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Rhodotorula benthica culture as an alternative to antibiotics improves growth performance by improving nutrients digestibility and intestinal morphology, and modulating gut microbiota of weaned piglets

The effects of Rhodotorula benthica culture (RBC) and antibiotics (AB) on the growth performance, nutrients digestibility, morphological indicators, and colonic microbiota of weaning piglets were explored. Ninety-six (Duroc × Landrace × Large) weaned piglets (21-day-old) weighing 7.7 ± 0.83 kg, were...

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Autores principales: Zhang, Qianqian, Li, Jian, Yi, Xin, Li, Zipeng, Liang, Shuang, Fang, Zhengfeng, Lin, Yan, Xu, Shengyu, Feng, Bin, Zhuo, Yong, Wu, De, Che, Lianqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479635/
https://www.ncbi.nlm.nih.gov/pubmed/36118236
http://dx.doi.org/10.3389/fmicb.2022.964531
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author Zhang, Qianqian
Li, Jian
Yi, Xin
Li, Zipeng
Liang, Shuang
Fang, Zhengfeng
Lin, Yan
Xu, Shengyu
Feng, Bin
Zhuo, Yong
Wu, De
Che, Lianqiang
author_facet Zhang, Qianqian
Li, Jian
Yi, Xin
Li, Zipeng
Liang, Shuang
Fang, Zhengfeng
Lin, Yan
Xu, Shengyu
Feng, Bin
Zhuo, Yong
Wu, De
Che, Lianqiang
author_sort Zhang, Qianqian
collection PubMed
description The effects of Rhodotorula benthica culture (RBC) and antibiotics (AB) on the growth performance, nutrients digestibility, morphological indicators, and colonic microbiota of weaning piglets were explored. Ninety-six (Duroc × Landrace × Large) weaned piglets (21-day-old) weighing 7.7 ± 0.83 kg, were randomly allocated to 4 dietary treatments. They were fed with basal diet (CON), basal diet + 25 mg/kg bacitracin zinc + 5 mg/kg colistin sulfate (AB), 5 g/kg reduction in soybean meal of basal diet + 5 g/kg RBC (RBC1), or 10 g/kg reduction in soybean meal of basal diet + 10 g/kg RBC (RBC2). The results showed that dietary RBC1 improved the body gain/feed intake (G/F) of weaned piglets than the CON diet, and the RBC2 diet improved the average daily gain and G/F than CON and AB diets from days 15 to 28 (P < 0.05). Supplementation of RBC2 improved the apparent total tract digestibility of dry matter, nitrogen, and gross energy in weaned piglets compared to controls from days 15 to 28 (P < 0.05). Dietary AB, RBC1, and RBC2 enhanced the ileal villus height (VH) and VH/crypt depth (CD), and these two indicators were greater in the RBC2-treated piglets than in the AB- and RBC1-treated piglets (P < 0.05). The activity of serum superoxide dismutase (SOD) was enhanced by dietary AB, RBC1, and RBC2 (P < 0.05). Serum glutathione (GSH) concentration was elevated by dietary RBC1 and RBC2 (P < 0.05). According to 16S rRNA sequence analysis, AB- and RBC2-treated piglets had a higher relative abundance of Firmicutes and Lachnospiraceae in the colon digesta, and more abundant Lactobacillus was found in RBC1-treated piglets, as compared to the CON group. Additionally, RBC2 supplementation increased the α diversity [Chao1, PD-whole-tree, and observed operational taxonomic units (OTUs)] compared to the CON group. Taken together, the dietary RBC improved the growth performance of weaned piglets. In addition, 10 g/kg of RBC2 in the diet achieved better effects on higher ADG, ileal villi morphology, and stronger antioxidant capacity than dietary AB and RBC1 in weaning piglets.
