Cargando…
The “whole ingredients extract” of Astragali Radix improves the symptoms of dextran sulfate sodium-induced ulcerative colitis in mice through systemic immunomodulation
BACKGROUND: Ulcerative colitis (UC) is a common inflammatory intestinal disease. Astragali Radix (AR) is one of the traditional Chinese medicines used in clinic for UC treatment. In our previous study, the whole ingredient extract (WIE) from AR have been proved to possess better immunomodulatory eff...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479389/ https://www.ncbi.nlm.nih.gov/pubmed/36109750 http://dx.doi.org/10.1186/s13020-022-00661-0 |
_version_ | 1784790779742388224 |
---|---|
author | Linghu, Ke-Gang Ma, Qiushuo Xiong, Shi-Hang Zhao, Mingming Chen, Qiling Xu, Wen Chen, Meiwan Zhang, Jian-Ye Hu, Yuanjia Xu, Wei Yu, Hua |
author_facet | Linghu, Ke-Gang Ma, Qiushuo Xiong, Shi-Hang Zhao, Mingming Chen, Qiling Xu, Wen Chen, Meiwan Zhang, Jian-Ye Hu, Yuanjia Xu, Wei Yu, Hua |
author_sort | Linghu, Ke-Gang |
collection | PubMed |
description | BACKGROUND: Ulcerative colitis (UC) is a common inflammatory intestinal disease. Astragali Radix (AR) is one of the traditional Chinese medicines used in clinic for UC treatment. In our previous study, the whole ingredient extract (WIE) from AR have been proved to possess better immunomodulatory effects on immunosuppressed mice compared with the conventional water extraction (WAE). In the present study, we further evaluated the therapeutic effects of WIE against dextran sodium sulfate (DSS)-induced UC in mice through systemic immune regulation. METHODS: Gradient solvent extraction has been used to prepare the WIE of AR. The HPLC–MS analysis approach has been employed to analyze and compare the chemical differences between WAE and WIE. UC model was reproduced in 3% DSS-induced C57BL/6 mice for 6 days. Flow cytometric analysis for splenic lymphocyte subset. ELISA kits were used to determine the cytokines in the serum and colon tissues. The histopathological characteristics of colon were evaluated by hematoxylin–eosin staining and immunohistochemistry. RESULTS: The chemical compositions and the contents of main active ingredients were more abundant and higher in WIE than those in WAE. The WIE treatment altered a better action on reducing colitis disease activity index (DAI) and histological scores, as well as the recovered body weight and increased colon length in mice compared to the WAE group. Additionally, WIE showed better effects in recovering the levels of peripheral white blood cells in blood and cytokines (IL-2, IL-6 and MCP-1) in serum or colon tissues, improving the percentage of CD3(+) and the ratio of CD4(+)/CD8(+) in the spleen, and inhibiting the spleen enlargement in DSS-induced UC mice. CONCLUSIONS: WIE has a more complete chemical composition than WAE. Meanwhile, WIE possesses better therapeutic effects on UC through resuming dysfunctional immunity in mice. |
format | Online Article Text |
id | pubmed-9479389 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-94793892022-09-17 The “whole ingredients extract” of Astragali Radix improves the symptoms of dextran sulfate sodium-induced ulcerative colitis in mice through systemic immunomodulation Linghu, Ke-Gang Ma, Qiushuo Xiong, Shi-Hang Zhao, Mingming Chen, Qiling Xu, Wen Chen, Meiwan Zhang, Jian-Ye Hu, Yuanjia Xu, Wei Yu, Hua Chin Med Research BACKGROUND: Ulcerative colitis (UC) is a common inflammatory intestinal disease. Astragali Radix (AR) is one of the traditional Chinese medicines used in clinic for UC treatment. In our previous study, the whole ingredient extract (WIE) from AR have been proved to possess better immunomodulatory effects on immunosuppressed mice compared with the conventional water extraction (WAE). In the present study, we further evaluated the therapeutic effects of WIE against dextran sodium sulfate (DSS)-induced UC in mice through systemic immune regulation. METHODS: Gradient solvent extraction has been used to prepare the WIE of AR. The HPLC–MS analysis approach has been employed to analyze and compare the chemical differences between WAE and WIE. UC model was reproduced in 3% DSS-induced C57BL/6 mice for 6 days. Flow cytometric analysis for splenic lymphocyte subset. ELISA kits were used to determine the cytokines in the serum and colon tissues. The