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Radical scavenging capacity, antibacterial activity, and quantum chemical aspects of the spectrophotometrically investigated iridium (III) complex with benzopyran derivative

A comprehensive aqueous phase spectrophotometric study concerning the trace level determination of iridium (III) by its reaction with benzopyran-derived chromogenic reagent, 6-chloro-3-hydroxy-7-methyl-2-(2′-thienyl)-4-oxo-4H-1-benzopyran (CHMTB), is performed. The complexing reagent instantly forms...

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Autores principales: Mohmad, Masrat, Agnihotri, Nivedita, Kumar, Vikas, Azam, Mohammad, Wabaidur, Saikh Mohammad, Kamal, Raj, Kumar, Rakesh, Alam, Mahboob, Kaviani, Sadegh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480850/
https://www.ncbi.nlm.nih.gov/pubmed/36120315
http://dx.doi.org/10.3389/fphar.2022.945323
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author Mohmad, Masrat
Agnihotri, Nivedita
Kumar, Vikas
Azam, Mohammad
Wabaidur, Saikh Mohammad
Kamal, Raj
Kumar, Rakesh
Alam, Mahboob
Kaviani, Sadegh
author_facet Mohmad, Masrat
Agnihotri, Nivedita
Kumar, Vikas
Azam, Mohammad
Wabaidur, Saikh Mohammad
Kamal, Raj
Kumar, Rakesh
Alam, Mahboob
Kaviani, Sadegh
author_sort Mohmad, Masrat
collection PubMed
description A comprehensive aqueous phase spectrophotometric study concerning the trace level determination of iridium (III) by its reaction with benzopyran-derived chromogenic reagent, 6-chloro-3-hydroxy-7-methyl-2-(2′-thienyl)-4-oxo-4H-1-benzopyran (CHMTB), is performed. The complexing reagent instantly forms a yellow complex with Ir (III) at pH 4.63, where metal is bound to the ligand in a ratio of 1:2 as deduced by Job’s continuous variations, mole ratio, and equilibrium shift methods. The complex absorbs maximally at 413–420 nm retaining its stability for up to 4 days. An optimum set of conditions have been set with respect to the parameters governing the formation of the complex. Under the set optimal conditions, the Ir (III)-CHMTB complex coheres to Beer’s law between 0.0 and 1.5 µg Ir (III) mL(−1). The attenuation coefficient and Sandell’s sensitivity are, respectively, 1.18×10(5) L mol(−1) cm(−1) and 0.00162 μg cm(−2) at 415 nm. The correlation coefficient (r) and standard deviation (SD) were 0.9999 and ± 0.001095, respectively, whereas the detection limit as analyzed was 0.007437 μg ml(−1). The interference with respect to analytically important cations and complexing agents has been studied thoroughly. It is found that the majority of the ions/agents do not intervene with the formation of the complex, thus adding to the versatility of the method. The results obtained from the aforesaid studies indicate a simple, fast, convenient, sensitive, and versatile method for microgram analysis of iridium (III) using CHMTB as a binding ligand. Furthermore, the studied complex is subjected to the evaluation of antibacterial and antioxidant capacity by employing the Agar Diffusion assay and DPPH(.) radical scavenging method, respectively. The results obtained from the mentioned assays reveal that the investigated complex possesses significant potency as an antibacterial and antioxidant agent. Finally, the computational approach through DFT of the formed complex confirmed the associated electronic properties of the studied complex.
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spelling pubmed-94808502022-09-17 Radical scavenging capacity, antibacterial activity, and quantum chemical aspects of the spectrophotometrically investigated iridium (III) complex with benzopyran derivative Mohmad, Masrat Agnihotri, Nivedita Kumar, Vikas Azam, Mohammad Wabaidur, Saikh Mohammad Kamal, Raj Kumar, Rakesh Alam, Mahboob Kaviani, Sadegh Front Pharmacol Pharmacology A comprehensive aqueous phase spectrophotometric study concerning the trace level determination of iridium (III) by its reaction with benzopyran-derived chromogenic reagent, 6-chloro-3-hydroxy-7-methyl-2-(2′-thienyl)-4-oxo-4H-1-benzopyran (CHMTB), is performed. The complexing reagent instantly forms a yellow complex with Ir (III) at pH 4.63, where metal is bound to the ligand in a ratio of 1:2 as deduced by Job’s continuous variations, mole ratio, and equilibrium shift methods. The complex absorbs maximally at 413–420 nm retaining its stability for up to 4 days. An optimum set of conditions have been set with respect to the parameters governing the formation of the complex. Under the set optimal conditions, the Ir (III)-CHMTB complex coheres to Beer’s law between 0.0 and 1.5 µg Ir (III) mL(−1). The attenuation coefficient and Sandell’s sensitivity are, respectively, 1.18×10(5) L mol(−1) cm(−1) and 0.00162 μg cm(−2) at 415 nm. The correlation coefficient (r) and standard deviation (SD) were 0.9999 and ± 0.001095, respectively, whereas the detection limit as analyzed was 0.007437 μg ml(−1). The interference with respect to analytically important cations and complexing agents has been studied thoroughly. It is found that the majority of the ions/agents do not intervene with the formation of the complex, thus adding to the versatility of the method. The results obtained from the aforesaid studies indicate a simple, fast, convenient, sensitive, and versatile method for microgram analysis of iridium (III) using CHMTB as a binding ligand. Furthermore, the studied complex is subjected to the evaluation of antibacterial and antioxidant capacity by employing the Agar Diffusion assay and DPPH(.) radical scavenging method, respectively. The results obtained from the mentioned assays reveal that the investigated complex possesses significant potency as an antibacterial and antioxidant agent. Finally, the computational approach through DFT of the formed complex confirmed the associated electronic properties of the studied complex. Frontiers Media S.A. 2022-09-02 /pmc/articles/PMC9480850/ /pubmed/36120315 http://dx.doi.org/10.3389/fphar.2022.945323 Text en Copyright © 2022 Mohmad, Agnihotri, Kumar, Azam, Wabaidur, Kamal, Kumar, Alam and Kaviani. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Mohmad, Masrat
Agnihotri, Nivedita
Kumar, Vikas
Azam, Mohammad
Wabaidur, Saikh Mohammad
Kamal, Raj
Kumar, Rakesh
Alam, Mahboob
Kaviani, Sadegh
Radical scavenging capacity, antibacterial activity, and quantum chemical aspects of the spectrophotometrically investigated iridium (III) complex with benzopyran derivative
title Radical scavenging capacity, antibacterial activity, and quantum chemical aspects of the spectrophotometrically investigated iridium (III) complex with benzopyran derivative
title_full Radical scavenging capacity, antibacterial activity, and quantum chemical aspects of the spectrophotometrically investigated iridium (III) complex with benzopyran derivative
title_fullStr Radical scavenging capacity, antibacterial activity, and quantum chemical aspects of the spectrophotometrically investigated iridium (III) complex with benzopyran derivative
title_full_unstemmed Radical scavenging capacity, antibacterial activity, and quantum chemical aspects of the spectrophotometrically investigated iridium (III) complex with benzopyran derivative
title_short Radical scavenging capacity, antibacterial activity, and quantum chemical aspects of the spectrophotometrically investigated iridium (III) complex with benzopyran derivative
title_sort radical scavenging capacity, antibacterial activity, and quantum chemical aspects of the spectrophotometrically investigated iridium (iii) complex with benzopyran derivative
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480850/
https://www.ncbi.nlm.nih.gov/pubmed/36120315
http://dx.doi.org/10.3389/fphar.2022.945323
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