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Mesenchymal stem cells- derived exosomes inhibit the expression of Aquaporin-5 and EGFR in HCT-116 human colorectal carcinoma cell line

BACKGROUND: Aquaporins are channel proteins, form pores in the membrane of biological cells to facilitate the transcellular and transepithelial water movement. The role of Aquaporins in carcinogenesis has become an area of interest. In this study, we aimed to investigate the effects of adipose-deriv...

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Autores principales: Mansourabadi, Amir Hossein, Aghamajidi, Azin, Faraji, Fatemeh, Taghizadeh, Shirin, Mohamed Khosroshahi, Leila, Bahramkiya, Mona, Azimi, Maryam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479423/
https://www.ncbi.nlm.nih.gov/pubmed/36114463
http://dx.doi.org/10.1186/s12860-022-00439-0
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author Mansourabadi, Amir Hossein
Aghamajidi, Azin
Faraji, Fatemeh
Taghizadeh, Shirin
Mohamed Khosroshahi, Leila
Bahramkiya, Mona
Azimi, Maryam
author_facet Mansourabadi, Amir Hossein
Aghamajidi, Azin
Faraji, Fatemeh
Taghizadeh, Shirin
Mohamed Khosroshahi, Leila
Bahramkiya, Mona
Azimi, Maryam
author_sort Mansourabadi, Amir Hossein
collection PubMed
description BACKGROUND: Aquaporins are channel proteins, form pores in the membrane of biological cells to facilitate the transcellular and transepithelial water movement. The role of Aquaporins in carcinogenesis has become an area of interest. In this study, we aimed to investigate the effects of adipose-derived mesenchymal stem cells secreted exosomes on the expression of aquaporin 5 and EGFR genes in the HCT-116 tumor cell line. METHODS AND RESULTS: Surface antigenic profile of Ad-MSCs was evaluated using specific markers. Exosomes were purified from the Ad-MSc supernatant while the quality and the shape of isolated exosomes were assessed by western blot and transmission electron microscopy (TEM) respectively. HCT-116 cells were co-cultured with MSC-conditioned medium (MSC-CM) and/or with 100 μg/ml of MSC-derived exosomes for 48 h and. Real-time PCR was carried out to determine the expression of aquaporin5 and EGFR in HCT-116. Relative expression levels were calculated using the 2(-ΔΔct) method. Our result showed that AQP5 and EGFR mRNA levels were significantly reduced in CM and/or exosomes treated HCT116 compare to the control group (P-value < 0.05). CONCLUSION: The current study showed that MSC derived exosomes could inhibit expression of two important molecules involved in tumor progression. Hence it seems MSCs-derived exosomes may hold a hopeful future as drug delivery vehicles which need the furtherer investigation.
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spelling pubmed-94794232022-09-17 Mesenchymal stem cells- derived exosomes inhibit the expression of Aquaporin-5 and EGFR in HCT-116 human colorectal carcinoma cell line Mansourabadi, Amir Hossein Aghamajidi, Azin Faraji, Fatemeh Taghizadeh, Shirin Mohamed Khosroshahi, Leila Bahramkiya, Mona Azimi, Maryam BMC Mol Cell Biol Research BACKGROUND: Aquaporins are channel proteins, form pores in the membrane of biological cells to facilitate the transcellular and transepithelial water movement. The role of Aquaporins in carcinogenesis has become an area of interest. In this study, we aimed to investigate the effects of adipose-derived mesenchymal stem cells secreted exosomes on the expression of aquaporin 5 and EGFR genes in the HCT-116 tumor cell line. METHODS AND RESULTS: Surface antigenic profile of Ad-MSCs was evaluated using specific markers. Exosomes were purified from the Ad-MSc supernatant while the quality and the shape of isolated exosomes were assessed by western blot and transmission electron microscopy (TEM) respectively. HCT-116 cells were co-cultured with MSC-conditioned medium (MSC-CM) and/or with 100 μg/ml of MSC-derived exosomes for 48 h and. Real-time PCR was carried out to determine the expression of aquaporin5 and EGFR in HCT-116. Relative expression levels were calculated using the 2(-ΔΔct) method. Our result showed that AQP5 and EGFR mRNA levels were significantly reduced in CM and/or exosomes treated HCT116 compare to the control group (P-value < 0.05). CONCLUSION: The current study showed that MSC derived exosomes could inhibit expression of two important molecules involved in tumor progression. Hence it seems MSCs-derived exosomes may hold a hopeful future as drug delivery vehicles which need the furtherer investigation. BioMed Central 2022-09-16 /pmc/articles/PMC9479423/ /pubmed/36114463 http://dx.doi.org/10.1186/s12860-022-00439-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Mansourabadi, Amir Hossein
Aghamajidi, Azin
Faraji, Fatemeh
Taghizadeh, Shirin
Mohamed Khosroshahi, Leila
Bahramkiya, Mona
Azimi, Maryam
Mesenchymal stem cells- derived exosomes inhibit the expression of Aquaporin-5 and EGFR in HCT-116 human colorectal carcinoma cell line
title Mesenchymal stem cells- derived exosomes inhibit the expression of Aquaporin-5 and EGFR in HCT-116 human colorectal carcinoma cell line
title_full Mesenchymal stem cells- derived exosomes inhibit the expression of Aquaporin-5 and EGFR in HCT-116 human colorectal carcinoma cell line
title_fullStr Mesenchymal stem cells- derived exosomes inhibit the expression of Aquaporin-5 and EGFR in HCT-116 human colorectal carcinoma cell line
title_full_unstemmed Mesenchymal stem cells- derived exosomes inhibit the expression of Aquaporin-5 and EGFR in HCT-116 human colorectal carcinoma cell line
title_short Mesenchymal stem cells- derived exosomes inhibit the expression of Aquaporin-5 and EGFR in HCT-116 human colorectal carcinoma cell line
title_sort mesenchymal stem cells- derived exosomes inhibit the expression of aquaporin-5 and egfr in hct-116 human colorectal carcinoma cell line
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479423/
https://www.ncbi.nlm.nih.gov/pubmed/36114463
http://dx.doi.org/10.1186/s12860-022-00439-0
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