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Detection of a novel Pestivirus strain in Java ticks (Amblyomma javanense) and the hosts Malayan pangolin (Manis javanica) and Chinese pangolin (Manis pentadactyla)

Pangolins are endangered animals and are listed in the CITES Appendix I of the Convention International Trade Endangered Species of Wild Fauna and Flora as well as being the national first-level protected wild animal in China. Based on a few reports on pangolins infected with pestiviruses of the Fla...

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Autores principales: Shi, Yuan-Ni, Li, Lin-Miao, Zhou, Jia-Bin, Hua, Yan, Zeng, Zhi-Liao, Yu, Ye-Pin, Liu, Ping, Yuan, Zi-Guo, Chen, Jin-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479695/
https://www.ncbi.nlm.nih.gov/pubmed/36118205
http://dx.doi.org/10.3389/fmicb.2022.988730
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author Shi, Yuan-Ni
Li, Lin-Miao
Zhou, Jia-Bin
Hua, Yan
Zeng, Zhi-Liao
Yu, Ye-Pin
Liu, Ping
Yuan, Zi-Guo
Chen, Jin-Ping
author_facet Shi, Yuan-Ni
Li, Lin-Miao
Zhou, Jia-Bin
Hua, Yan
Zeng, Zhi-Liao
Yu, Ye-Pin
Liu, Ping
Yuan, Zi-Guo
Chen, Jin-Ping
author_sort Shi, Yuan-Ni
collection PubMed
description Pangolins are endangered animals and are listed in the CITES Appendix I of the Convention International Trade Endangered Species of Wild Fauna and Flora as well as being the national first-level protected wild animal in China. Based on a few reports on pangolins infected with pestiviruses of the Flaviviridae family, Pestivirus infections in pangolins have attracted increasing attention. Pangolin pestivirus is a pathogen that may cause diseases such as acute diarrhea and acute hemorrhagic syndrome. To better understand the epidemiology and genomic characterization of pestiviruses carried by pangolins, we detected pestiviruses in dead Malayan pangolin using metavirome sequencing technology and obtained a Pestivirus sequence of 12,333 nucleotides (named Guangdong pangolin Pestivirus, GDPV). Phylogenetic tree analysis based on the entire coding sequence, NS3 gene or RdRp gene sequences, showed that GDPV was closely related to previously reported pangolin-derived Pestivirus and clustered into a separate branch. Molecular epidemiological investigation revealed that 15 Pestivirus-positive tissues from two pangolins individuals with a positivity rate of 5.56%, and six Amblyomma javanense carried pestiviruses with a positivity rate of 19.35%. Moreover, the RdRp gene of the Pestivirus carried by A. javanense showed a high similarity to that carried by pangolins (93–100%), indicating A. javanense is likely to represent the vector of Pestivirus transmission. This study expands the diversity of viruses carried by pangolins and provides an important reference value for interrupting the transmission route of the virus and protecting the health of pangolins.
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spelling pubmed-94796952022-09-17 Detection of a novel Pestivirus strain in Java ticks (Amblyomma javanense) and the hosts Malayan pangolin (Manis javanica) and Chinese pangolin (Manis pentadactyla) Shi, Yuan-Ni Li, Lin-Miao Zhou, Jia-Bin Hua, Yan Zeng, Zhi-Liao Yu, Ye-Pin Liu, Ping Yuan, Zi-Guo Chen, Jin-Ping Front Microbiol Microbiology Pangolins are endangered animals and are listed in the CITES Appendix I of the Convention International Trade Endangered Species of Wild Fauna and Flora as well as being the national first-level protected wild animal in China. Based on a few reports on pangolins infected with pestiviruses of the Flaviviridae family, Pestivirus infections in pangolins have attracted increasing attention. Pangolin pestivirus is a pathogen that may cause diseases such as acute diarrhea and acute hemorrhagic syndrome. To better understand the epidemiology and genomic characterization of pestiviruses carried by pangolins, we detected pestiviruses in dead Malayan pangolin using metavirome sequencing technology and obtained a Pestivirus sequence of 12,333 nucleotides (named Guangdong pangolin Pestivirus, GDPV). Phylogenetic tree analysis based on the entire coding sequence, NS3 gene or RdRp gene sequences, showed that GDPV was closely related to previously reported pangolin-derived Pestivirus and clustered into a separate branch. Molecular epidemiological investigation revealed that 15 Pestivirus-positive tissues from two pangolins individuals with a positivity rate of 5.56%, and six Amblyomma javanense carried pestiviruses with a positivity rate of 19.35%. Moreover, the RdRp gene of the Pestivirus carried by A. javanense showed a high similarity to that carried by pangolins (93–100%), indicating A. javanense is likely to represent the vector of Pestivirus transmission. This study expands the diversity of viruses carried by pangolins and provides an important reference value for interrupting the transmission route of the virus and protecting the health of pangolins. Frontiers Media S.A. 2022-09-02 /pmc/articles/PMC9479695/ /pubmed/36118205 http://dx.doi.org/10.3389/fmicb.2022.988730 Text en Copyright © 2022 Shi, Li, Zhou, Hua, Zeng, Yu, Liu, Yuan and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Shi, Yuan-Ni
Li, Lin-Miao
Zhou, Jia-Bin
Hua, Yan
Zeng, Zhi-Liao
Yu, Ye-Pin
Liu, Ping
Yuan, Zi-Guo
Chen, Jin-Ping
Detection of a novel Pestivirus strain in Java ticks (Amblyomma javanense) and the hosts Malayan pangolin (Manis javanica) and Chinese pangolin (Manis pentadactyla)
title Detection of a novel Pestivirus strain in Java ticks (Amblyomma javanense) and the hosts Malayan pangolin (Manis javanica) and Chinese pangolin (Manis pentadactyla)
title_full Detection of a novel Pestivirus strain in Java ticks (Amblyomma javanense) and the hosts Malayan pangolin (Manis javanica) and Chinese pangolin (Manis pentadactyla)
title_fullStr Detection of a novel Pestivirus strain in Java ticks (Amblyomma javanense) and the hosts Malayan pangolin (Manis javanica) and Chinese pangolin (Manis pentadactyla)
title_full_unstemmed Detection of a novel Pestivirus strain in Java ticks (Amblyomma javanense) and the hosts Malayan pangolin (Manis javanica) and Chinese pangolin (Manis pentadactyla)
title_short Detection of a novel Pestivirus strain in Java ticks (Amblyomma javanense) and the hosts Malayan pangolin (Manis javanica) and Chinese pangolin (Manis pentadactyla)
title_sort detection of a novel pestivirus strain in java ticks (amblyomma javanense) and the hosts malayan pangolin (manis javanica) and chinese pangolin (manis pentadactyla)
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479695/
https://www.ncbi.nlm.nih.gov/pubmed/36118205
http://dx.doi.org/10.3389/fmicb.2022.988730
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