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DNA Strand Displacement Driven by Host–Guest Interactions
[Image: see text] Base-pair-driven toehold-mediated strand displacement (BP-TMSD) is a fundamental concept employed for constructing DNA machines and networks with a gamut of applications—from theranostics to computational devices. To broaden the toolbox of dynamic DNA chemistry, herein, we introduc...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479067/ https://www.ncbi.nlm.nih.gov/pubmed/36063395 http://dx.doi.org/10.1021/jacs.2c05726 |
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author | Kankanamalage, Dilanka V. D. Walpita Tran, Jennifer H. T. Beltrami, Noah Meng, Kun Zhou, Xiao Pathak, Pravin Isaacs, Lyle Burin, Alexander L. Ali, Mehnaaz F. Jayawickramarajah, Janarthanan |
author_facet | Kankanamalage, Dilanka V. D. Walpita Tran, Jennifer H. T. Beltrami, Noah Meng, Kun Zhou, Xiao Pathak, Pravin Isaacs, Lyle Burin, Alexander L. Ali, Mehnaaz F. Jayawickramarajah, Janarthanan |
author_sort | Kankanamalage, Dilanka V. D. Walpita |
collection | PubMed |
description | [Image: see text] Base-pair-driven toehold-mediated strand displacement (BP-TMSD) is a fundamental concept employed for constructing DNA machines and networks with a gamut of applications—from theranostics to computational devices. To broaden the toolbox of dynamic DNA chemistry, herein, we introduce a synthetic surrogate termed host–guest-driven toehold-mediated strand displacement (HG-TMSD) that utilizes bioorthogonal, cucurbit[7]uril (CB[7]) interactions with guest-linked input sequences. Since control of the strand-displacement process is salient, we demonstrate how HG-TMSD can be finely modulated via changes to the structure of the input sequence (including synthetic guest head-group and/or linker length). Further, for a given input sequence, competing small-molecule guests can serve as effective regulators (with fine and coarse control) of HG-TMSD. To show integration into functional devices, we have incorporated HG-TMSD into machines that control enzyme activity and layered reactions that detect specific microRNA. |
format | Online Article Text |
id | pubmed-9479067 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-94790672022-09-17 DNA Strand Displacement Driven by Host–Guest Interactions Kankanamalage, Dilanka V. D. Walpita Tran, Jennifer H. T. Beltrami, Noah Meng, Kun Zhou, Xiao Pathak, Pravin Isaacs, Lyle Burin, Alexander L. Ali, Mehnaaz F. Jayawickramarajah, Janarthanan J Am Chem Soc [Image: see text] Base-pair-driven toehold-mediated strand displacement (BP-TMSD) is a fundamental concept employed for constructing DNA machines and networks with a gamut of applications—from theranostics to computational devices. To broaden the toolbox of dynamic DNA chemistry, herein, we introduce a synthetic surrogate termed host–guest-driven toehold-mediated strand displacement (HG-TMSD) that utilizes bioorthogonal, cucurbit[7]uril (CB[7]) interactions with guest-linked input sequences. Since control of the strand-displacement process is salient, we demonstrate how HG-TMSD can be finely modulated via changes to the structure of the input sequence (including synthetic guest head-group and/or linker length). Further, for a given input sequence, competing small-molecule guests can serve as effective regulators (with fine and coarse control) of HG-TMSD. To show integration into functional devices, we have incorporated HG-TMSD into machines that control enzyme activity and layered reactions that detect specific microRNA. American Chemical Society 2022-09-05 2022-09-14 /pmc/articles/PMC9479067/ /pubmed/36063395 http://dx.doi.org/10.1021/jacs.2c05726 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Kankanamalage, Dilanka V. D. Walpita Tran, Jennifer H. T. Beltrami, Noah Meng, Kun Zhou, Xiao Pathak, Pravin Isaacs, Lyle Burin, Alexander L. Ali, Mehnaaz F. Jayawickramarajah, Janarthanan DNA Strand Displacement Driven by Host–Guest Interactions |
title | DNA Strand Displacement
Driven by Host–Guest
Interactions |
title_full | DNA Strand Displacement
Driven by Host–Guest
Interactions |
title_fullStr | DNA Strand Displacement
Driven by Host–Guest
Interactions |
title_full_unstemmed | DNA Strand Displacement
Driven by Host–Guest
Interactions |
title_short | DNA Strand Displacement
Driven by Host–Guest
Interactions |
title_sort | dna strand displacement
driven by host–guest
interactions |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479067/ https://www.ncbi.nlm.nih.gov/pubmed/36063395 http://dx.doi.org/10.1021/jacs.2c05726 |
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