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Multimodality system of x-ray and fluorescence based on Fourier single-pixel imaging for small animals
SIGNIFICANCE: The multimodality imaging system has become a powerful tool for in-vivo biomedical research. However, a conventional multimodality system generally employs two independent detectors, which is costly and bulky. Meanwhile, the geometric cocalibration and image registration between the im...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society of Photo-Optical Instrumentation Engineers
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478903/ https://www.ncbi.nlm.nih.gov/pubmed/36114605 http://dx.doi.org/10.1117/1.JBO.27.9.090501 |
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author | Huang, Zhuoyao Zhang, Jie Gong, Hui Yang, Xiaoquan |
author_facet | Huang, Zhuoyao Zhang, Jie Gong, Hui Yang, Xiaoquan |
author_sort | Huang, Zhuoyao |
collection | PubMed |
description | SIGNIFICANCE: The multimodality imaging system has become a powerful tool for in-vivo biomedical research. However, a conventional multimodality system generally employs two independent detectors, which is costly and bulky. Meanwhile, the geometric cocalibration and image registration between the imaging modalities are also complicated. AIM: To acquire the multimodality images for small animals with only one visible light sensed single-pixel detector. APPROACH: The system is built based on a structured detection Fourier single-pixel imaging architecture. A cesium iodide doped with thallium [CsI(Tl)] scintillator plate is placed behind the sample in x-ray imaging, so the x-ray images can be converted to be visible and sensed with the same single-pixel detector as applied in fluorescence imaging. RESULTS: The spatial resolution of x-ray imaging was measured to be 1.81 mm, the sensitivity and the imaging depth of fluorescence imaging was evaluated to be [Formula: see text] and 4 mm, respectively. In vivo multimodality imaging of a C57BL/6 female mouse bearing tumor targeted with mCherry was carried out. CONCLUSIONS: We proposed an x-ray and fluorescence multimodality imaging system for small animals via the structured detection FSI architecture. The system is low cost, with a more compact structure, and free of image registration from different modalities. In vivo multimodality imaging results of a mouse bearing tumor demonstrate its capability for small animal research. |
format | Online Article Text |
id | pubmed-9478903 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Society of Photo-Optical Instrumentation Engineers |
record_format | MEDLINE/PubMed |
spelling | pubmed-94789032022-09-21 Multimodality system of x-ray and fluorescence based on Fourier single-pixel imaging for small animals Huang, Zhuoyao Zhang, Jie Gong, Hui Yang, Xiaoquan J Biomed Opt JBO Letters SIGNIFICANCE: The multimodality imaging system has become a powerful tool for in-vivo biomedical research. However, a conventional multimodality system generally employs two independent detectors, which is costly and bulky. Meanwhile, the geometric cocalibration and image registration between the imaging modalities are also complicated. AIM: To acquire the multimodality images for small animals with only one visible light sensed single-pixel detector. APPROACH: The system is built based on a structured detection Fourier single-pixel imaging architecture. A cesium iodide doped with thallium [CsI(Tl)] scintillator plate is placed behind the sample in x-ray imaging, so the x-ray images can be converted to be visible and sensed with the same single-pixel detector as applied in fluorescence imaging. RESULTS: The spatial resolution of x-ray imaging was measured to be 1.81 mm, the sensitivity and the imaging depth of fluorescence imaging was evaluated to be [Formula: see text] and 4 mm, respectively. In vivo multimodality imaging of a C57BL/6 female mouse bearing tumor targeted with mCherry was carried out. CONCLUSIONS: We proposed an x-ray and fluorescence multimodality imaging system for small animals via the structured detection FSI architecture. The system is low cost, with a more compact structure, and free of image registration from different modalities. In vivo multimodality imaging results of a mouse bearing tumor demonstrate its capability for small animal research. Society of Photo-Optical Instrumentation Engineers 2022-09-16 2022-09 /pmc/articles/PMC9478903/ /pubmed/36114605 http://dx.doi.org/10.1117/1.JBO.27.9.090501 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. |
spellingShingle | JBO Letters Huang, Zhuoyao Zhang, Jie Gong, Hui Yang, Xiaoquan Multimodality system of x-ray and fluorescence based on Fourier single-pixel imaging for small animals |
title | Multimodality system of x-ray and fluorescence based on Fourier single-pixel imaging for small animals |
title_full | Multimodality system of x-ray and fluorescence based on Fourier single-pixel imaging for small animals |
title_fullStr | Multimodality system of x-ray and fluorescence based on Fourier single-pixel imaging for small animals |
title_full_unstemmed | Multimodality system of x-ray and fluorescence based on Fourier single-pixel imaging for small animals |
title_short | Multimodality system of x-ray and fluorescence based on Fourier single-pixel imaging for small animals |
title_sort | multimodality system of x-ray and fluorescence based on fourier single-pixel imaging for small animals |
topic | JBO Letters |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478903/ https://www.ncbi.nlm.nih.gov/pubmed/36114605 http://dx.doi.org/10.1117/1.JBO.27.9.090501 |
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