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spelling pubmed-94796352022-09-17 Rhodotorula benthica culture as an alternative to antibiotics improves growth performance by improving nutrients digestibility and intestinal morphology, and modulating gut microbiota of weaned piglets Zhang, Qianqian Li, Jian Yi, Xin Li, Zipeng Liang, Shuang Fang, Zhengfeng Lin, Yan Xu, Shengyu Feng, Bin Zhuo, Yong Wu, De Che, Lianqiang Front Microbiol Microbiology The effects of Rhodotorula benthica culture (RBC) and antibiotics (AB) on the growth performance, nutrients digestibility, morphological indicators, and colonic microbiota of weaning piglets were explored. Ninety-six (Duroc × Landrace × Large) weaned piglets (21-day-old) weighing 7.7 ± 0.83 kg, were randomly allocated to 4 dietary treatments. They were fed with basal diet (CON), basal diet + 25 mg/kg bacitracin zinc + 5 mg/kg colistin sulfate (AB), 5 g/kg reduction in soybean meal of basal diet + 5 g/kg RBC (RBC1), or 10 g/kg reduction in soybean meal of basal diet + 10 g/kg RBC (RBC2). The results showed that dietary RBC1 improved the body gain/feed intake (G/F) of weaned piglets than the CON diet, and the RBC2 diet improved the average daily gain and G/F than CON and AB diets from days 15 to 28 (P < 0.05). Supplementation of RBC2 improved the apparent total tract digestibility of dry matter, nitrogen, and gross energy in weaned piglets compared to controls from days 15 to 28 (P < 0.05). Dietary AB, RBC1, and RBC2 enhanced the ileal villus height (VH) and VH/crypt depth (CD), and these two indicators were greater in the RBC2-treated piglets than in the AB- and RBC1-treated piglets (P < 0.05). The activity of serum superoxide dismutase (SOD) was enhanced by dietary AB, RBC1, and RBC2 (P < 0.05). Serum glutathione (GSH) concentration was elevated by dietary RBC1 and RBC2 (P < 0.05). According to 16S rRNA sequence analysis, AB- and RBC2-treated piglets had a higher relative abundance of Firmicutes and Lachnospiraceae in the colon digesta, and more abundant Lactobacillus was found in RBC1-treated piglets, as compared to the CON group. Additionally, RBC2 supplementation increased the α diversity [Chao1, PD-whole-tree, and observed operational taxonomic units (OTUs)] compared to the CON group. Taken together, the dietary RBC improved the growth performance of weaned piglets. In addition, 10 g/kg of RBC2 in the diet achieved better effects on higher ADG, ileal villi morphology, and stronger antioxidant capacity than dietary AB and RBC1 in weaning piglets. Frontiers Media S.A. 2022-09-02 /pmc/articles/PMC9479635/ /pubmed/36118236 http://dx.doi.org/10.3389/fmicb.2022.964531 Text en Copyright © 2022 Zhang, Li, Yi, Li, Liang, Fang, Lin, Xu, Feng, Zhuo, Wu and Che. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhang, Qianqian
Li, Jian
Yi, Xin
Li, Zipeng
Liang, Shuang
Fang, Zhengfeng
Lin, Yan
Xu, Shengyu
Feng, Bin
Zhuo, Yong
Wu, De
Che, Lianqiang
Rhodotorula benthica culture as an alternative to antibiotics improves growth performance by improving nutrients digestibility and intestinal morphology, and modulating gut microbiota of weaned piglets
title Rhodotorula benthica culture as an alternative to antibiotics improves growth performance by improving nutrients digestibility and intestinal morphology, and modulating gut microbiota of weaned piglets
title_full Rhodotorula benthica culture as an alternative to antibiotics improves growth performance by improving nutrients digestibility and intestinal morphology, and modulating gut microbiota of weaned piglets
title_fullStr Rhodotorula benthica culture as an alternative to antibiotics improves growth performance by improving nutrients digestibility and intestinal morphology, and modulating gut microbiota of weaned piglets
title_full_unstemmed Rhodotorula benthica culture as an alternative to antibiotics improves growth performance by improving nutrients digestibility and intestinal morphology, and modulating gut microbiota of weaned piglets
title_short Rhodotorula benthica culture as an alternative to antibiotics improves growth performance by improving nutrients digestibility and intestinal morphology, and modulating gut microbiota of weaned piglets
title_sort rhodotorula benthica culture as an alternative to antibiotics improves growth performance by improving nutrients digestibility and intestinal morphology, and modulating gut microbiota of weaned piglets
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479635/
https://www.ncbi.nlm.nih.gov/pubmed/36118236
http://dx.doi.org/10.3389/fmicb.2022.964531
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