Systematic analysis and comparison of O-glycosylation of five recombinant spike proteins in β-coronaviruses

β-coronaviruses (β-CoVs), representative with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), depend on their highly glycosylated spike proteins to mediate cell entry and membrane fusion. Compared with the extensively identified N-glycosylation, less is known about O-glycosylation of β...

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Autores principales: Dong, Xuefang, Li, Xiuling, Chen, Cheng, Zhang, Xiaofei, Liang, Xinmiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478876/
https://www.ncbi.nlm.nih.gov/pubmed/36192065
http://dx.doi.org/10.1016/j.aca.2022.340394
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author Dong, Xuefang
Li, Xiuling
Chen, Cheng
Zhang, Xiaofei
Liang, Xinmiao
author_facet Dong, Xuefang
Li, Xiuling
Chen, Cheng
Zhang, Xiaofei
Liang, Xinmiao
author_sort Dong, Xuefang
collection PubMed
description β-coronaviruses (β-CoVs), representative with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), depend on their highly glycosylated spike proteins to mediate cell entry and membrane fusion. Compared with the extensively identified N-glycosylation, less is known about O-glycosylation of β-CoVs S proteins, let alone its biological functions. Herein we comprehensively characterized O-glycosylation of five recombinant β-CoVs S1 subunits and revealed the macro- and micro-heterogeneity nature of site-specific O-glycosylation. We also uncovered the O-glycosylation differences between SARS-CoV-2 and its natural D614G mutant on functional domains. This work describes the systematic O-glycosylation analysis of β-CoVs S1 proteins and will help to guide the related vaccines and antiviral drugs development.
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spelling pubmed-94788762022-09-16 Systematic analysis and comparison of O-glycosylation of five recombinant spike proteins in β-coronaviruses Dong, Xuefang Li, Xiuling Chen, Cheng Zhang, Xiaofei Liang, Xinmiao Anal Chim Acta Article β-coronaviruses (β-CoVs), representative with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), depend on their highly glycosylated spike proteins to mediate cell entry and membrane fusion. Compared with the extensively identified N-glycosylation, less is known about O-glycosylation of β-CoVs S proteins, let alone its biological functions. Herein we comprehensively characterized O-glycosylation of five recombinant β-CoVs S1 subunits and revealed the macro- and micro-heterogeneity nature of site-specific O-glycosylation. We also uncovered the O-glycosylation differences between SARS-CoV-2 and its natural D614G mutant on functional domains. This work describes the systematic O-glycosylation analysis of β-CoVs S1 proteins and will help to guide the related vaccines and antiviral drugs development. Elsevier B.V. 2022-10-16 2022-09-16 /pmc/articles/PMC9478876/ /pubmed/36192065 http://dx.doi.org/10.1016/j.aca.2022.340394 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Dong, Xuefang
Li, Xiuling
Chen, Cheng
Zhang, Xiaofei
Liang, Xinmiao
Systematic analysis and comparison of O-glycosylation of five recombinant spike proteins in β-coronaviruses
title Systematic analysis and comparison of O-glycosylation of five recombinant spike proteins in β-coronaviruses
title_full Systematic analysis and comparison of O-glycosylation of five recombinant spike proteins in β-coronaviruses
title_fullStr Systematic analysis and comparison of O-glycosylation of five recombinant spike proteins in β-coronaviruses
title_full_unstemmed Systematic analysis and comparison of O-glycosylation of five recombinant spike proteins in β-coronaviruses
title_short Systematic analysis and comparison of O-glycosylation of five recombinant spike proteins in β-coronaviruses
title_sort systematic analysis and comparison of o-glycosylation of five recombinant spike proteins in β-coronaviruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478876/
https://www.ncbi.nlm.nih.gov/pubmed/36192065
http://dx.doi.org/10.1016/j.aca.2022.340394
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