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Near-Infrared Carbon Nanotube Tracking Reveals the Nanoscale Extracellular Space around Synapses

[Image: see text] We provide evidence of a local synaptic nanoenvironment in the brain extracellular space (ECS) lying within 500 nm of postsynaptic densities. To reveal this brain compartment, we developed a correlative imaging approach dedicated to thick brain tissue based on single-particle track...

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Detalles Bibliográficos
Autores principales: Paviolo, Chiara, Ferreira, Joana S., Lee, Antony, Hunter, Daniel, Calaresu, Ivo, Nandi, Somen, Groc, Laurent, Cognet, Laurent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479209/
https://www.ncbi.nlm.nih.gov/pubmed/36038137
http://dx.doi.org/10.1021/acs.nanolett.1c04259
Descripción
Sumario:[Image: see text] We provide evidence of a local synaptic nanoenvironment in the brain extracellular space (ECS) lying within 500 nm of postsynaptic densities. To reveal this brain compartment, we developed a correlative imaging approach dedicated to thick brain tissue based on single-particle tracking of individual fluorescent single wall carbon nanotubes (SWCNTs) in living samples and on speckle-based HiLo microscopy of synaptic labels. We show that the extracellular space around synapses bears specific properties in terms of morphology at the nanoscale and inner diffusivity. We finally show that the ECS juxta-synaptic region changes its diffusion parameters in response to neuronal activity, indicating that this nanoenvironment might play a role in the regulation of brain activity.