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Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering

Current tissue engineering techniques frequently rely on hydrogels to support cell growth, as these materials strongly mimic the extracellular matrix. However, hydrogels often need ad hoc customization to generate specific tissue constructs. One popular strategy for hydrogel functionalization is to...

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Autores principales: González-Gamboa, Ivonne, Velázquez-Lam, Edith, Lobo-Zegers, Matías José, Frías-Sánchez, Ada Itzel, Tavares-Negrete, Jorge Alfonso, Monroy-Borrego, Andrea, Menchaca-Arrendondo, Jorge Luis, Williams, Laura, Lunello, Pablo, Ponz, Fernando, Alvarez, Mario Moisés, Trujillo-de Santiago, Grissel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480610/
https://www.ncbi.nlm.nih.gov/pubmed/36118588
http://dx.doi.org/10.3389/fbioe.2022.907601
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author González-Gamboa, Ivonne
Velázquez-Lam, Edith
Lobo-Zegers, Matías José
Frías-Sánchez, Ada Itzel
Tavares-Negrete, Jorge Alfonso
Monroy-Borrego, Andrea
Menchaca-Arrendondo, Jorge Luis
Williams, Laura
Lunello, Pablo
Ponz, Fernando
Alvarez, Mario Moisés
Trujillo-de Santiago, Grissel
author_facet González-Gamboa, Ivonne
Velázquez-Lam, Edith
Lobo-Zegers, Matías José
Frías-Sánchez, Ada Itzel
Tavares-Negrete, Jorge Alfonso
Monroy-Borrego, Andrea
Menchaca-Arrendondo, Jorge Luis
Williams, Laura
Lunello, Pablo
Ponz, Fernando
Alvarez, Mario Moisés
Trujillo-de Santiago, Grissel
author_sort González-Gamboa, Ivonne
collection PubMed
description Current tissue engineering techniques frequently rely on hydrogels to support cell growth, as these materials strongly mimic the extracellular matrix. However, hydrogels often need ad hoc customization to generate specific tissue constructs. One popular strategy for hydrogel functionalization is to add nanoparticles to them. Here, we present a plant viral nanoparticle the turnip mosaic virus (TuMV), as a promising additive for gelatin methacryloyl (GelMA) hydrogels for the engineering of mammalian tissues. TuMV is a flexuous, elongated, tubular protein nanoparticle (700–750 nm long and 12–15 nm wide) and is incapable of infecting mammalian cells. These flexuous nanoparticles spontaneously form entangled nanomeshes in aqueous environments, and we hypothesized that this nanomesh structure could serve as a nanoscaffold for cells. Human fibroblasts loaded into GelMA-TuMV hydrogels exhibited similar metabolic activity to that of cells loaded in pristine GelMA hydrogels. However, cells cultured in GelMA-TuMV formed clusters and assumed an elongated morphology in contrast to the homogeneous and confluent cultures seen on GelMA surfaces, suggesting that the nanoscaffold material per se did not favor cell adhesion. We also covalently conjugated TuMV particles with epidermal growth factor (EGF) using a straightforward reaction scheme based on a Staudinger reaction. BJ cells cultured on the functionalized scaffolds increased their confluency by approximately 30% compared to growth with unconjugated EGF. We also provide examples of the use of GelMA-TuMV hydrogels in different biofabrication scenarios, include casting, flow-based-manufacture of filaments, and bioprinting. We envision TuMV as a versatile nanobiomaterial that can be useful for tissue engineering.
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spelling pubmed-94806102022-09-17 Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering González-Gamboa, Ivonne Velázquez-Lam, Edith Lobo-Zegers, Matías José Frías-Sánchez, Ada Itzel Tavares-Negrete, Jorge Alfonso Monroy-Borrego, Andrea Menchaca-Arrendondo, Jorge Luis Williams, Laura Lunello, Pablo Ponz, Fernando Alvarez, Mario Moisés Trujillo-de Santiago, Grissel Front Bioeng Biotechnol Bioengineering and Biotechnology Current tissue engineering techniques frequently rely on hydrogels to support cell growth, as these materials strongly mimic the extracellular matrix. However, hydrogels often need ad hoc customization to generate specific tissue constructs. One popular strategy for hydrogel functionalization is to add nanoparticles to them. Here, we present a plant viral nanoparticle the turnip mosaic virus (TuMV), as a promising additive for gelatin methacryloyl (GelMA) hydrogels for the engineering of mammalian tissues. TuMV is a flexuous, elongated, tubular protein nanoparticle (700–750 nm long and 12–15 nm wide) and is incapable of infecting mammalian cells. These flexuous nanoparticles spontaneously form entangled nanomeshes in aqueous environments, and we hypothesized that this nanomesh structure could serve as a nanoscaffold for cells. Human fibroblasts loaded into GelMA-TuMV hydrogels exhibited similar metabolic activity to that of cells loaded in pristine GelMA hydrogels. However, cells cultured in GelMA-TuMV formed clusters and assumed an elongated morphology in contrast to the homogeneous and confluent cultures seen on GelMA surfaces, suggesting that the nanoscaffold material per se did not favor cell adhesion. We also covalently conjugated TuMV particles with epidermal growth factor (EGF) using a straightforward reaction scheme based on a Staudinger reaction. BJ cells cultured on the functionalized scaffolds increased their confluency by approximately 30% compared to growth with unconjugated EGF. We also provide examples of the use of GelMA-TuMV hydrogels in different biofabrication scenarios, include casting, flow-based-manufacture of filaments, and bioprinting. We envision TuMV as a versatile nanobiomaterial that can be useful for tissue engineering. Frontiers Media S.A. 2022-09-02 /pmc/articles/PMC9480610/ /pubmed/36118588 http://dx.doi.org/10.3389/fbioe.2022.907601 Text en Copyright © 2022 González-Gamboa, Velázquez-Lam, Lobo-Zegers, Frías-Sánchez, Tavares-Negrete, Monroy-Borrego, Menchaca-Arrendondo, Williams, Lunello, Ponz, Alvarez and Trujillo-de Santiago. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
González-Gamboa, Ivonne
Velázquez-Lam, Edith
Lobo-Zegers, Matías José
Frías-Sánchez, Ada Itzel
Tavares-Negrete, Jorge Alfonso
Monroy-Borrego, Andrea
Menchaca-Arrendondo, Jorge Luis
Williams, Laura
Lunello, Pablo
Ponz, Fernando
Alvarez, Mario Moisés
Trujillo-de Santiago, Grissel
Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering
title Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering
title_full Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering
title_fullStr Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering
title_full_unstemmed Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering
title_short Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering
title_sort gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480610/
https://www.ncbi.nlm.nih.gov/pubmed/36118588
http://dx.doi.org/10.3389/fbioe.2022.907601
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