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A mathematical model for strigolactone biosynthesis in plants

Strigolactones mediate plant development, trigger symbiosis with arbuscular mycorrhizal fungi, are abundant in 80% of the plant kingdom and help plants gain resistance to environmental stressors. They also induce germination of parasitic plant seeds that are endemic to various continents, such as Or...

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Autores principales: Lucido, Abel, Basallo, Oriol, Sorribas, Albert, Marin-Sanguino, Alberto, Vilaprinyo, Ester, Alves, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480829/
https://www.ncbi.nlm.nih.gov/pubmed/36119618
http://dx.doi.org/10.3389/fpls.2022.979162
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author Lucido, Abel
Basallo, Oriol
Sorribas, Albert
Marin-Sanguino, Alberto
Vilaprinyo, Ester
Alves, Rui
author_facet Lucido, Abel
Basallo, Oriol
Sorribas, Albert
Marin-Sanguino, Alberto
Vilaprinyo, Ester
Alves, Rui
author_sort Lucido, Abel
collection PubMed
description Strigolactones mediate plant development, trigger symbiosis with arbuscular mycorrhizal fungi, are abundant in 80% of the plant kingdom and help plants gain resistance to environmental stressors. They also induce germination of parasitic plant seeds that are endemic to various continents, such as Orobanche in Europe or Asia and Striga in Africa. The genes involved in the early stages of strigolactones biosynthesis are known in several plants. The regulatory structure and the latter parts of the pathway, where flux branching occurs to produce alternative strigolactones, are less well-understood. Here we present a computational study that collects the available experimental evidence and proposes alternative biosynthetic pathways that are consistent with that evidence. Then, we test the alternative pathways through in silico simulation experiments and compare those experiments to experimental information. Our results predict the differences in dynamic behavior between alternative pathway designs. Independent of design, the analysis suggests that feedback regulation is unlikely to exist in strigolactone biosynthesis. In addition, our experiments suggest that engineering the pathway to modulate the production of strigolactones could be most easily achieved by increasing the flux of β-carotenes going into the biosynthetic pathway. Finally, we find that changing the ratio of alternative strigolactones produced by the pathway can be done by changing the activity of the enzymes after the flux branching points.
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spelling pubmed-94808292022-09-17 A mathematical model for strigolactone biosynthesis in plants Lucido, Abel Basallo, Oriol Sorribas, Albert Marin-Sanguino, Alberto Vilaprinyo, Ester Alves, Rui Front Plant Sci Plant Science Strigolactones mediate plant development, trigger symbiosis with arbuscular mycorrhizal fungi, are abundant in 80% of the plant kingdom and help plants gain resistance to environmental stressors. They also induce germination of parasitic plant seeds that are endemic to various continents, such as Orobanche in Europe or Asia and Striga in Africa. The genes involved in the early stages of strigolactones biosynthesis are known in several plants. The regulatory structure and the latter parts of the pathway, where flux branching occurs to produce alternative strigolactones, are less well-understood. Here we present a computational study that collects the available experimental evidence and proposes alternative biosynthetic pathways that are consistent with that evidence. Then, we test the alternative pathways through in silico simulation experiments and compare those experiments to experimental information. Our results predict the differences in dynamic behavior between alternative pathway designs. Independent of design, the analysis suggests that feedback regulation is unlikely to exist in strigolactone biosynthesis. In addition, our experiments suggest that engineering the pathway to modulate the production of strigolactones could be most easily achieved by increasing the flux of β-carotenes going into the biosynthetic pathway. Finally, we find that changing the ratio of alternative strigolactones produced by the pathway can be done by changing the activity of the enzymes after the flux branching points. Frontiers Media S.A. 2022-09-02 /pmc/articles/PMC9480829/ /pubmed/36119618 http://dx.doi.org/10.3389/fpls.2022.979162 Text en Copyright © 2022 Lucido, Basallo, Sorribas, Marin-Sanguino, Vilaprinyo and Alves. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lucido, Abel
Basallo, Oriol
Sorribas, Albert
Marin-Sanguino, Alberto
Vilaprinyo, Ester
Alves, Rui
A mathematical model for strigolactone biosynthesis in plants
title A mathematical model for strigolactone biosynthesis in plants
title_full A mathematical model for strigolactone biosynthesis in plants
title_fullStr A mathematical model for strigolactone biosynthesis in plants
title_full_unstemmed A mathematical model for strigolactone biosynthesis in plants
title_short A mathematical model for strigolactone biosynthesis in plants
title_sort mathematical model for strigolactone biosynthesis in plants
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480829/
https://www.ncbi.nlm.nih.gov/pubmed/36119618
http://dx.doi.org/10.3389/fpls.2022.979162
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