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RAPID IMPLEMENTATION OF REAL-TIME REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION (REAL-TIME RT-PCR) ASSAY FOR THE DETECTION OF SARS-COV-2 IN A MOROCCAN HOSPITAL

BACKGROUND: The main challenge faced in the African countries was to implement efficient molecular diagnostic facilities and start Covid-19 diagnosis as fast as possible to handle the rapid and unpredictable rise of cases. MATERIALS, METHODS AND RESULTS: We describe our experience in implementing a...

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Detalles Bibliográficos
Autores principales: Bouchra, Belefquih, Wissal, Maher, Amine, Cheikh, Wail, Hamdani, Younes, Zaid, Hakima, Kabbaj, Meryem, Seffar, Hanae, Dakka, Khaoula, Hadami, Afaf, Allaoui, Amina, Benouda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480888/
https://www.ncbi.nlm.nih.gov/pubmed/36124329
http://dx.doi.org/10.21010/Ajid.v16i2S.8
Descripción
Sumario:BACKGROUND: The main challenge faced in the African countries was to implement efficient molecular diagnostic facilities and start Covid-19 diagnosis as fast as possible to handle the rapid and unpredictable rise of cases. MATERIALS, METHODS AND RESULTS: We describe our experience in implementing a molecular biology unit at Sheikh Zaïd International University Hospital in Rabat, with a delay as short as one week, and starting real-time RT-PCR assay for the detection of SARS-Cov-2 infection, since the outbreak widened in Morocco in mid-March, 2020. CONCLUSION: The challenges encountered in the first period of Covid-19 pandemic are still present. This work aims to give an example of a rapid and adaptive response in order to maintain our diagnosis ability for Covid-19 and for other pathogens.