histopathological characteristics of colon were evaluated by hematoxylin–eosin staining and immunohistochemistry. RESULTS: The chemical compositions and the contents of main active ingredients were more abundant and higher in WIE than those in WAE. The WIE treatment altered a better action on reducing colitis disease activity index (DAI) and histological scores, as well as the recovered body weight and increased colon length in mice compared to the WAE group. Additionally, WIE showed better effects in recovering the levels of peripheral white blood cells in blood and cytokines (IL-2, IL-6 and MCP-1) in serum or colon tissues, improving the percentage of CD3(+) and the ratio of CD4(+)/CD8(+) in the spleen, and inhibiting the spleen enlargement in DSS-induced UC mice. CONCLUSIONS: WIE has a more complete chemical composition than WAE. Meanwhile, WIE possesses better therapeutic effects on UC through resuming dysfunctional immunity in mice. BioMed Central 2022-09-15 /pmc/articles/PMC9479389/ /pubmed/36109750 http://dx.doi.org/10.1186/s13020-022-00661-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Linghu, Ke-Gang Ma, Qiushuo Xiong, Shi-Hang Zhao, Mingming Chen, Qiling Xu, Wen Chen, Meiwan Zhang, Jian-Ye Hu, Yuanjia Xu, Wei Yu, Hua The “whole ingredients extract” of Astragali Radix improves the symptoms of dextran sulfate sodium-induced ulcerative colitis in mice through systemic immunomodulation |
title | The “whole ingredients extract” of Astragali Radix improves the symptoms of dextran sulfate sodium-induced ulcerative colitis in mice through systemic immunomodulation |
title_full | The “whole ingredients extract” of Astragali Radix improves the symptoms of dextran sulfate sodium-induced ulcerative colitis in mice through systemic immunomodulation |
title_fullStr | The “whole ingredients extract” of Astragali Radix improves the symptoms of dextran sulfate sodium-induced ulcerative colitis in mice through systemic immunomodulation |
title_full_unstemmed | The “whole ingredients extract” of Astragali Radix improves the symptoms of dextran sulfate sodium-induced ulcerative colitis in mice through systemic immunomodulation |
title_short | The “whole ingredients extract” of Astragali Radix improves the symptoms of dextran sulfate sodium-induced ulcerative colitis in mice through systemic immunomodulation |
title_sort | “whole ingredients extract” of astragali radix improves the symptoms of dextran sulfate sodium-induced ulcerative colitis in mice through systemic immunomodulation |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479389/ https://www.ncbi.nlm.nih.gov/pubmed/36109750 http://dx.doi.org/10.1186/s13020-022-00661-0 |
work_keys_str_mv | AT linghukegang thewholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT maqiushuo thewholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT xiongshihang thewholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT zhaomingming thewholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT chenqiling thewholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT xuwen thewholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT chenmeiwan thewholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT zhangjianye thewholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT huyuanjia thewholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT xuwei thewholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT yuhua thewholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT linghukegang wholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT maqiushuo wholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT xiongshihang wholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT zhaomingming wholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT chenqiling wholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT xuwen wholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT chenmeiwan wholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT zhangjianye wholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT huyuanjia wholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT xuwei wholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation AT yuhua wholeingredientsextractofastragaliradiximprovesthesymptomsofdextransulfatesodiuminducedulcerativecolitisinmicethroughsystemicimmunomodulation |