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Chromosomal-level genome of velvet bean (Mucuna pruriens) provides resources for L-DOPA synthetic research and development

Mucuna pruriens, commonly called velvet bean, is the main natural source of levodopa (L-DOPA), which has been marketed as a psychoactive drug for the clinical management of Parkinson’s disease and dopamine-responsive dystonia. Although velvet bean is a very important plant species for food and pharm...

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Autores principales: Hao, Shijie, Ge, Qijin, Shao, Yunchang, Tang, Benqin, Fan, Guangyi, Qiu, Canyu, Wu, Xue, Li, Liangwei, Liu, Xiaochuan, Shi, Chengcheng, Lee, Simon Ming-Yuen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479889/
https://www.ncbi.nlm.nih.gov/pubmed/35980175
http://dx.doi.org/10.1093/dnares/dsac031
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author Hao, Shijie
Ge, Qijin
Shao, Yunchang
Tang, Benqin
Fan, Guangyi
Qiu, Canyu
Wu, Xue
Li, Liangwei
Liu, Xiaochuan
Shi, Chengcheng
Lee, Simon Ming-Yuen
author_facet Hao, Shijie
Ge, Qijin
Shao, Yunchang
Tang, Benqin
Fan, Guangyi
Qiu, Canyu
Wu, Xue
Li, Liangwei
Liu, Xiaochuan
Shi, Chengcheng
Lee, Simon Ming-Yuen
author_sort Hao, Shijie
collection PubMed
description Mucuna pruriens, commonly called velvet bean, is the main natural source of levodopa (L-DOPA), which has been marketed as a psychoactive drug for the clinical management of Parkinson’s disease and dopamine-responsive dystonia. Although velvet bean is a very important plant species for food and pharmaceutical manufacturing, the lack of genetic and genomic information about this species severely hinders further molecular research thereon and biotechnological development. Here, we reported the first velvet bean genome, with a size of 500.49 Mb and 11 chromosomes encoding 28,010 proteins. Genomic comparison among legume species indicated that velvet bean speciated ∼29 Ma from soybean clade, without specific genome duplication. Importantly, we identified 21 polyphenol oxidase coding genes that catalyse l-tyrosine to L-DOPA in velvet bean, and two subfamilies showing tandem expansion on Chr3 and Chr7 after speciation. Interestingly, disease-resistant and anti-pathogen gene families were found contracted in velvet bean, which might be related to the expansion of polyphenol oxidase. Our study generated a high-quality genomic reference for velvet bean, an economically important agricultural and medicinal plant, and the newly reported L-DOPA biosynthetic genes could provide indispensable information for the biotechnological and sustainable development of an environment-friendly L-DOPA biosynthesis processing method.
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spelling pubmed-94798892022-09-20 Chromosomal-level genome of velvet bean (Mucuna pruriens) provides resources for L-DOPA synthetic research and development Hao, Shijie Ge, Qijin Shao, Yunchang Tang, Benqin Fan, Guangyi Qiu, Canyu Wu, Xue Li, Liangwei Liu, Xiaochuan Shi, Chengcheng Lee, Simon Ming-Yuen DNA Res Resource Article: Genomes Explored Mucuna pruriens, commonly called velvet bean, is the main natural source of levodopa (L-DOPA), which has been marketed as a psychoactive drug for the clinical management of Parkinson’s disease and dopamine-responsive dystonia. Although velvet bean is a very important plant species for food and pharmaceutical manufacturing, the lack of genetic and genomic information about this species severely hinders further molecular research thereon and biotechnological development. Here, we reported the first velvet bean genome, with a size of 500.49 Mb and 11 chromosomes encoding 28,010 proteins. Genomic comparison among legume species indicated that velvet bean speciated ∼29 Ma from soybean clade, without specific genome duplication. Importantly, we identified 21 polyphenol oxidase coding genes that catalyse l-tyrosine to L-DOPA in velvet bean, and two subfamilies showing tandem expansion on Chr3 and Chr7 after speciation. Interestingly, disease-resistant and anti-pathogen gene families were found contracted in velvet bean, which might be related to the expansion of polyphenol oxidase. Our study generated a high-quality genomic reference for velvet bean, an economically important agricultural and medicinal plant, and the newly reported L-DOPA biosynthetic genes could provide indispensable information for the biotechnological and sustainable development of an environment-friendly L-DOPA biosynthesis processing method. Oxford University Press 2022-08-18 /pmc/articles/PMC9479889/ /pubmed/35980175 http://dx.doi.org/10.1093/dnares/dsac031 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Resource Article: Genomes Explored
Hao, Shijie
Ge, Qijin
Shao, Yunchang
Tang, Benqin
Fan, Guangyi
Qiu, Canyu
Wu, Xue
Li, Liangwei
Liu, Xiaochuan
Shi, Chengcheng
Lee, Simon Ming-Yuen
Chromosomal-level genome of velvet bean (Mucuna pruriens) provides resources for L-DOPA synthetic research and development
title Chromosomal-level genome of velvet bean (Mucuna pruriens) provides resources for L-DOPA synthetic research and development
title_full Chromosomal-level genome of velvet bean (Mucuna pruriens) provides resources for L-DOPA synthetic research and development
title_fullStr Chromosomal-level genome of velvet bean (Mucuna pruriens) provides resources for L-DOPA synthetic research and development
title_full_unstemmed Chromosomal-level genome of velvet bean (Mucuna pruriens) provides resources for L-DOPA synthetic research and development
title_short Chromosomal-level genome of velvet bean (Mucuna pruriens) provides resources for L-DOPA synthetic research and development
title_sort chromosomal-level genome of velvet bean (mucuna pruriens) provides resources for l-dopa synthetic research and development
topic Resource Article: Genomes Explored
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479889/
https://www.ncbi.nlm.nih.gov/pubmed/35980175
http://dx.doi.org/10.1093/dnares/dsac031
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