RAPID IMPLEMENTATION OF REAL-TIME REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION (REAL-TIME RT-PCR) ASSAY FOR THE DETECTION OF SARS-COV-2 IN A MOROCCAN HOSPITAL

BACKGROUND: The main challenge faced in the African countries was to implement efficient molecular diagnostic facilities and start Covid-19 diagnosis as fast as possible to handle the rapid and unpredictable rise of cases. MATERIALS, METHODS AND RESULTS: We describe our experience in implementing a...

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Autores principales: Bouchra, Belefquih, Wissal, Maher, Amine, Cheikh, Wail, Hamdani, Younes, Zaid, Hakima, Kabbaj, Meryem, Seffar, Hanae, Dakka, Khaoula, Hadami, Afaf, Allaoui, Amina, Benouda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480888/
https://www.ncbi.nlm.nih.gov/pubmed/36124329
http://dx.doi.org/10.21010/Ajid.v16i2S.8
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author Bouchra, Belefquih
Wissal, Maher
Amine, Cheikh
Wail, Hamdani
Younes, Zaid
Hakima, Kabbaj
Meryem, Seffar
Hanae, Dakka
Khaoula, Hadami
Afaf, Allaoui
Amina, Benouda
author_facet Bouchra, Belefquih
Wissal, Maher
Amine, Cheikh
Wail, Hamdani
Younes, Zaid
Hakima, Kabbaj
Meryem, Seffar
Hanae, Dakka
Khaoula, Hadami
Afaf, Allaoui
Amina, Benouda
author_sort Bouchra, Belefquih
collection PubMed
description BACKGROUND: The main challenge faced in the African countries was to implement efficient molecular diagnostic facilities and start Covid-19 diagnosis as fast as possible to handle the rapid and unpredictable rise of cases. MATERIALS, METHODS AND RESULTS: We describe our experience in implementing a molecular biology unit at Sheikh Zaïd International University Hospital in Rabat, with a delay as short as one week, and starting real-time RT-PCR assay for the detection of SARS-Cov-2 infection, since the outbreak widened in Morocco in mid-March, 2020. CONCLUSION: The challenges encountered in the first period of Covid-19 pandemic are still present. This work aims to give an example of a rapid and adaptive response in order to maintain our diagnosis ability for Covid-19 and for other pathogens.
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spelling pubmed-94808882022-09-18 RAPID IMPLEMENTATION OF REAL-TIME REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION (REAL-TIME RT-PCR) ASSAY FOR THE DETECTION OF SARS-COV-2 IN A MOROCCAN HOSPITAL Bouchra, Belefquih Wissal, Maher Amine, Cheikh Wail, Hamdani Younes, Zaid Hakima, Kabbaj Meryem, Seffar Hanae, Dakka Khaoula, Hadami Afaf, Allaoui Amina, Benouda Afr J Infect Dis Article BACKGROUND: The main challenge faced in the African countries was to implement efficient molecular diagnostic facilities and start Covid-19 diagnosis as fast as possible to handle the rapid and unpredictable rise of cases. MATERIALS, METHODS AND RESULTS: We describe our experience in implementing a molecular biology unit at Sheikh Zaïd International University Hospital in Rabat, with a delay as short as one week, and starting real-time RT-PCR assay for the detection of SARS-Cov-2 infection, since the outbreak widened in Morocco in mid-March, 2020. CONCLUSION: The challenges encountered in the first period of Covid-19 pandemic are still present. This work aims to give an example of a rapid and adaptive response in order to maintain our diagnosis ability for Covid-19 and for other pathogens. African Traditional Herbal Medicine Supporters Initiative (ATHMSI) 2022-08-17 /pmc/articles/PMC9480888/ /pubmed/36124329 http://dx.doi.org/10.21010/Ajid.v16i2S.8 Text en Copyright: © 2022 Afr. J. Infect. Diseases https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
spellingShingle Article
Bouchra, Belefquih
Wissal, Maher
Amine, Cheikh
Wail, Hamdani
Younes, Zaid
Hakima, Kabbaj
Meryem, Seffar
Hanae, Dakka
Khaoula, Hadami
Afaf, Allaoui
Amina, Benouda
RAPID IMPLEMENTATION OF REAL-TIME REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION (REAL-TIME RT-PCR) ASSAY FOR THE DETECTION OF SARS-COV-2 IN A MOROCCAN HOSPITAL
title RAPID IMPLEMENTATION OF REAL-TIME REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION (REAL-TIME RT-PCR) ASSAY FOR THE DETECTION OF SARS-COV-2 IN A MOROCCAN HOSPITAL
title_full RAPID IMPLEMENTATION OF REAL-TIME REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION (REAL-TIME RT-PCR) ASSAY FOR THE DETECTION OF SARS-COV-2 IN A MOROCCAN HOSPITAL
title_fullStr RAPID IMPLEMENTATION OF REAL-TIME REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION (REAL-TIME RT-PCR) ASSAY FOR THE DETECTION OF SARS-COV-2 IN A MOROCCAN HOSPITAL
title_full_unstemmed RAPID IMPLEMENTATION OF REAL-TIME REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION (REAL-TIME RT-PCR) ASSAY FOR THE DETECTION OF SARS-COV-2 IN A MOROCCAN HOSPITAL
title_short RAPID IMPLEMENTATION OF REAL-TIME REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION (REAL-TIME RT-PCR) ASSAY FOR THE DETECTION OF SARS-COV-2 IN A MOROCCAN HOSPITAL
title_sort rapid implementation of real-time reverse-transcription polymerase chain reaction (real-time rt-pcr) assay for the detection of sars-cov-2 in a moroccan hospital
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480888/
https://www.ncbi.nlm.nih.gov/pubmed/36124329
http://dx.doi.org/10.21010/Ajid.v16i2S.8
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