inflammatory skin on an organ-chip system |
title | Analysis of drug efficacy for inflammatory skin on an organ-chip system |
title_full | Analysis of drug efficacy for inflammatory skin on an organ-chip system |
title_fullStr | Analysis of drug efficacy for inflammatory skin on an organ-chip system |
title_full_unstemmed | Analysis of drug efficacy for inflammatory skin on an organ-chip system |
title_short | Analysis of drug efficacy for inflammatory skin on an organ-chip system |
title_sort | analysis of drug efficacy for inflammatory skin on an organ-chip system |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478476/ https://www.ncbi.nlm.nih.gov/pubmed/36118585 http://dx.doi.org/10.3389/fbioe.2022.939629 |
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