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Analysis of drug efficacy for inflammatory skin on an organ-chip system

Bacterial skin infections cause a variety of common skin diseases that require drugs that are safer than antibiotics and have fewer side effects. However, for evaluating skin disease drugs, human skin tissue in vitro constructed traditionally on Transwell has inefficient screening ability because of...

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Autores principales: Quan, Qianghua, Weng, Ding, Li, Xuan, An, Quan, Yang, Yang, Yu, Bowen, Ma, Yuan, Wang, Jiadao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478476/
https://www.ncbi.nlm.nih.gov/pubmed/36118585
http://dx.doi.org/10.3389/fbioe.2022.939629
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author Quan, Qianghua
Weng, Ding
Li, Xuan
An, Quan
Yang, Yang
Yu, Bowen
Ma, Yuan
Wang, Jiadao
author_facet Quan, Qianghua
Weng, Ding
Li, Xuan
An, Quan
Yang, Yang
Yu, Bowen
Ma, Yuan
Wang, Jiadao
author_sort Quan, Qianghua
collection PubMed
description Bacterial skin infections cause a variety of common skin diseases that require drugs that are safer than antibiotics and have fewer side effects. However, for evaluating skin disease drugs, human skin tissue in vitro constructed traditionally on Transwell has inefficient screening ability because of its fragile barrier function. With mechanical forces and dynamic flow, the organ-on-a-chip system became an innovative, automatic, and modular way to construct pathological models and analyze effective pharmaceutical ingredients in vitro. In this research, we integrated skin extracellular matrix and skin cells into a microfluidic chip to construct a biomimetic “interface-controlled-skin-on-chip” system (IC-SoC), which constructed a stable air–liquid interface (ALI) and necessary mechanical signals for the development of human skin equivalents. The results demonstrated that in the microfluidic system with a flowing microenvironment and ALI, the skin tissue formed in vitro could differentiate into more mature tissue morphological structures and improve barrier function. Then, following exposing the skin surface on the IC-SoC to the stimulation of Propionibacterium acnes (P.acnes) and SLS (sodium lauryl sulfate), the barrier function decreased, as well as inflammatory factors such as IL-1α, IL-8, and PEG2 increased in the medium channel of the IC-SoC. After this pathological skin model was treated with dexamethasone and polyphyllin H, the results showed that polyphyllin H had a significant repair effect on the skin barrier and a significant inhibition effect on the release of inflammation-related cytokines, and the effects were more prominent than dexamethasone. This automated microfluidic system delivers an efficient tissue model for toxicological applications and drug evaluation for bacterial-infected damaged skin instead of animals.
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spelling pubmed-94784762022-09-17 Analysis of drug efficacy for inflammatory skin on an organ-chip system Quan, Qianghua Weng, Ding Li, Xuan An, Quan Yang, Yang Yu, Bowen Ma, Yuan Wang, Jiadao Front Bioeng Biotechnol Bioengineering and Biotechnology Bacterial skin infections cause a variety of common skin diseases that require drugs that are safer than antibiotics and have fewer side effects. However, for evaluating skin disease drugs, human skin tissue in vitro constructed traditionally on Transwell has inefficient screening ability because of its fragile barrier function. With mechanical forces and dynamic flow, the organ-on-a-chip system became an innovative, automatic, and modular way to construct pathological models and analyze effective pharmaceutical ingredients in vitro. In this research, we integrated skin extracellular matrix and skin cells into a microfluidic chip to construct a biomimetic “interface-controlled-skin-on-chip” system (IC-SoC), which constructed a stable air–liquid interface (ALI) and necessary mechanical signals for the development of human skin equivalents. The results demonstrated that in the microfluidic system with a flowing microenvironment and ALI, the skin tissue formed in vitro could differentiate into more mature tissue morphological structures and improve barrier function. Then, following exposing the skin surface on the IC-SoC to the stimulation of Propionibacterium acnes (P.acnes) and SLS (sodium lauryl sulfate), the barrier function decreased, as well as inflammatory factors such as IL-1α, IL-8, and PEG2 increased in the medium channel of the IC-SoC. After this pathological skin model was treated with dexamethasone and polyphyllin H, the results showed that polyphyllin H had a significant repair effect on the skin barrier and a significant inhibition effect on the release of inflammation-related cytokines, and the effects were more prominent than dexamethasone. This automated microfluidic system delivers an efficient tissue model for toxicological applications and drug evaluation for bacterial-infected damaged skin instead of animals. Frontiers Media S.A. 2022-09-02 /pmc/articles/PMC9478476/ /pubmed/36118585 http://dx.doi.org/10.3389/fbioe.2022.939629 Text en Copyright © 2022 Quan, Weng, Li, An, Yang, Yu, Ma and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Quan, Qianghua
Weng, Ding
Li, Xuan
An, Quan
Yang, Yang
Yu, Bowen
Ma, Yuan
Wang, Jiadao
Analysis of drug efficacy for inflammatory skin on an organ-chip system
title Analysis of drug efficacy for inflammatory skin on an organ-chip system
title_full Analysis of drug efficacy for inflammatory skin on an organ-chip system
title_fullStr Analysis of drug efficacy for inflammatory skin on an organ-chip system
title_full_unstemmed Analysis of drug efficacy for inflammatory skin on an organ-chip system
title_short Analysis of drug efficacy for inflammatory skin on an organ-chip system
title_sort analysis of drug efficacy for inflammatory skin on an organ-chip system
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478476/
https://www.ncbi.nlm.nih.gov/pubmed/36118585
http://dx.doi.org/10.3389/fbioe.2022.939629
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