Cargando…
Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering
Current tissue engineering techniques frequently rely on hydrogels to support cell growth, as these materials strongly mimic the extracellular matrix. However, hydrogels often need ad hoc customization to generate specific tissue constructs. One popular strategy for hydrogel functionalization is to...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480610/ https://www.ncbi.nlm.nih.gov/pubmed/36118588 http://dx.doi.org/10.3389/fbioe.2022.907601 |
_version_ | 1784791074359738368 |
---|---|
author | González-Gamboa, Ivonne Velázquez-Lam, Edith Lobo-Zegers, Matías José Frías-Sánchez, Ada Itzel Tavares-Negrete, Jorge Alfonso Monroy-Borrego, Andrea Menchaca-Arrendondo, Jorge Luis Williams, Laura Lunello, Pablo Ponz, Fernando Alvarez, Mario Moisés Trujillo-de Santiago, Grissel |
author_facet | González-Gamboa, Ivonne Velázquez-Lam, Edith Lobo-Zegers, Matías José Frías-Sánchez, Ada Itzel Tavares-Negrete, Jorge Alfonso Monroy-Borrego, Andrea Menchaca-Arrendondo, Jorge Luis Williams, Laura Lunello, Pablo Ponz, Fernando Alvarez, Mario Moisés Trujillo-de Santiago, Grissel |
author_sort | González-Gamboa, Ivonne |
collection | PubMed |
description | Current tissue engineering techniques frequently rely on hydrogels to support cell growth, as these materials strongly mimic the extracellular matrix. However, hydrogels often need ad hoc customization to generate specific tissue constructs. One popular strategy for hydrogel functionalization is to add nanoparticles to them. Here, we present a plant viral nanoparticle the turnip mosaic virus (TuMV), as a promising additive for gelatin methacryloyl (GelMA) hydrogels for the engineering of mammalian tissues. TuMV is a flexuous, elongated, tubular protein nanoparticle (700–750 nm long and 12–15 nm wide) and is incapable of infecting mammalian cells. These flexuous nanoparticles spontaneously form entangled nanomeshes in aqueous environments, and we hypothesized that this nanomesh structure could serve as a nanoscaffold for cells. Human fibroblasts loaded into GelMA-TuMV hydrogels exhibited similar metabolic activity to that of cells loaded in pristine GelMA hydrogels. However, cells cultured in GelMA-TuMV formed clusters and assumed an elongated morphology in contrast to the homogeneous and confluent cultures seen on GelMA surfaces, suggesting that the nanoscaffold material per se did not favor cell adhesion. We also covalently conjugated TuMV particles with epidermal growth factor (EGF) using a straightforward reaction scheme based on a Staudinger reaction. BJ cells cultured on the functionalized scaffolds increased their confluency by approximately 30% compared to growth with unconjugated EGF. We also provide examples of the use of GelMA-TuMV hydrogels in different biofabrication scenarios, include casting, flow-based-manufacture of filaments, and bioprinting. We envision TuMV as a versatile nanobiomaterial that can be useful for tissue engineering. |
format | Online Article Text |
id | pubmed-9480610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94806102022-09-17 Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering González-Gamboa, Ivonne Velázquez-Lam, Edith Lobo-Zegers, Matías José Frías-Sánchez, Ada Itzel Tavares-Negrete, Jorge Alfonso Monroy-Borrego, Andrea Menchaca-Arrendondo, Jorge Luis Williams, Laura Lunello, Pablo Ponz, Fernando Alvarez, Mario Moisés Trujillo-de Santiago, Grissel Front Bioeng Biotechnol Bioengineering and Biotechnology Current tissue engineering techniques frequently rely on hydrogels to support cell growth, as these materials strongly mimic the extracellular matrix. However, hydrogels often need ad hoc customization to generate specific tissue constructs. One popular strategy for hydrogel functionalization is to add nanoparticles to them. Here, we present a plant viral nanoparticle the turnip mosaic virus (TuMV), as a promising additive for gelatin methacryloyl (GelMA) hydrogels for the engineering of mammalian tissues. TuMV is a flexuous, elongated, tubular protein nanoparticle (700–750 nm long and 12–15 nm wide) and is incapable of infecting mammalian cells. These