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Using machine learning to link the influence of transferred Agrobacterium rhizogenes genes to the hormone profile and morphological traits in Centella asiatica hairy roots
Hairy roots are made after the integration of a small set of genes from Agrobacterium rhizogenes in the plant genome. Little is known about how this small set is linked to their hormone profile, which determines development, morphology, and levels of secondary metabolite production. We used C. asiat...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479193/ https://www.ncbi.nlm.nih.gov/pubmed/36119596 http://dx.doi.org/10.3389/fpls.2022.1001023 |
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author | Alcalde, Miguel Angel Müller, Maren Munné-Bosch, Sergi Landín, Mariana Gallego, Pedro Pablo Bonfill, Mercedes Palazon, Javier Hidalgo-Martinez, Diego |
author_facet | Alcalde, Miguel Angel Müller, Maren Munné-Bosch, Sergi Landín, Mariana Gallego, Pedro Pablo Bonfill, Mercedes Palazon, Javier Hidalgo-Martinez, Diego |
author_sort | Alcalde, Miguel Angel |
collection | PubMed |
description | Hairy roots are made after the integration of a small set of genes from Agrobacterium rhizogenes in the plant genome. Little is known about how this small set is linked to their hormone profile, which determines development, morphology, and levels of secondary metabolite production. We used C. asiatica hairy root line cultures to determine the putative links between the rol and aux gene expressions with morphological traits, a hormone profile, and centelloside production. The results obtained after 14 and 28 days of culture were processed via multivariate analysis and machine-learning processes such as random forest, supported vector machines, linear discriminant analysis, and neural networks. This allowed us to obtain models capable of discriminating highly productive root lines from their levels of genetic expression (rol and aux genes) or from their hormone profile. In total, 12 hormones were evaluated, resulting in 10 being satisfactorily detected. Within this set of hormones, abscisic acid (ABA) and cytokinin isopentenyl adenosine (IPA) were found to be critical in defining the morphological traits and centelloside content. The results showed that IPA brings more benefits to the biotechnological platform. Additionally, we determined the degree of influence of each of the evaluated genes on the individual hormone profile, finding that aux1 has a significant influence on the IPA profile, while the rol genes are closely linked to the ABA profile. Finally, we effectively verified the gene influence on these two specific hormones through feeding experiments that aimed to reverse the effect on root morphology and centelloside content. |
format | Online Article Text |
id | pubmed-9479193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94791932022-09-17 Using machine learning to link the influence of transferred Agrobacterium rhizogenes genes to the hormone profile and morphological traits in Centella asiatica hairy roots Alcalde, Miguel Angel Müller, Maren Munné-Bosch, Sergi Landín, Mariana Gallego, Pedro Pablo Bonfill, Mercedes Palazon, Javier Hidalgo-Martinez, Diego Front Plant Sci Plant Science Hairy roots are made after the integration of a small set of genes from Agrobacterium rhizogenes in the plant genome. Little is known about how this small set is linked to their hormone profile, which determines development, morphology, and levels of secondary metabolite production. We used C. asiatica hairy root line cultures to determine the putative links between the rol and aux gene expressions with morphological traits, a hormone profile, and centelloside production. The results obtained after 14 and 28 days of culture were processed via multivariate analysis and machine-learning processes such as random forest, supported vector machines, linear discriminant analysis, and neural networks. This allowed us to obtain models capable of discriminating highly productive root lines from their levels of genetic expression (rol and aux genes) or from their hormone profile. In total, 12 hormones were evaluated, resulting in 10 being satisfactorily detected. Within this set of hormones, abscisic acid (ABA) and cytokinin isopentenyl adenosine (IPA) were found to be critical in defining the morphological traits and centelloside content. The results showed that IPA brings more benefits to the biotechnological platform. Additionally, we determined the degree of influence of each of the evaluated genes on the individual hormone profile, finding that aux1 has a significant influence on the IPA profile, while the rol genes are closely linked to the ABA profile. Finally, we effectively verified the gene influence on these two specific hormones through feeding experiments that aimed to reverse the effect on root morphology and centelloside content. Frontiers Media S.A. 2022-09-02 /pmc/articles/PMC9479193/ /pubmed/36119596 http://dx.doi.org/10.3389/fpls.2022.1001023 Text en Copyright © 2022 Alcalde, Müller, Munné-Bosch, Landín, Gallego, Bonfill, Palazon and Hidalgo-Martinez. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Alcalde, Miguel Angel Müller, Maren Munné-Bosch, Sergi Landín, Mariana Gallego, Pedro Pablo Bonfill, Mercedes Palazon, Javier Hidalgo-Martinez, Diego Using machine learning to link the influence of transferred Agrobacterium rhizogenes genes to the hormone profile and morphological traits in Centella asiatica hairy roots |
title | Using machine learning to link the influence of transferred Agrobacterium rhizogenes genes to the hormone profile and morphological traits in Centella asiatica hairy roots |
title_full | Using machine learning to link the influence of transferred Agrobacterium rhizogenes genes to the hormone profile and morphological traits in Centella asiatica hairy roots |
title_fullStr | Using machine learning to link the influence of transferred Agrobacterium rhizogenes genes to the hormone profile and morphological traits in Centella asiatica hairy roots |
title_full_unstemmed | Using machine learning to link the influence of transferred Agrobacterium rhizogenes genes to the hormone profile and morphological traits in Centella asiatica hairy roots |
title_short | Using machine learning to link the influence of transferred Agrobacterium rhizogenes genes to the hormone profile and morphological traits in Centella asiatica hairy roots |
title_sort | using machine learning to link the influence of transferred agrobacterium rhizogenes genes to the hormone profile and morphological traits in centella asiatica hairy roots |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479193/ https://www.ncbi.nlm.nih.gov/pubmed/36119596 http://dx.doi.org/10.3389/fpls.2022.1001023 |
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