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FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer
Accurate prediction of Bacillus Calmette–Guérin (BCG) response is essential to identify bladder cancer (BCa) patients most likely to respond sustainably, but no molecular marker predicting BCG response is available in clinical routine. Therefore, we first identified that fibroblast growth factor bin...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478507/ https://www.ncbi.nlm.nih.gov/pubmed/36119059 http://dx.doi.org/10.3389/fimmu.2022.954836 |
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author | Li, Fei Zhang, Henghui Wang, Yu Yao, Zhihao Xie, Kunfeng Mo, Qixin Fan, Qin Hou, Lina Deng, Fan Tan, Wanlong |
author_facet | Li, Fei Zhang, Henghui Wang, Yu Yao, Zhihao Xie, Kunfeng Mo, Qixin Fan, Qin Hou, Lina Deng, Fan Tan, Wanlong |
author_sort | Li, Fei |
collection | PubMed |
description | Accurate prediction of Bacillus Calmette–Guérin (BCG) response is essential to identify bladder cancer (BCa) patients most likely to respond sustainably, but no molecular marker predicting BCG response is available in clinical routine. Therefore, we first identified that fibroblast growth factor binding protein 1 (FGFBP1) was upregulated in failures of BCG therapy, and the increased FGFBP1 had a poor outcome for BCa patients in the E-MTAB-4321 and GSE19423 datasets. These different expression genes associated with FGFBP1 expression are mainly involved in neutrophil activation, neutrophil-mediated immunity, and tumor necrosis factor-mediated signal pathways in biological processes. A significant positive correlation was observed between FGFBP1 expression and regulatory T-cell (Treg) infiltration by the Spearman correlation test in the BCG cohort (r = 0.177) and The Cancer Genome Atlas (TCGA) cohort (r = 0.176), suggesting that FGFBP1 may influence the response of BCa patients to BCG immunotherapy through immune escape. Though FGFBP1 expression was positively correlated with the expressions of PD-L1, CTLA4, and PDCD1 in TCGA cohort, a strong association between FGFBP1 and PD-L1 expression was only detected in the BCG cohort (r = 0.750). Furthermore, elevated FGFBP1 was observed in BCa cell lines and tissues in comparison to corresponding normal controls by RT-qPCR, Western blotting, and immunohistochemical staining. Increased FGFBP1 was further detected in the failures than in the responders by immunohistochemical staining. Notably, FGFBP1 is positively associated with PD-L1 expression in BCa patients with BCG treatment. To sum up, FGFBP1 in BCa tissue could be identified as a promising biomarker for the accurate prediction of BCG response in BCa. |
format | Online Article Text |
id | pubmed-9478507 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94785072022-09-17 FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer Li, Fei Zhang, Henghui Wang, Yu Yao, Zhihao Xie, Kunfeng Mo, Qixin Fan, Qin Hou, Lina Deng, Fan Tan, Wanlong Front Immunol Immunology Accurate prediction of Bacillus Calmette–Guérin (BCG) response is essential to identify bladder cancer (BCa) patients most likely to respond sustainably, but no molecular marker predicting BCG response is available in clinical routine. Therefore, we first identified that fibroblast growth factor binding protein 1 (FGFBP1) was upregulated in failures of BCG therapy, and the increased FGFBP1 had a poor outcome for BCa patients in the E-MTAB-4321 and GSE19423 datasets. These different expression genes associated with FGFBP1 expression are mainly involved in neutrophil activation, neutrophil-mediated immunity, and tumor necrosis factor-mediated signal pathways in biological processes. A significant positive correlation was observed between FGFBP1 expression and regulatory T-cell (Treg) infiltration by the Spearman correlation test in the BCG cohort (r = 0.177) and The Cancer Genome Atlas (TCGA) cohort (r = 0.176), suggesting that FGFBP1 may influence the response of BCa patients to BCG immunotherapy through immune escape. Though FGFBP1 expression was positively correlated with the expressions of PD-L1, CTLA4, and PDCD1 in TCGA cohort, a strong association between FGFBP1 and PD-L1 expression was only detected in the BCG cohort (r = 0.750). Furthermore, elevated FGFBP1 was observed in BCa cell lines and tissues in comparison to corresponding normal controls by RT-qPCR, Western blotting, and immunohistochemical staining. Increased FGFBP1 was further detected in the failures than in the responders by immunohistochemical staining. Notably, FGFBP1 is positively associated with PD-L1 expression in BCa patients with BCG treatment. To sum up, FGFBP1 in BCa tissue could be identified as a promising biomarker for the accurate prediction of BCG response in BCa. Frontiers Media S.A. 2022-09-02 /pmc/articles/PMC9478507/ /pubmed/36119059 http://dx.doi.org/10.3389/fimmu.2022.954836 Text en Copyright © 2022 Li, Zhang, Wang, Yao, Xie, Mo, Fan, Hou, Deng and Tan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Li, Fei Zhang, Henghui Wang, Yu Yao, Zhihao Xie, Kunfeng Mo, Qixin Fan, Qin Hou, Lina Deng, Fan Tan, Wanlong FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer |
title | FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer |
title_full | FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer |
title_fullStr | FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer |
title_full_unstemmed | FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer |
title_short | FGFBP1 as a potential biomarker predicting bacillus Calmette–Guérin response in bladder cancer |
title_sort | fgfbp1 as a potential biomarker predicting bacillus calmette–guérin response in bladder cancer |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478507/ https://www.ncbi.nlm.nih.gov/pubmed/36119059 http://dx.doi.org/10.3389/fimmu.2022.954836 |
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