flexuous nanoparticles spontaneously form entangled nanomeshes in aqueous environments, and we hypothesized that this nanomesh structure could serve as a nanoscaffold for cells. Human fibroblasts loaded into GelMA-TuMV hydrogels exhibited similar metabolic activity to that of cells loaded in pristine GelMA hydrogels. However, cells cultured in GelMA-TuMV formed clusters and assumed an elongated morphology in contrast to the homogeneous and confluent cultures seen on GelMA surfaces, suggesting that the nanoscaffold material per se did not favor cell adhesion. We also covalently conjugated TuMV particles with epidermal growth factor (EGF) using a straightforward reaction scheme based on a Staudinger reaction. BJ cells cultured on the functionalized scaffolds increased their confluency by approximately 30% compared to growth with unconjugated EGF. We also provide examples of the use of GelMA-TuMV hydrogels in different biofabrication scenarios, include casting, flow-based-manufacture of filaments, and bioprinting. We envision TuMV as a versatile nanobiomaterial that can be useful for tissue engineering. Frontiers Media S.A. 2022-09-02 /pmc/articles/PMC9480610/ /pubmed/36118588 http://dx.doi.org/10.3389/fbioe.2022.907601 Text en Copyright © 2022 González-Gamboa, Velázquez-Lam, Lobo-Zegers, Frías-Sánchez, Tavares-Negrete, Monroy-Borrego, Menchaca-Arrendondo, Williams, Lunello, Ponz, Alvarez and Trujillo-de Santiago. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology González-Gamboa, Ivonne Velázquez-Lam, Edith Lobo-Zegers, Matías José Frías-Sánchez, Ada Itzel Tavares-Negrete, Jorge Alfonso Monroy-Borrego, Andrea Menchaca-Arrendondo, Jorge Luis Williams, Laura Lunello, Pablo Ponz, Fernando Alvarez, Mario Moisés Trujillo-de Santiago, Grissel Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering |
title | Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering |
title_full | Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering |
title_fullStr | Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering |
title_full_unstemmed | Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering |
title_short | Gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering |
title_sort | gelatin-methacryloyl hydrogels containing turnip mosaic virus for fabrication of nanostructured materials for tissue engineering |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480610/ https://www.ncbi.nlm.nih.gov/pubmed/36118588 http://dx.doi.org/10.3389/fbioe.2022.907601 |
work_keys_str_mv | AT gonzalezgamboaivonne gelatinmethacryloylhydrogelscontainingturnipmosaicvirusforfabricationofnanostructuredmaterialsfortissueengineering AT velazquezlamedith gelatinmethacryloylhydrogelscontainingturnipmosaicvirusforfabricationofnanostructuredmaterialsfortissueengineering AT lobozegersmatiasjose gelatinmethacryloylhydrogelscontainingturnipmosaicvirusforfabricationofnanostructuredmaterialsfortissueengineering AT friassanchezadaitzel gelatinmethacryloylhydrogelscontainingturnipmosaicvirusforfabricationofnanostructuredmaterialsfortissueengineering AT tavaresnegretejorgealfonso gelatinmethacryloylhydrogelscontainingturnipmosaicvirusforfabricationofnanostructuredmaterialsfortissueengineering AT monroyborregoandrea gelatinmethacryloylhydrogelscontainingturnipmosaicvirusforfabricationofnanostructuredmaterialsfortissueengineering AT menchacaarrendondojorgeluis gelatinmethacryloylhydrogelscontainingturnipmosaicvirusforfabricationofnanostructuredmaterialsfortissueengineering AT williamslaura gelatinmethacryloylhydrogelscontainingturnipmosaicvirusforfabricationofnanostructuredmaterialsfortissueengineering AT lunellopablo gelatinmethacryloylhydrogelscontainingturnipmosaicvirusforfabricationofnanostructuredmaterialsfortissueengineering AT ponzfernando gelatinmethacryloylhydrogelscontainingturnipmosaicvirusforfabricationofnanostructuredmaterialsfortissueengineering AT alvarezmariomoises gelatinmethacryloylhydrogelscontainingturnipmosaicvirusforfabricationofnanostructuredmaterialsfortissueengineering AT trujillodesantiagogrissel gelatinmethacryloylhydrogelscontainingturnipmosaicvirusforfabricationofnanostructuredmaterialsfortissueengineering |